SRX11399573 GSM5434818 SRP327666 SRS9442150 GSM5434818 RIP-Seq TRANSCRIPTOMIC other Total RNA was isolated and purified using TRIzol reagent (cat#15596018, Invitrogen, Carlsbad, CA, USA). RNA integrity was assessed using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Samples were used only if RNA integrity number (RIN) was >7.0 and appeared high-quality on denaturing agarose gel electrophoresis. Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT)25 (cat# 61005, Thermo Fisher Scientific), and fragmented into small pieces at 86 ℃ for 7 min using Magnesium RNA Fragmentation Module (cat# e6150, New England Biolabs, Ipswich, MA, USA). The cleaved RNA fragments were incubated at 4 ℃ for 2 h with an m6A-specific antibody (cat# 202003, Synaptic Systems, Göttingen, Niedersachsen, Germany) in 50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630. Immunoprecipitated RNA was reverse-transcribed to cDNA by SuperScript™ II Reverse Transcriptase (cat# 1896649, Invitrogen). The cDNA was then used to synthesize U-labeled second-stranded DNAs with E. coli DNA polymerase I (cat# m0209, New England Biolabs), RNase H (cat# m0297, New England Biolabs) and dUTP (cat# R0133, Thermo Fisher Scientific). A-base was then added to the blunt ends of each strand, preparing them for ligation to the indexed adapters. Each adapter contained a T-base overhang for ligating the adapter to the A-tailed fragmented DNA. Single- or dual-index adapters were ligated to the fragments, and size selection was performed with AMPureXP beads. After treatment with a heat-labile UDG enzyme (cat# m0280, New England Biolabs), the ligated products were amplified by PCR under the following conditions: initial denaturation at 95 ℃ for 3 min; 8 cycles of denaturation at 98 ℃ for 15 sec, annealing at 60 ℃ for 15 sec, and extension at 72 ℃ for 30 sec; and then final extension at 72 ℃ for 5 min. The average insert size for the final cDNA library was 300±50 bp. We then performed the 2×150-bp paired-end sequencing (PE150) on an illumine Novaseq™ 6000 (LC-Bio Technology, Hangzhou, Zhejiang, China). Illumina NovaSeq 6000 gds 305434818 GSM5434818 GEO Accession GSM5434818 SRX11399574 GSM5434819 SRP327666 SRS9442153 GSM5434819 RIP-Seq TRANSCRIPTOMIC other Total RNA was isolated and purified using TRIzol reagent (cat#15596018, Invitrogen, Carlsbad, CA, USA). RNA integrity was assessed using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Samples were used only if RNA integrity number (RIN) was >7.0 and appeared high-quality on denaturing agarose gel electrophoresis. Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT)25 (cat# 61005, Thermo Fisher Scientific), and fragmented into small pieces at 86 ℃ for 7 min using Magnesium RNA Fragmentation Module (cat# e6150, New England Biolabs, Ipswich, MA, USA). The cleaved RNA fragments were incubated at 4 ℃ for 2 h with an m6A-specific antibody (cat# 202003, Synaptic Systems, Göttingen, Niedersachsen, Germany) in 50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630. Immunoprecipitated RNA was reverse-transcribed to cDNA by SuperScript™ II Reverse Transcriptase (cat# 1896649, Invitrogen). The cDNA was then used to synthesize U-labeled second-stranded DNAs with E. coli DNA polymerase I (cat# m0209, New England Biolabs), RNase H (cat# m0297, New England Biolabs) and dUTP (cat# R0133, Thermo Fisher Scientific). A-base was then added to the blunt ends of each strand, preparing them for ligation to the indexed adapters. Each adapter contained a T-base overhang for ligating the adapter to the A-tailed fragmented DNA. Single- or dual-index adapters were ligated to the fragments, and size selection was performed with AMPureXP beads. After