SRX11500221 GSM5462325 SRP328976 SRS9539171 GSM5462325 RIP-Seq TRANSCRIPTOMIC other The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT) 25-61005through two rounds of purification. Then the captured RNA was fragmented into small pieces using the Magnesium RNA Fragmentation Module for 7 min at 86°C. The fragmented RNA was premixed with the Dynabeads Antibody Coupling Kit and m6A antibody（No.202003, Synaptic Systems, Germany） in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630) and incubated for 2 h at 4°C. The IP RNA was reverse-transcribed to synthesize the cDNA using the SuperScript™ II Reverse Transcriptase. Then the two-strand synthesis was performed with RNase H using the E. coli DNA polymerase I to synthesize U-labeled second-stranded DNAs. At the same time, the dUTP Solution was incorporated into the two strands, and the ends of the double-stranded DNA were filled with blunt ends. An A-base was added at each end of the fragment to connect it to the T-base overhang, and the size of the fragment was screened and purified using the AMPureXP beads. The U-labeled second-stranded DNAs were digested using a heat-labile UDG enzyme , then amplified by PCR under the following conditions: Pre-denaturation at 95°C for 3 min, denaturation at 98°C (8 cycles) for 15 s, annealing at 60°C for 15 s, extending at 72°C for 30 s, and finally extending at 72°C for 5 min to form a cDNA library of 300 ± 50 BP. At last, the 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™ 6000. Illumina NovaSeq 6000 gds 305462325 GSM5462325 GEO Accession GSM5462325 SRX11500222 GSM5462326 SRP328976 SRS9539172 GSM5462326 RIP-Seq TRANSCRIPTOMIC other The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT) 25-61005through two rounds of purification. Then the captured RNA was fragmented into small pieces using the Magnesium RNA Fragmentation Module for 7 min at 86°C. The fragmented RNA was premixed with the Dynabeads Antibody Coupling Kit and m6A antibody（No.202003, Synaptic Systems, Germany） in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630) and incubated for 2 h at 4°C. The IP RNA was reverse-transcribed to synthesize the cDNA using the SuperScript™ II Reverse Transcriptase. Then the two-strand synthesis was performed with RNase H using the E. coli DNA polymerase I to synthesize U-labeled second-stranded DNAs. At the same time, the dUTP Solution was incorporated into the two strands, and the ends of the double-stranded DNA were filled with blunt ends. An A-base was added at each end of the fragment to connect it to the T-base overhang, and the size of the fragment was screened and purified using the AMPureXP beads. The U-labeled second-stranded DNAs were digested using a heat-labile UDG enzyme , then amplified by PCR under the following conditions: Pre-denaturation at 95°C for 3 min, denaturation at 98°C (8 cycles) for 15 s, annealing at 60°C for 15 s, extending at 72°C for 30 s, and finally extending at 72°C for 5 min to form a cDNA library of 300 ± 50 BP. At last, the 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™ 6000. Illumina NovaSeq 6000 gds 305462326 GSM5462326 GEO Accession GSM5462326 SRX11500223 GSM5462327 SRP328976 SRS9539173 GSM5462327 RIP-Seq TRANSCRIPTOMIC other The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT) 25-61005through two rounds of purification. Then the captured RNA was fragmented into small pieces using the Magnesium RNA Fragmentation Module for 7 min at 86°C. The fragmented RNA was premixed with the Dynabeads Antibody Coupling Kit and m6A antibody（No.202003, Synaptic Systems, Germany） in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630) and incubated for 2 h at 4°C. The IP RNA was reverse-transcribed to synthesize the cDNA using the SuperScript™ II Reverse Transcriptase. Then the two-strand synthesis was performed with RNase H using the E. coli DNA polymerase I to synthesize U-labeled second-stranded DNAs. At the same time, the dUTP Solution was incorporated into the two strands, and the ends of the double-stranded DNA were filled with blunt