SRX11501587 GSM5462517 SRP329005 SRS9540510 GSM5462517 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462517 GSM5462517 GEO Accession GSM5462517 SRX11501588 GSM5462518 SRP329005 SRS9540511 GSM5462518 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462518 GSM5462518 GEO Accession GSM5462518 SRX11501589 GSM5462519 SRP329005 SRS9540512 GSM5462519 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462519 GSM5462519 GEO Accession GSM5462519 SRX11501590 GSM5462520 SRP329005 SRS9540513 GSM5462520 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462520 GSM5462520 GEO Accession GSM5462520 SRX11501591 GSM5462521 SRP329005 SRS9540514 GSM5462521 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462521 GSM5462521 GEO Accession GSM5462521 SRX11501592 GSM5462522 SRP329005 SRS9540515 GSM5462522 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462522 GSM5462522 GEO Accession GSM5462522 SRX11501593 GSM5462523 SRP329005 SRS9540516 GSM5462523 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462523 GSM5462523 GEO Accession GSM5462523 SRX11501594 GSM5462524 SRP329005 SRS9540517 GSM5462524 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462524 GSM5462524 GEO Accession GSM5462524 SRX11501595 GSM5462525 SRP329005 SRS9540518 GSM5462525 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462525 GSM5462525 GEO Accession GSM5462525 SRX11501596 GSM5462526 SRP329005 SRS9540519 GSM5462526 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462526 GSM5462526 GEO Accession GSM5462526 SRX11501597 GSM5462527 SRP329005 SRS9540520 GSM5462527 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462527 GSM5462527 GEO Accession GSM5462527 SRX11501598 GSM5462528 SRP329005 SRS9540521 GSM5462528 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462528 GSM5462528 GEO Accession GSM5462528 SRX11501599 GSM5462529 SRP329005 SRS9540522 GSM5462529 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462529 GSM5462529 GEO Accession GSM5462529 SRX11501600 GSM5462530 SRP329005 SRS9540523 GSM5462530 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462530 GSM5462530 GEO Accession GSM5462530 SRX11501601 GSM5462531 SRP329005 SRS9540524 GSM5462531 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462531 GSM5462531 GEO Accession GSM5462531 SRX11501602 GSM5462532 SRP329005 SRS9540525 GSM5462532 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462532 GSM5462532 GEO Accession GSM5462532 SRX11501603 GSM5462533 SRP329005 SRS9540526 GSM5462533 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462533 GSM5462533 GEO Accession GSM5462533 SRX11501604 GSM5462534 SRP329005 SRS9540527 GSM5462534 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462534 GSM5462534 GEO Accession GSM5462534 SRX11501605 GSM5462535 SRP329005 SRS9540528 GSM5462535 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462535 GSM5462535 GEO Accession GSM5462535 SRX11501606 GSM5462536 SRP329005 SRS9540529 GSM5462536 RNA-Seq TRANSCRIPTOMIC cDNA Euthanized mice were perfused with chilled 1x PBS via the left ventricle. Kidneys were harvested, minced into approximately 1 mm3 cubes and digested using Multi Tissue dissociation kit (Miltenyi, 130-110-201). The tissue was homogenized using 21G and 26.5G syringes. Up to 0.25 g of the tissue was digested with 50 µl of Enzyme D, 25 µl of Enzyme R and 6.75 µl of Enzyme A in 1 ml of RPMI and incubated for 30 min at 37 °C. Reaction was deactivated by 10% FBS. The solution was then passed through a 40 µm cell strainer. After centrifugation at 400 g for 5 min, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 min. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227). This method generated single-cell suspensions with >80% viability. Libraries were generated using Chromium Single Cell 3' Reagent Kits v3 (10X Genomics, PN-1000092) and Chromium i7 Multiplex Kit (10X Genomics, PN-120262) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis. Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). Illumina HiSeq 4000 gds 305462536 GSM5462536 GEO Accession GSM5462536