SRP329374 PRJNA748866 Endometrial luminal epithelium as key player during days 10 and 13 of pregnancy in the mare: A spatiotemporal transcriptomics study During the period of maternal recognition of pregnancy (MRP) in the mare, the embryo has to prevent regression of the corpus luteum for successful establishment of pregnancy. This is achieved by mechanical stimuli and release of currently unknown signaling molecules by the migrating embryo. We hypothesized that endometrial gene expression changes in response to the embryos stimuli are highly cell type-specific. To determine the specificity of this response, a spatiotemporal transcriptomics approach combining laser capture microdissection of luminal and glandular epithelium (LE, GE), and stroma (ST) of biopsy samples collected from days 10 and 13 of pregnancy (P) and the estrous cycle (C) together with low-input-RNA sequencing was applied. This study showed that major endometrial changes towards the embryo occur in the LE (1251 DEGs) between P and C (regardless the day) compared to GE (248) and in ST (103). pubmed 34785745