ends. An A-base was added at each end of the fragment to connect it to the T-base overhang, and the size of the fragment was screened and purified using the AMPureXP beads. The U-labeled second-stranded DNAs were digested using a heat-labile UDG enzyme , then amplified by PCR under the following conditions: Pre-denaturation at 95°C for 3 min, denaturation at 98°C (8 cycles) for 15 s, annealing at 60°C for 15 s, extending at 72°C for 30 s, and finally extending at 72°C for 5 min to form a cDNA library of 300 ± 50 BP. At last, the 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™ 6000. Illumina NovaSeq 6000 gds 305462327 GSM5462327 GEO Accession GSM5462327 SRX11500224 GSM5462328 SRP328976 SRS9539174 GSM5462328 RIP-Seq TRANSCRIPTOMIC other The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT) 25-61005through two rounds of purification. Then the captured RNA was fragmented into small pieces using the Magnesium RNA Fragmentation Module for 7 min at 86°C. The fragmented RNA was premixed with the Dynabeads Antibody Coupling Kit and m6A antibody（No.202003, Synaptic Systems, Germany） in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630) and incubated for 2 h at 4°C. The IP RNA was reverse-transcribed to synthesize the cDNA using the SuperScript™ II Reverse Transcriptase. Then the two-strand synthesis was performed with RNase H using the E. coli DNA polymerase I to synthesize U-labeled second-stranded DNAs. At the same time, the dUTP Solution was incorporated into the two strands, and the ends of the double-stranded DNA were filled with blunt ends. An A-base was added at each end of the fragment to connect it to the T-base overhang, and the size of the fragment was screened and purified using the AMPureXP beads. The U-labeled second-stranded DNAs were digested using a heat-labile UDG enzyme , then amplified by PCR under the following conditions: Pre-denaturation at 95°C for 3 min, denaturation at 98°C (8 cycles) for 15 s, annealing at 60°C for 15 s, extending at 72°C for 30 s, and finally extending at 72°C for 5 min to form a cDNA library of 300 ± 50 BP. At last, the 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™ 6000. Illumina NovaSeq 6000 gds 305462328 GSM5462328 GEO Accession GSM5462328 SRX11500225 GSM5462329 SRP328976 SRS9539175 GSM5462329 RIP-Seq TRANSCRIPTOMIC other The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT) 25-61005through two rounds of purification. Then the captured RNA was fragmented into small pieces using the Magnesium RNA Fragmentation Module for 7 min at 86°C. The fragmented RNA was premixed with the Dynabeads Antibody Coupling Kit and m6A antibody（No.202003, Synaptic Systems, Germany） in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630) and incubated for 2 h at 4°C. The IP RNA was reverse-transcribed to synthesize the cDNA using the SuperScript™ II Reverse Transcriptase. Then the two-strand synthesis was performed with RNase H using the E. coli DNA polymerase I to synthesize U-labeled second-stranded DNAs. At the same time, the dUTP Solution was incorporated into the two strands, and the ends of the double-stranded DNA were filled with blunt ends. An A-base was added at each end of the fragment to connect it to the T-base overhang, and the size of the fragment was screened and purified using the AMPureXP beads. The U-labeled second-stranded DNAs were digested using a heat-labile UDG enzyme , then amplified by PCR under the following conditions: Pre-denaturation at 95°C for 3 min, denaturation at 98°C (8 cycles) for 15 s, annealing at 60°C for 15 s, extending at 72°C for 30 s, and finally extending at 72°C for 5 min to form a cDNA library of 300 ± 50 BP. At last, the 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™ 6000. Illumina NovaSeq 6000 gds 305462329 GSM5462329 GEO Accession GSM5462329 SRX11500226 GSM5462330 SRP328976 SRS9539176 GSM5462330 RIP-Seq TRANSCRIPTOMIC other The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT) 25-61005through two rounds of purification. Then the captured RNA was fragmented into small pieces using