SRX11481998 29113_L1_1 SRP328661 bp0 Individual PCR reactions were performed per amplicon under non-saturating conditions, before purification using AMPure XP beads and validated using the fragment Analyzer (High Sensitivity Small Fragment Analysis Kit). The amplicons were then normalised individually at 0.54nM concentration and pooled into equal volumes. A second PCR step attached the dual indices and Illumina sequencing adapters using the Nextera XT Index kit, with a final AMPure XP beads purification step, with validation and quantification of the final libraries. Each library was sequenced using an Illumina MiSeq 2 with 2 x 250 bp v2 paired end run SRS9521906 29113_CRISPRh x 29113_hdrGFP L1_1 29113_L1_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX11482000 29113_L1_2 SRP328661 bp0 Individual PCR reactions were performed per amplicon under non-saturating conditions, before purification using AMPure XP beads and validated using the fragment Analyzer (High Sensitivity Small Fragment Analysis Kit). The amplicons were then normalised individually at 0.54nM concentration and pooled into equal volumes. A second PCR step attached the dual indices and Illumina sequencing adapters using the Nextera XT Index kit, with a final AMPure XP beads purification step, with validation and quantification of the final libraries. Each library was sequenced using an Illumina MiSeq 2 with 2 x 250 bp v2 paired end run SRS9521904 29113_CRISPRh x 29113_hdrGFP L1_2 29113_L1_2 AMPLICON GENOMIC PCR Illumina MiSeq SRX11482002 29113_adults SRP328661 bp0 Individual PCR reactions were performed per amplicon under non-saturating conditions, before purification using AMPure XP beads and validated using the fragment Analyzer (High Sensitivity Small Fragment Analysis Kit). The amplicons were then normalised individually at 0.54nM concentration and pooled into equal volumes. A second PCR step attached the dual indices and Illumina sequencing adapters using the Nextera XT Index kit, with a final AMPure XP beads purification step, with validation and quantification of the final libraries. Each library was sequenced using an Illumina MiSeq 2 with 2 x 250 bp v2 paired end run SRS9521907 29113_CRISPRh x 29113_hdrGFP adults 29113_adults AMPLICON GENOMIC PCR Illumina MiSeq SRX11482003 29113_G7 SRP328661 bp0 Individual PCR reactions were performed per amplicon under non-saturating conditions, before purification using AMPure XP beads and validated using the fragment Analyzer (High Sensitivity Small Fragment Analysis Kit). The amplicons were then normalised individually at 0.54nM concentration and pooled into equal volumes. A second PCR step attached the dual indices and Illumina sequencing adapters using the Nextera XT Index kit, with a final AMPure XP beads purification step, with validation and quantification of the final libraries. Each library was sequenced using an Illumina MiSeq 2 with 2 x 250 bp v2 paired end run SRS9521909 29113_CRISPRh x 29113_CRISPRh G7 29113_G7 AMPLICON GENOMIC PCR Illumina MiSeq SRX11482004 29113_Wt SRP328661 bp0 Individual PCR reactions were performed per amplicon under non-saturating conditions, before being sequenced using an Illumina MiSeq 2 by Genewiz SRS9521905 29113_Wt 29113_Wt AMPLICON GENOMIC PCR Illumina MiSeq