SRX11519425 GMN1_MF1 SRP329345 PRJNA748817 S-D-Bact-0341-b-S-17 and S-D-Bact-0785-a-A-21 primers with 5' Illumina adapter overhangs (underlined) (5'-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3 and 5'-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3, respectively) were used for obtaining amplicons corresponding to hypervariable V3-V4 region using the 16S metagenomic sequencing library preparation kit (Illumina, San Diego, CA, USA). Indexed adapters were incorporated into the amplicons through Nextera XT Index Kit (Illumina, San Diego, CA, USA) as per the manufacturers instructions. The amplicons sizing accuracy was screened through the 4200 TapeStation System (Agilent Technologies, Santa Clara, CA, USA). SRS9557126 Green manure: maize flowering 1 (GMN1_MF1) GMN1_MF1 AMPLICON METAGENOMIC PCR Illumina MiSeq