treatment with a heat-labile UDG enzyme (cat# m0280, New England Biolabs), the ligated products were amplified by PCR under the following conditions: initial denaturation at 95 ℃ for 3 min; 8 cycles of denaturation at 98 ℃ for 15 sec, annealing at 60 ℃ for 15 sec, and extension at 72 ℃ for 30 sec; and then final extension at 72 ℃ for 5 min. The average insert size for the final cDNA library was 300±50 bp. We then performed the 2×150-bp paired-end sequencing (PE150) on an illumine Novaseq™ 6000 (LC-Bio Technology, Hangzhou, Zhejiang, China). Illumina NovaSeq 6000 gds 305434819 GSM5434819 GEO Accession GSM5434819 SRX11399575 GSM5434820 SRP327666 SRS9442151 GSM5434820 RIP-Seq TRANSCRIPTOMIC other Total RNA was isolated and purified using TRIzol reagent (cat#15596018, Invitrogen, Carlsbad, CA, USA). RNA integrity was assessed using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Samples were used only if RNA integrity number (RIN) was >7.0 and appeared high-quality on denaturing agarose gel electrophoresis. Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT)25 (cat# 61005, Thermo Fisher Scientific), and fragmented into small pieces at 86 ℃ for 7 min using Magnesium RNA Fragmentation Module (cat# e6150, New England Biolabs, Ipswich, MA, USA). The cleaved RNA fragments were incubated at 4 ℃ for 2 h with an m6A-specific antibody (cat# 202003, Synaptic Systems, Göttingen, Niedersachsen, Germany) in 50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630. Immunoprecipitated RNA was reverse-transcribed to cDNA by SuperScript™ II Reverse Transcriptase (cat# 1896649, Invitrogen). The cDNA was then used to synthesize U-labeled second-stranded DNAs with E. coli DNA polymerase I (cat# m0209, New England Biolabs), RNase H (cat# m0297, New England Biolabs) and dUTP (cat# R0133, Thermo Fisher Scientific). A-base was then added to the blunt ends of each strand, preparing them for ligation to the indexed adapters. Each adapter contained a T-base overhang for ligating the adapter to the A-tailed fragmented DNA. Single- or dual-index adapters were ligated to the fragments, and size selection was performed with AMPureXP beads. After treatment with a heat-labile UDG enzyme (cat# m0280, New England Biolabs), the ligated products were amplified by PCR under the following conditions: initial denaturation at 95 ℃ for 3 min; 8 cycles of denaturation at 98 ℃ for 15 sec, annealing at 60 ℃ for 15 sec, and extension at 72 ℃ for 30 sec; and then final extension at 72 ℃ for 5 min. The average insert size for the final cDNA library was 300±50 bp. We then performed the 2×150-bp paired-end sequencing (PE150) on an illumine Novaseq™ 6000 (LC-Bio Technology, Hangzhou, Zhejiang, China). Illumina NovaSeq 6000 gds 305434820 GSM5434820 GEO Accession GSM5434820 SRX11399576 GSM5434821 SRP327666 SRS9442152 GSM5434821 RIP-Seq TRANSCRIPTOMIC other Total RNA was isolated and purified using TRIzol reagent (cat#15596018, Invitrogen, Carlsbad, CA, USA). RNA integrity was assessed using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Samples were used only if RNA integrity number (RIN) was >7.0 and appeared high-quality on denaturing agarose gel electrophoresis. Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT)25 (cat# 61005, Thermo Fisher Scientific), and fragmented into small pieces at 86 ℃ for 7 min using Magnesium RNA Fragmentation Module (cat# e6150, New England Biolabs, Ipswich, MA, USA). The cleaved RNA fragments were incubated at 4 ℃ for 2 h with an m6A-specific antibody (cat# 202003, Synaptic Systems, Göttingen, Niedersachsen, Germany) in 50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630. Immunoprecipitated RNA was reverse-transcribed to cDNA by SuperScript™ II Reverse Transcriptase (cat# 1896649, Invitrogen). The cDNA was then used to synthesize U-labeled second-stranded DNAs with E. coli DNA polymerase I (cat# m0209, New England Biolabs), RNase H (cat# m0297, New England Biolabs) and dUTP (cat# R0133, Thermo Fisher Scientific). A-base was then added to the blunt ends of each strand, preparing them for ligation to the indexed adapters. Each adapter contained a T-base overhang for ligating the adapter to the A-tailed fragmented DNA. Single- or dual-index adapters were ligated to the fragments, and size selection was performed with AMPureXP beads. After treatment with a heat-labile UDG enzyme (cat# m0280, New England Biolabs), the ligated products were amplified by PCR under the following conditions: initial denaturation at 95 ℃ for 3 min; 8 cycles of denaturation at 98 ℃ for 15 sec, annealing at 60 ℃ for 15 sec, and extension at 72 ℃ for 30 sec; and then final extension at 72 ℃ for 5 min. The average insert size for the final cDNA library was 300±50 bp. We then performed the 2×150-bp paired-end sequencing (PE150) on an illumine Novaseq™ 6000 (LC-Bio Technology, Hangzhou, Zhejiang, China). Illumina NovaSeq 6000 gds 305434821 GSM5434821 GEO Accession GSM5434821 SRX11399577 GSM5434822 SRP327666 SRS9442155 GSM5434822 RIP-Seq TRANSCRIPTOMIC other Total RNA was isolated and purified using TRIzol reagent (cat#15596018, Invitrogen, Carlsbad, CA, USA). RNA integrity was assessed using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Samples were used only if RNA integrity number (RIN) was >7.0 and appeared high-quality on denaturing agarose gel electrophoresis. Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT)25 (cat# 61005, Thermo Fisher Scientific), and fragmented into small pieces at 86 ℃ for 7 min using Magnesium RNA Fragmentation Module (cat# e6150, New England Biolabs, Ipswich, MA, USA). The cleaved RNA fragments were incubated at 4 ℃ for 2 h with an m6A-specific antibody (cat# 202003, Synaptic Systems, Göttingen, Niedersachsen, Germany) in 50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630. Immunoprecipitated RNA was reverse-transcribed to cDNA by SuperScript™ II Reverse Transcriptase (cat# 1896649, Invitrogen). The cDNA was then used to synthesize U-labeled second-stranded DNAs with E. coli DNA polymerase I (cat# m0209, New England Biolabs), RNase H (cat# m0297, New England Biolabs) and dUTP (cat# R0133, Thermo Fisher Scientific). A-base was then added to the blunt ends of each strand, preparing them for ligation to the indexed adapters. Each adapter contained a T-base overhang for ligating the adapter to the A-tailed fragmented DNA. Single- or dual-index adapters were ligated to the fragments, and size selection was performed with AMPureXP beads. After treatment with a heat-labile UDG enzyme (cat# m0280, New England Biolabs), the ligated products were amplified by PCR under the following conditions: initial denaturation at 95 ℃ for 3 min; 8 cycles of denaturation at 98 ℃ for 15 sec, annealing at 60 ℃ for 15 sec, and extension at 72 ℃ for 30 sec; and then final extension at 72 ℃ for 5 min. The average insert size for the final cDNA library was 300±50 bp. We then performed the 2×150-bp paired-end sequencing (PE150) on an illumine Novaseq™ 6000 (LC-Bio Technology, Hangzhou, Zhejiang, China). Illumina NovaSeq 6000 gds 305434822 GSM5434822 GEO Accession GSM5434822 SRX11399578 GSM5434823 SRP327666 SRS9442154 GSM5434823 RIP-Seq TRANSCRIPTOMIC other Total RNA was isolated and purified using TRIzol reagent (cat#15596018, Invitrogen, Carlsbad, CA, USA). RNA integrity was assessed using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Samples were used only if RNA integrity number (RIN) was >7.0 and appeared high-quality on denaturing agarose gel electrophoresis. Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT)25 (cat# 61005, Thermo Fisher Scientific), and fragmented into small pieces at 86 ℃ for 7 min using Magnesium RNA Fragmentation Module (cat# e6150, New England Biolabs, Ipswich, MA, USA). The cleaved RNA fragments were incubated at 4 ℃ for 2 h with an m6A-specific antibody (cat# 202003, Synaptic Systems, Göttingen, Niedersachsen, Germany) in 50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630. Immunoprecipitated RNA was reverse-transcribed to cDNA by SuperScript™ II Reverse Transcriptase (cat# 1896649, Invitrogen). The cDNA was then used to synthesize U-labeled second-stranded DNAs with E. coli DNA polymerase I (cat# m0209, New England Biolabs), RNase H (cat# m0297, New England Biolabs) and dUTP (cat# R0133, Thermo Fisher Scientific). A-base was then added to the blunt ends of each strand, preparing them for ligation to the indexed adapters. Each adapter contained a T-base overhang for ligating the adapter to the A-tailed fragmented DNA. Single- or dual-index adapters were ligated to the fragments, and size selection was performed with AMPureXP beads. After treatment with a heat-labile UDG enzyme (cat# m0280, New England Biolabs), the ligated products were amplified by PCR under the following conditions: initial denaturation at 95 ℃ for 3 min; 8 cycles of denaturation at 98 ℃ for 15 sec, annealing at 60 ℃ for 15 sec, and extension at 72 ℃ for 30 sec; and then final extension at 72 ℃ for 5 min. The average insert size for the final cDNA library was 300±50 bp. We then performed the 2×150-bp paired-end sequencing (PE150) on an illumine Novaseq™ 6000 (LC-Bio Technology, Hangzhou, Zhejiang, China). Illumina NovaSeq 6000 gds 305434823 GSM5434823 GEO Accession GSM5434823 SRX11399579 GSM5434824 SRP327666 SRS9442157 GSM5434824 RIP-Seq TRANSCRIPTOMIC other Total RNA was isolated and purified using TRIzol reagent (cat#15596018, Invitrogen, Carlsbad, CA, USA). RNA integrity was assessed using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Samples were used only if RNA integrity number (RIN) was >7.0 and appeared high-quality on denaturing agarose gel electrophoresis. Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT)25 (cat# 61005, Thermo Fisher Scientific), and fragmented into small pieces at 86 ℃ for 7 min using Magnesium RNA Fragmentation Module (cat# e6150, New England Biolabs, Ipswich, MA, USA). The cleaved RNA fragments were incubated at 4 ℃ for 2 h with an m6A-specific antibody (cat# 202003, Synaptic Systems, Göttingen, Niedersachsen, Germany) in 50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630. Immunoprecipitated RNA was reverse-transcribed to cDNA by SuperScript™ II Reverse Transcriptase (cat# 1896649, Invitrogen). The cDNA was then used to synthesize U-labeled second-stranded DNAs with E. coli DNA polymerase I (cat# m0209, New England Biolabs), RNase H (cat# m0297, New England Biolabs) and dUTP (cat# R0133, Thermo Fisher Scientific). A-base was then added to the blunt ends of each strand, preparing them for ligation to the indexed adapters. Each adapter contained a T-base overhang for ligating the adapter to the A-tailed fragmented DNA. Single- or dual-index adapters were ligated to the fragments, and size selection was performed with AMPureXP beads. After treatment with a heat-labile UDG enzyme (cat# m0280, New England Biolabs), the ligated products were amplified by PCR under the following conditions: initial denaturation at 95 ℃ for 3 min; 8 cycles of denaturation at 98 ℃ for 15 sec, annealing at 60 ℃ for 15 sec, and extension at 72 ℃ for 30 sec; and then