the Magnesium RNA Fragmentation Module for 7 min at 86°C. The fragmented RNA was premixed with the Dynabeads Antibody Coupling Kit and m6A antibody（No.202003, Synaptic Systems, Germany） in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630) and incubated for 2 h at 4°C. The IP RNA was reverse-transcribed to synthesize the cDNA using the SuperScript™ II Reverse Transcriptase. Then the two-strand synthesis was performed with RNase H using the E. coli DNA polymerase I to synthesize U-labeled second-stranded DNAs. At the same time, the dUTP Solution was incorporated into the two strands, and the ends of the double-stranded DNA were filled with blunt ends. An A-base was added at each end of the fragment to connect it to the T-base overhang, and the size of the fragment was screened and purified using the AMPureXP beads. The U-labeled second-stranded DNAs were digested using a heat-labile UDG enzyme , then amplified by PCR under the following conditions: Pre-denaturation at 95°C for 3 min, denaturation at 98°C (8 cycles) for 15 s, annealing at 60°C for 15 s, extending at 72°C for 30 s, and finally extending at 72°C for 5 min to form a cDNA library of 300 ± 50 BP. At last, the 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™ 6000. Illumina NovaSeq 6000 gds 305462330 GSM5462330 GEO Accession GSM5462330 SRX11500227 GSM5462331 SRP328976 SRS9539177 GSM5462331 RIP-Seq TRANSCRIPTOMIC other The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT) 25-61005through two rounds of purification. Then the captured RNA was fragmented into small pieces using the Magnesium RNA Fragmentation Module for 7 min at 86°C. The fragmented RNA was premixed with the Dynabeads Antibody Coupling Kit and m6A antibody（No.202003, Synaptic Systems, Germany） in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630) and incubated for 2 h at 4°C. The IP RNA was reverse-transcribed to synthesize the cDNA using the SuperScript™ II Reverse Transcriptase. Then the two-strand synthesis was performed with RNase H using the E. coli DNA polymerase I to synthesize U-labeled second-stranded DNAs. At the same time, the dUTP Solution was incorporated into the two strands, and the ends of the double-stranded DNA were filled with blunt ends. An A-base was added at each end of the fragment to connect it to the T-base overhang, and the size of the fragment was screened and purified using the AMPureXP beads. The U-labeled second-stranded DNAs were digested using a heat-labile UDG enzyme , then amplified by PCR under the following conditions: Pre-denaturation at 95°C for 3 min, denaturation at 98°C (8 cycles) for 15 s, annealing at 60°C for 15 s, extending at 72°C for 30 s, and finally extending at 72°C for 5 min to form a cDNA library of 300 ± 50 BP. At last, the 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™ 6000. Illumina NovaSeq 6000 gds 305462331 GSM5462331 GEO Accession GSM5462331 SRX11500228 GSM5462332 SRP328976 SRS9539178 GSM5462332 RIP-Seq TRANSCRIPTOMIC other The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT) 25-61005through two rounds of purification. Then the captured RNA was fragmented into small pieces using the Magnesium RNA Fragmentation Module for 7 min at 86°C. The fragmented RNA was premixed with the Dynabeads Antibody Coupling Kit and m6A antibody（No.202003, Synaptic Systems, Germany） in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630) and incubated for 2 h at 4°C. The IP RNA was reverse-transcribed to synthesize the cDNA using the SuperScript™ II Reverse Transcriptase. Then the two-strand synthesis was performed with RNase H using the E. coli DNA polymerase I to synthesize U-labeled second-stranded DNAs. At the same time, the dUTP Solution was incorporated into the two strands, and the ends of the double-stranded DNA were filled with blunt ends. An A-base was added at each end of the fragment to connect it to the T-base overhang, and the size of the fragment was screened and purified using the AMPureXP beads. The U-labeled second-stranded DNAs were digested using a heat-labile UDG enzyme , then amplified by PCR under the following conditions: Pre-denaturation at 95°C for 3 min, denaturation at 98°C (8 cycles) for 15 s, annealing at 60°C for 15 s, extending at 72°C for 30 s, and finally extending at 72°C for 5 min to form a cDNA library of 300 ± 50 BP. At last, the 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™ 6000. Illumina NovaSeq 6000 gds 305462332 GSM5462332 GEO Accession GSM5462332 SRX11500229 GSM5462341 SRP328976 SRS9539179 GSM5462341 RIP-Seq TRANSCRIPTOMIC other The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT) 25-61005through two rounds of purification. Then the captured RNA was fragmented into small pieces using the Magnesium RNA Fragmentation Module for 7 min at 86°C. The fragmented RNA was premixed with the Dynabeads Antibody Coupling Kit and m6A antibody（No.202003, Synaptic Systems, Germany） in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630) and incubated for 2 h at 4°C. The IP RNA was reverse-transcribed to synthesize the cDNA using the SuperScript™ II Reverse Transcriptase. Then the two-strand synthesis was performed with RNase H using the E. coli DNA polymerase I to synthesize U-labeled second-stranded DNAs. At the same time, the dUTP Solution was incorporated into the two strands, and the ends of the double-stranded DNA were filled with blunt ends. An A-base was added at each end of the fragment to connect it to the T-base overhang, and the size of the fragment was screened and purified using the AMPureXP beads. The U-labeled second-stranded DNAs were digested using a heat-labile UDG enzyme , then amplified by PCR under the following conditions: Pre-denaturation at 95°C for 3 min, denaturation at 98°C (8 cycles) for 15 s, annealing at 60°C for 15 s, extending at 72°C for 30 s, and finally extending at 72°C for 5 min to form a cDNA library of 300 ± 50 BP. At last, the 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™ 6000. Illumina NovaSeq 6000 gds 305462341 GSM5462341 GEO Accession GSM5462341 SRX11500230 GSM5462324 SRP328976 SRS9539180 GSM5462324 RIP-Seq TRANSCRIPTOMIC other The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT) 25-61005through two rounds of purification. Then the captured RNA was fragmented into small pieces using the Magnesium RNA Fragmentation Module for 7 min at 86°C. The fragmented RNA was premixed with the Dynabeads Antibody Coupling Kit and m6A antibody（No.202003, Synaptic Systems, Germany） in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630) and incubated for 2 h at 4°C. The IP RNA was reverse-transcribed to synthesize the cDNA using the SuperScript™ II Reverse Transcriptase. Then the two-strand synthesis was performed with RNase H using the E. coli DNA polymerase I to synthesize U-labeled second-stranded DNAs. At the same time, the dUTP Solution was incorporated into the two strands, and the ends of the double-stranded DNA were filled with blunt ends. An A-base was added at each end of the fragment to connect it to the T-base overhang, and the size of the fragment was screened and purified using the AMPureXP beads. The U-labeled second-stranded DNAs were digested using a heat-labile UDG enzyme , then amplified by PCR under the following conditions: Pre-denaturation at 95°C for 3 min, denaturation at 98°C (8 cycles) for 15 s, annealing at 60°C for 15 s, extending at 72°C for 30 s, and finally extending at 72°C for 5 min to form a cDNA library of 300 ± 50 BP. At last, the 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™ 6000. Illumina NovaSeq 6000 gds 305462324 GSM5462324 GEO Accession GSM5462324 SRX11500231 GSM5462333 SRP328976 SRS9539181 GSM5462333 RIP-Seq TRANSCRIPTOMIC other The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT) 25-61005through two rounds of purification. Then the captured RNA was fragmented into small pieces using the Magnesium RNA Fragmentation Module for 7 min at 86°C. The fragmented RNA was premixed with the Dynabeads Antibody Coupling Kit and m6A antibody（No.202003, Synaptic Systems, Germany） in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630) and