<?xml version="1.0" encoding="UTF-8"?>
<STUDY_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <STUDY center_name="BioProject" alias="PRJNA743555" accession="SRP326817">
    <IDENTIFIERS>
      <PRIMARY_ID>SRP326817</PRIMARY_ID>
      <EXTERNAL_ID namespace="BioProject" label="primary">PRJNA743555</EXTERNAL_ID>
    </IDENTIFIERS>
    <DESCRIPTOR>
      <STUDY_TITLE>Capped-small RNA-sequencing (csRNA-seq) of populations of poeciliid fish from hydrogen-sulfide rich and non-sulfidic springs in Mexico</STUDY_TITLE>
      <STUDY_TYPE existing_study_type="Other"/>
      <STUDY_ABSTRACT>We performed capped-small RNA-sequencing (csRNA-seq) on 12 individuals of Poecilia spp. sampled from both sulfidic and non-sulfidic springs in southern Mexico (3 non-sulfidic populations, 3 sulfidic populations, 2 individuals from each site). csRNA-seq captures short (~20-60 nucleotides) stable and unstable RNA transcripts. Each fish sample has a "csRNA" file that contains the sequences of only 5' capped small RNAs and an "input" file that contains total RNA sequences as a way to eliminate false positives. See Duttke et al. 2019 "Identification and dynamic quantification of regulatory elements using total RNA" in Genome Research for a full description of csRNA-seq.</STUDY_ABSTRACT>
    </DESCRIPTOR>
  </STUDY>
</STUDY_SET>