final extension at 72 ℃ for 5 min. The average insert size for the final cDNA library was 300±50 bp. We then performed the 2×150-bp paired-end sequencing (PE150) on an illumine Novaseq™ 6000 (LC-Bio Technology, Hangzhou, Zhejiang, China). Illumina NovaSeq 6000 gds 305434824 GSM5434824 GEO Accession GSM5434824 SRX11399580 GSM5434825 SRP327666 SRS9442156 GSM5434825 RIP-Seq TRANSCRIPTOMIC other Total RNA was isolated and purified using TRIzol reagent (cat#15596018, Invitrogen, Carlsbad, CA, USA). RNA integrity was assessed using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Samples were used only if RNA integrity number (RIN) was >7.0 and appeared high-quality on denaturing agarose gel electrophoresis. Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT)25 (cat# 61005, Thermo Fisher Scientific), and fragmented into small pieces at 86 ℃ for 7 min using Magnesium RNA Fragmentation Module (cat# e6150, New England Biolabs, Ipswich, MA, USA). The cleaved RNA fragments were incubated at 4 ℃ for 2 h with an m6A-specific antibody (cat# 202003, Synaptic Systems, Göttingen, Niedersachsen, Germany) in 50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630. Immunoprecipitated RNA was reverse-transcribed to cDNA by SuperScript™ II Reverse Transcriptase (cat# 1896649, Invitrogen). The cDNA was then used to synthesize U-labeled second-stranded DNAs with E. coli DNA polymerase I (cat# m0209, New England Biolabs), RNase H (cat# m0297, New England Biolabs) and dUTP (cat# R0133, Thermo Fisher Scientific). A-base was then added to the blunt ends of each strand, preparing them for ligation to the indexed adapters. Each adapter contained a T-base overhang for ligating the adapter to the A-tailed fragmented DNA. Single- or dual-index adapters were ligated to the fragments, and size selection was performed with AMPureXP beads. After treatment with a heat-labile UDG enzyme (cat# m0280, New England Biolabs), the ligated products were amplified by PCR under the following conditions: initial denaturation at 95 ℃ for 3 min; 8 cycles of denaturation at 98 ℃ for 15 sec, annealing at 60 ℃ for 15 sec, and extension at 72 ℃ for 30 sec; and then final extension at 72 ℃ for 5 min. The average insert size for the final cDNA library was 300±50 bp. We then performed the 2×150-bp paired-end sequencing (PE150) on an illumine Novaseq™ 6000 (LC-Bio Technology, Hangzhou, Zhejiang, China). Illumina NovaSeq 6000 gds 305434825 GSM5434825 GEO Accession GSM5434825 SRX11399581 GSM5434826 SRP327666 SRS9442158 GSM5434826 RIP-Seq TRANSCRIPTOMIC other Total RNA was isolated and purified using TRIzol reagent (cat#15596018, Invitrogen, Carlsbad, CA, USA). RNA integrity was assessed using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Samples were used only if RNA integrity number (RIN) was >7.0 and appeared high-quality on denaturing agarose gel electrophoresis. Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT)25 (cat# 61005, Thermo Fisher Scientific), and fragmented into small pieces at 86 ℃ for 7 min using Magnesium RNA Fragmentation Module (cat# e6150, New England Biolabs, Ipswich, MA, USA). The cleaved RNA fragments were incubated at 4 ℃ for 2 h with an m6A-specific antibody (cat# 202003, Synaptic Systems, Göttingen, Niedersachsen, Germany) in 50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630. Immunoprecipitated RNA was reverse-transcribed to cDNA by SuperScript™ II Reverse Transcriptase (cat# 1896649, Invitrogen). The cDNA was then used to synthesize U-labeled second-stranded DNAs with E. coli DNA polymerase I (cat# m0209, New England Biolabs), RNase H (cat# m0297, New England Biolabs) and dUTP (cat# R0133, Thermo Fisher Scientific). A-base was then added