SRX11474032 SpCas9_MISER_s4_naive SRP328563 bp0 The MISER library is theoretically composed of all possible N- and C-terminal fragments, including both duplications and deletions. To isolate deletions in a particular size range, the MISER library was digested with BsaI, to excise the dCas9 gene from the vector backbone and run on an agarose gel. Various slices of the MISER library were individually gel extracted, ligated into expression vector pSAH063, and transformed into E. coli. SRS9514527 SpCas9 MISER Slice 4 Naive SpCas9_MISER_s4_naive Tn-Seq SYNTHETIC other Illumina HiSeq 4000 SRX11474033 SpCas9_MISER_s4_sort SRP328563 bp0 The MISER library is theoretically composed of all possible N- and C-terminal fragments, including both duplications and deletions. To isolate deletions in a particular size range, the MISER library was digested with BsaI, to excise the dCas9 gene from the vector backbone and run on an agarose gel. Various slices of the MISER library were individually gel extracted, ligated into expression vector pSAH063, and transformed into E. coli. SRS9514529 SpCas9 MISER Slice 4 Sort SpCas9_MISER_s4_sort Tn-Seq SYNTHETIC other Illumina HiSeq 4000 SRX11474034 SpCas9_MISER_s5_naive SRP328563 bp0 The MISER library is theoretically composed of all possible N- and C-terminal fragments, including both duplications and deletions. To isolate deletions in a particular size range, the MISER library was digested with BsaI, to excise the dCas9 gene from the vector backbone and run on an agarose gel. Various slices of the MISER library were individually gel extracted, ligated into expression vector pSAH063, and transformed into E. coli. SRS9514530 SpCas9 MISER Slice 5 Naive SpCas9_MISER_s5_naive Tn-Seq SYNTHETIC other Illumina HiSeq 4000 SRX11474035 SpCas9_MISER_s5_sort SRP328563 bp0 The MISER library is theoretically composed of all possible N- and C-terminal fragments, including both duplications and deletions. To isolate deletions in a particular size range, the MISER library was digested with BsaI, to excise the dCas9 gene from the vector backbone and run on an agarose gel. Various slices of the MISER library were individually gel extracted, ligated into expression vector pSAH063, and transformed into E. coli. SRS9514528 SpCas9 MISER Slice 5 Sort SpCas9_MISER_s5_sort Tn-Seq SYNTHETIC other Illumina HiSeq 4000