incubated for 2 h at 4°C. The IP RNA was reverse-transcribed to synthesize the cDNA using the SuperScript™ II Reverse Transcriptase. Then the two-strand synthesis was performed with RNase H using the E. coli DNA polymerase I to synthesize U-labeled second-stranded DNAs. At the same time, the dUTP Solution was incorporated into the two strands, and the ends of the double-stranded DNA were filled with blunt ends. An A-base was added at each end of the fragment to connect it to the T-base overhang, and the size of the fragment was screened and purified using the AMPureXP beads. The U-labeled second-stranded DNAs were digested using a heat-labile UDG enzyme , then amplified by PCR under the following conditions: Pre-denaturation at 95°C for 3 min, denaturation at 98°C (8 cycles) for 15 s, annealing at 60°C for 15 s, extending at 72°C for 30 s, and finally extending at 72°C for 5 min to form a cDNA library of 300 ± 50 BP. At last, the 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™ 6000. Illumina NovaSeq 6000 gds 305462333 GSM5462333 GEO Accession GSM5462333 SRX11500232 GSM5462334 SRP328976 SRS9539182 GSM5462334 RIP-Seq TRANSCRIPTOMIC other The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT) 25-61005through two rounds of purification. Then the captured RNA was fragmented into small pieces using the Magnesium RNA Fragmentation Module for 7 min at 86°C. The fragmented RNA was premixed with the Dynabeads Antibody Coupling Kit and m6A antibody（No.202003, Synaptic Systems, Germany） in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630) and incubated for 2 h at 4°C. The IP RNA was reverse-transcribed to synthesize the cDNA using the SuperScript™ II Reverse Transcriptase. Then the two-strand synthesis was performed with RNase H using the E. coli DNA polymerase I to synthesize U-labeled second-stranded DNAs. At the same time, the dUTP Solution was incorporated into the two strands, and the ends of the double-stranded DNA were filled with blunt ends. An A-base was added at each end of the fragment to connect it to the T-base overhang, and the size of the fragment was screened and purified using the AMPureXP beads. The U-labeled second-stranded DNAs were digested using a heat-labile UDG enzyme , then amplified by PCR under the following conditions: Pre-denaturation at 95°C for 3 min, denaturation at 98°C (8 cycles) for 15 s, annealing at 60°C for 15 s, extending at 72°C for 30 s, and finally extending at 72°C for 5 min to form a cDNA library of 300 ± 50 BP. At last, the 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™ 6000. Illumina NovaSeq 6000 gds 305462334 GSM5462334 GEO Accession GSM5462334 SRX11500233 GSM5462335 SRP328976 SRS9539183 GSM5462335 RIP-Seq TRANSCRIPTOMIC other The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT) 25-61005through two rounds of purification. Then the captured RNA was fragmented into small pieces using the Magnesium RNA Fragmentation Module for 7 min at 86°C. The fragmented RNA was premixed with the Dynabeads Antibody Coupling Kit and m6A antibody（No.202003, Synaptic Systems, Germany） in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630) and incubated for 2 h at 4°C. The IP RNA was reverse-transcribed to synthesize the cDNA using the SuperScript™ II Reverse Transcriptase. Then the two-strand synthesis was performed with RNase H using the E. coli DNA polymerase I to synthesize U-labeled second-stranded DNAs. At the same time, the dUTP Solution was incorporated into the two strands, and the ends of the double-stranded DNA were filled with blunt ends. An A-base was added at each end of the fragment to connect it to the T-base overhang, and the size of the fragment was screened and purified using the AMPureXP beads. The U-labeled second-stranded DNAs were digested using a heat-labile UDG enzyme , then amplified by PCR under the following conditions: Pre-denaturation at 95°C for 3 min, denaturation at 98°C (8 cycles) for 15 s, annealing at 60°C for 15 s, extending at 72°C for 30 s, and finally extending at 72°C for 5 min to form a cDNA