SRX11410629 GSM5436907 SRP327939 SRS9454264 GSM5436907 RNA-Seq TRANSCRIPTOMIC cDNA Cells were grown overnight, then normalized to OD 0.06 and let grow to OD 0.5 and 0.8. Cells were pelleted and snap frozen for RNA extraction with the Directzol RNA mini-prep plus kit (Zymo Research, R2071). The samples were also DNase treated with TURBO DNase (Thermo Fisher Scientific, AM2239), rRNA was removed with Rybo-off rRNA depletion kit (Vazyme Biotech, N407-01) and then sequenced with NovaSeq 6000 (Illumina: flow cell S1, 100 cycles) using a 50 bp paired-end read. NEBNext Ultra II Directional RNA Illumina NovaSeq 6000 gds 305436907 GSM5436907 GEO Accession GSM5436907 SRX11410630 GSM5436908 SRP327939 SRS9454265 GSM5436908 RNA-Seq TRANSCRIPTOMIC cDNA Cells were grown overnight, then normalized to OD 0.06 and let grow to OD 0.5 and 0.8. Cells were pelleted and snap frozen for RNA extraction with the Directzol RNA mini-prep plus kit (Zymo Research, R2071). The samples were also DNase treated with TURBO DNase (Thermo Fisher Scientific, AM2239), rRNA was removed with Rybo-off rRNA depletion kit (Vazyme Biotech, N407-01) and then sequenced with NovaSeq 6000 (Illumina: flow cell S1, 100 cycles) using a 50 bp paired-end read. NEBNext Ultra II Directional RNA Illumina NovaSeq 6000 gds 305436908 GSM5436908 GEO Accession GSM5436908 SRX11410631 GSM5436909 SRP327939 SRS9454266 GSM5436909 RNA-Seq TRANSCRIPTOMIC cDNA Cells were grown overnight, then normalized to OD 0.06 and let grow to OD 0.5 and 0.8. Cells were pelleted and snap frozen for RNA extraction with the Directzol RNA mini-prep plus kit (Zymo Research, R2071). The samples were also DNase treated with TURBO DNase (Thermo Fisher Scientific, AM2239), rRNA was removed with Rybo-off rRNA depletion kit (Vazyme Biotech, N407-01) and then sequenced with NovaSeq 6000 (Illumina: flow cell S1, 100 cycles) using a 50 bp paired-end read. NEBNext Ultra II Directional RNA Illumina NovaSeq 6000 gds 305436909 GSM5436909 GEO Accession GSM5436909 SRX11410632 GSM5436910 SRP327939 SRS9454267 GSM5436910 RNA-Seq TRANSCRIPTOMIC cDNA Cells were grown overnight, then normalized to OD 0.06 and let grow to OD 0.5 and 0.8. Cells were pelleted and snap frozen for RNA extraction with the Directzol RNA mini-prep plus kit (Zymo Research, R2071). The samples were also DNase treated with TURBO DNase (Thermo Fisher Scientific, AM2239), rRNA was removed with Rybo-off rRNA depletion kit (Vazyme Biotech, N407-01) and then sequenced with NovaSeq 6000 (Illumina: flow cell S1, 100 cycles) using a 50 bp paired-end read. NEBNext Ultra II Directional RNA Illumina NovaSeq 6000 gds 305436910 GSM5436910 GEO Accession GSM5436910