to the blunt ends of each strand, preparing them for ligation to the indexed adapters. Each adapter contained a T-base overhang for ligating the adapter to the A-tailed fragmented DNA. Single- or dual-index adapters were ligated to the fragments, and size selection was performed with AMPureXP beads. After treatment with a heat-labile UDG enzyme (cat# m0280, New England Biolabs), the ligated products were amplified by PCR under the following conditions: initial denaturation at 95 ℃ for 3 min; 8 cycles of denaturation at 98 ℃ for 15 sec, annealing at 60 ℃ for 15 sec, and extension at 72 ℃ for 30 sec; and then final extension at 72 ℃ for 5 min. The average insert size for the final cDNA library was 300±50 bp. We then performed the 2×150-bp paired-end sequencing (PE150) on an illumine Novaseq™ 6000 (LC-Bio Technology, Hangzhou, Zhejiang, China). Illumina NovaSeq 6000 gds 305434826 GSM5434826 GEO Accession GSM5434826 SRX11399582 GSM5434827 SRP327666 SRS9442159 GSM5434827 RIP-Seq TRANSCRIPTOMIC other Total RNA was isolated and purified using TRIzol reagent (cat#15596018, Invitrogen, Carlsbad, CA, USA). RNA integrity was assessed using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Samples were used only if RNA integrity number (RIN) was >7.0 and appeared high-quality on denaturing agarose gel electrophoresis. Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT)25 (cat# 61005, Thermo Fisher Scientific), and fragmented into small pieces at 86 ℃ for 7 min using Magnesium RNA Fragmentation Module (cat# e6150, New England Biolabs, Ipswich, MA, USA). The cleaved RNA fragments were incubated at 4 ℃ for 2 h with an m6A-specific antibody (cat# 202003, Synaptic Systems, Göttingen, Niedersachsen, Germany) in 50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630. Immunoprecipitated RNA was reverse-transcribed to cDNA by SuperScript™ II Reverse Transcriptase (cat# 1896649, Invitrogen). The cDNA was then used to synthesize U-labeled second-stranded DNAs with E. coli DNA polymerase I (cat# m0209, New England Biolabs), RNase H (cat# m0297, New England Biolabs) and dUTP (cat# R0133, Thermo Fisher Scientific). A-base was then added to the blunt ends of each strand, preparing them for ligation to the indexed adapters. Each adapter contained a T-base overhang for ligating the adapter to the A-tailed fragmented DNA. Single- or dual-index adapters were ligated to the fragments, and size selection was performed with AMPureXP beads. After treatment with a heat-labile UDG enzyme (cat# m0280, New England Biolabs), the ligated products were amplified by PCR under the following conditions: initial denaturation at 95 ℃ for 3 min; 8 cycles of denaturation at 98 ℃ for 15 sec, annealing at 60 ℃ for 15 sec, and extension at 72 ℃ for 30 sec; and then final extension at 72 ℃ for 5 min. The average insert size for the final cDNA library was 300±50 bp. We then performed the 2×150-bp paired-end sequencing (PE150) on an illumine Novaseq™ 6000 (LC-Bio Technology, Hangzhou, Zhejiang, China). Illumina NovaSeq 6000 gds 305434827 GSM5434827 GEO Accession GSM5434827 SRX11399583 GSM5434828 SRP327666 SRS9442160 GSM5434828 RIP-Seq TRANSCRIPTOMIC other Total RNA was isolated and purified using TRIzol reagent (cat#15596018, Invitrogen, Carlsbad, CA, USA). RNA integrity was assessed using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Samples were used only if RNA integrity number (RIN) was >7.0 and appeared high-quality on denaturing agarose gel electrophoresis. Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT)25 (cat# 61005, Thermo Fisher Scientific), and fragmented into small pieces at 86 ℃ for 7 min using Magnesium RNA Fragmentation Module (cat# e6150, New England Biolabs, Ipswich, MA, USA). The cleaved RNA fragments were incubated at 4 ℃ for 2 h with an m6A-specific antibody (cat# 202003, Synaptic Systems, Göttingen, Niedersachsen, Germany) in 50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630. Immunoprecipitated RNA was reverse-transcribed to cDNA by SuperScript™ II Reverse Transcriptase (cat# 1896649, Invitrogen). The cDNA was then used to synthesize U-labeled second-stranded DNAs with E. coli DNA polymerase I (cat# m0209, New England Biolabs), RNase H (cat# m0297, New England Biolabs) and dUTP (cat# R0133, Thermo Fisher Scientific). A-base was then added to the blunt ends of each strand, preparing them for ligation to the indexed adapters. Each adapter contained a T-base overhang for ligating the adapter to the A-tailed fragmented DNA. Single- or dual-index adapters were ligated to the fragments, and size selection was performed with AMPureXP beads. After treatment with a heat-labile UDG enzyme (cat# m0280, New England Biolabs), the ligated products were amplified by PCR under the following conditions: initial denaturation at 95 ℃ for 3 min; 8 cycles of denaturation at 98 ℃ for 15 sec, annealing at 60 ℃ for 15 sec, and extension at 72 ℃ for 30 sec; and then final extension at 72 ℃ for 5 min. The average insert size for the final cDNA library was 300±50 bp. We then performed the 2×150-bp paired-end sequencing (PE150) on an illumine Novaseq™ 6000 (LC-Bio Technology, Hangzhou, Zhejiang, China). Illumina NovaSeq 6000 gds 305434828 GSM5434828 GEO Accession GSM5434828 SRX11399584 GSM5434829 SRP327666 SRS9442161 GSM5434829 RIP-Seq TRANSCRIPTOMIC other Total RNA was isolated and purified using TRIzol reagent (cat#15596018, Invitrogen, Carlsbad, CA, USA). RNA integrity was assessed using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Samples were used only if RNA integrity number (RIN) was >7.0 and appeared high-quality on denaturing agarose gel electrophoresis. Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT)25 (cat# 61005, Thermo Fisher Scientific), and fragmented into small pieces at 86 ℃ for 7 min using Magnesium RNA Fragmentation Module (cat# e6150, New England Biolabs, Ipswich, MA, USA). The cleaved RNA fragments were incubated at 4 ℃ for 2 h with an m6A-specific antibody (cat# 202003, Synaptic Systems, Göttingen, Niedersachsen, Germany) in 50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630. Immunoprecipitated RNA was reverse-transcribed to cDNA by SuperScript™ II Reverse Transcriptase (cat# 1896649, Invitrogen). The cDNA was then used to synthesize U-labeled second-stranded DNAs with E. coli DNA polymerase I (cat# m0209, New England Biolabs), RNase H (cat# m0297, New England Biolabs) and dUTP (cat# R0133, Thermo Fisher Scientific). A-base was then added to the blunt ends of each strand, preparing them for ligation to the indexed adapters. Each adapter contained a T-base overhang for ligating the adapter to the A-tailed fragmented DNA. Single- or dual-index adapters were ligated to the fragments, and size selection was performed with AMPureXP beads. After treatment with a heat-labile UDG enzyme (cat# m0280, New England Biolabs), the ligated products were amplified by PCR under the following conditions: initial denaturation at 95 ℃ for 3 min; 8 cycles of denaturation at 98 ℃ for 15 sec, annealing at 60 ℃ for 15 sec, and extension at 72 ℃ for 30 sec; and then final extension at 72 ℃ for 5 min. The average insert size for the final cDNA library was 300±50 bp. We then performed the 2×150-bp paired-end sequencing (PE150) on an illumine Novaseq™ 6000 (LC-Bio Technology, Hangzhou, Zhejiang, China). Illumina NovaSeq 6000 gds 305434829 GSM5434829 GEO Accession GSM5434829