library of 300 ± 50 BP. At last, the 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™ 6000. Illumina NovaSeq 6000 gds 305462335 GSM5462335 GEO Accession GSM5462335 SRX11500234 GSM5462336 SRP328976 SRS9539184 GSM5462336 RIP-Seq TRANSCRIPTOMIC other The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT) 25-61005through two rounds of purification. Then the captured RNA was fragmented into small pieces using the Magnesium RNA Fragmentation Module for 7 min at 86°C. The fragmented RNA was premixed with the Dynabeads Antibody Coupling Kit and m6A antibody（No.202003, Synaptic Systems, Germany） in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630) and incubated for 2 h at 4°C. The IP RNA was reverse-transcribed to synthesize the cDNA using the SuperScript™ II Reverse Transcriptase. Then the two-strand synthesis was performed with RNase H using the E. coli DNA polymerase I to synthesize U-labeled second-stranded DNAs. At the same time, the dUTP Solution was incorporated into the two strands, and the ends of the double-stranded DNA were filled with blunt ends. An A-base was added at each end of the fragment to connect it to the T-base overhang, and the size of the fragment was screened and purified using the AMPureXP beads. The U-labeled second-stranded DNAs were digested using a heat-labile UDG enzyme , then amplified by PCR under the following conditions: Pre-denaturation at 95°C for 3 min, denaturation at 98°C (8 cycles) for 15 s, annealing at 60°C for 15 s, extending at 72°C for 30 s, and finally extending at 72°C for 5 min to form a cDNA library of 300 ± 50 BP. At last, the 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™ 6000. Illumina NovaSeq 6000 gds 305462336 GSM5462336 GEO Accession GSM5462336 SRX11500235 GSM5462337 SRP328976 SRS9539185 GSM5462337 RIP-Seq TRANSCRIPTOMIC other The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT) 25-61005through two rounds of purification. Then the captured RNA was fragmented into small pieces using the Magnesium RNA Fragmentation Module for 7 min at 86°C. The fragmented RNA was premixed with the Dynabeads Antibody Coupling Kit and m6A antibody（No.202003, Synaptic Systems, Germany） in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630) and incubated for 2 h at 4°C. The IP RNA was reverse-transcribed to synthesize the cDNA using the SuperScript™ II Reverse Transcriptase. Then the two-strand synthesis was performed with RNase H using the E. coli DNA polymerase I to synthesize U-labeled second-stranded DNAs. At the same time, the dUTP Solution was incorporated into the two strands, and the ends of the double-stranded DNA were filled with blunt ends. An A-base was added at each end of the fragment to connect it to the T-base overhang, and the size of the fragment was screened and purified using the AMPureXP beads. The U-labeled second-stranded DNAs were digested using a heat-labile UDG enzyme , then amplified by PCR under the following conditions: Pre-denaturation at 95°C for 3 min, denaturation at 98°C (8 cycles) for 15 s, annealing at 60°C for 15 s, extending at 72°C for 30 s, and finally extending at 72°C for 5 min to form a cDNA library of 300 ± 50 BP. At last, the 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™ 6000. Illumina NovaSeq 6000 gds 305462337 GSM5462337 GEO Accession GSM5462337 SRX11500236 GSM5462338 SRP328976 SRS9539186 GSM5462338 RIP-Seq TRANSCRIPTOMIC other The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT) 25-61005through two rounds of purification. Then the captured RNA was fragmented into small pieces using the Magnesium RNA Fragmentation Module for 7 min at 86°C. The fragmented RNA was premixed with the Dynabeads Antibody Coupling Kit and m6A antibody（No.202003, Synaptic Systems, Germany） in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630) and incubated for 2 h at 4°C. The IP RNA was reverse-transcribed to synthesize the cDNA using the SuperScript™ II Reverse Transcriptase. Then the two-strand synthesis was performed with RNase H using the E. coli DNA polymerase I to synthesize U-labeled second-stranded DNAs. At the same time, the dUTP Solution was incorporated into the two strands, and the ends of the double-stranded DNA were filled with blunt ends. An A-base was added at each end of the fragment to connect it to the T-base overhang, and the size of the fragment was screened and purified using the AMPureXP beads. The U-labeled second-stranded DNAs were digested using a heat-labile UDG enzyme , then amplified by PCR under the following conditions: Pre-denaturation at 95°C for 3 min, denaturation at 98°C (8 cycles) for 15 s, annealing at 60°C for 15 s, extending at 72°C for 30 s, and finally extending at 72°C for 5 min to form a cDNA library of 300 ± 50 BP. At last, the 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™ 6000. Illumina NovaSeq 6000 gds 305462338 GSM5462338 GEO Accession GSM5462338 SRX11500237 GSM5462339 SRP328976 SRS9539187 GSM5462339 RIP-Seq TRANSCRIPTOMIC other The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT) 25-61005through two rounds of purification. Then the captured RNA was fragmented into small pieces using the Magnesium RNA Fragmentation Module for 7 min at 86°C. The fragmented RNA was premixed with the Dynabeads Antibody Coupling Kit and m6A antibody（No.202003, Synaptic Systems, Germany） in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630) and incubated for 2 h at 4°C. The IP RNA was reverse-transcribed to synthesize the cDNA using the SuperScript™ II Reverse Transcriptase. Then the two-strand synthesis was performed with RNase H using the E. coli DNA polymerase I to synthesize U-labeled second-stranded DNAs. At the same time, the dUTP Solution was incorporated into the two strands, and the ends of the double-stranded DNA were filled with blunt ends. An A-base was added at each end of the fragment to connect it to the T-base overhang, and the size of the fragment was screened and purified using the AMPureXP beads. The U-labeled second-stranded DNAs were digested using a heat-labile UDG enzyme , then amplified by PCR under the following conditions: Pre-denaturation at 95°C for 3 min, denaturation at 98°C (8 cycles) for 15 s, annealing at 60°C for 15 s, extending at 72°C for 30 s, and finally extending at 72°C for 5 min to form a cDNA library of 300 ± 50 BP. At last, the 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™ 6000. Illumina NovaSeq 6000 gds 305462339 GSM5462339 GEO Accession GSM5462339 SRX11500238 GSM5462340 SRP328976 SRS9539188 GSM5462340 RIP-Seq TRANSCRIPTOMIC other The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The Poly (A) RNA was purified from 50 μg total RNA using Dynabeads Oligo (dT) 25-61005through two rounds of purification. Then the captured RNA was fragmented into small pieces using the Magnesium RNA Fragmentation Module for 7 min at 86°C. The fragmented RNA was premixed with the Dynabeads Antibody Coupling Kit and m6A antibody（No.202003, Synaptic Systems, Germany） in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630) and incubated for 2 h at 4°C. The IP RNA was reverse-transcribed to synthesize the cDNA using the SuperScript™ II Reverse Transcriptase. Then the two-strand synthesis was performed with RNase H using the E. coli DNA polymerase I to synthesize U-labeled second-stranded DNAs. At the same time, the dUTP Solution was incorporated into the two strands, and the ends of the double-stranded DNA were filled with blunt ends. An A-base was added at each end of the fragment to connect it to the T-base overhang, and the size of the fragment was screened and purified using the AMPureXP beads. The U-labeled second-stranded DNAs were digested using a heat-labile UDG enzyme , then amplified by PCR under the following conditions: Pre-denaturation at 95°C for 3 min, denaturation at 98°C (8 cycles) for 15 s, annealing at 60°C for 15 s, extending at 72°C for 30 s, and finally extending at 72°C for 5 min to form a cDNA library of 300 ± 50 BP. At last, the 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™ 6000. Illumina NovaSeq 6000 gds 305462340 GSM5462340 GEO Accession GSM5462340