SRX11528714 GSM5468070 SRP329588 SRS9565992 GSM5468070 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468070 GSM5468070 GEO Accession GSM5468070 SRX11528715 GSM5468071 SRP329588 SRS9565993 GSM5468071 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468071 GSM5468071 GEO Accession GSM5468071 SRX11528716 GSM5468072 SRP329588 SRS9565994 GSM5468072 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468072 GSM5468072 GEO Accession GSM5468072 SRX11528717 GSM5468073 SRP329588 SRS9565995 GSM5468073 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468073 GSM5468073 GEO Accession GSM5468073 SRX11528718 GSM5468074 SRP329588 SRS9565996 GSM5468074 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468074 GSM5468074 GEO Accession GSM5468074 SRX11528719 GSM5468075 SRP329588 SRS9565997 GSM5468075 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468075 GSM5468075 GEO Accession GSM5468075 SRX11528720 GSM5468076 SRP329588 SRS9565998 GSM5468076 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468076 GSM5468076 GEO Accession GSM5468076 SRX11528721 GSM5468077 SRP329588 SRS9565999 GSM5468077 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468077 GSM5468077 GEO Accession GSM5468077 SRX11528722 GSM5468078 SRP329588 SRS9566000 GSM5468078 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468078 GSM5468078 GEO Accession GSM5468078 SRX11528723 GSM5468079 SRP329588 SRS9566001 GSM5468079 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468079 GSM5468079 GEO Accession GSM5468079 SRX11528724 GSM5468080 SRP329588 SRS9566002 GSM5468080 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468080 GSM5468080 GEO Accession GSM5468080 SRX11528725 GSM5468081 SRP329588 SRS9566003 GSM5468081 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468081 GSM5468081 GEO Accession GSM5468081 SRX11528726 GSM5468082 SRP329588 SRS9566004 GSM5468082 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468082 GSM5468082 GEO Accession GSM5468082 SRX11528727 GSM5468083 SRP329588 SRS9566005 GSM5468083 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468083 GSM5468083 GEO Accession GSM5468083 SRX11528728 GSM5468084 SRP329588 SRS9566006 GSM5468084 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468084 GSM5468084 GEO Accession GSM5468084 SRX11528729 GSM5468085 SRP329588 SRS9566007 GSM5468085 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468085 GSM5468085 GEO Accession GSM5468085 SRX11528730 GSM5468086 SRP329588 SRS9566008 GSM5468086 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468086 GSM5468086 GEO Accession GSM5468086 SRX11528731 GSM5468087 SRP329588 SRS9566009 GSM5468087 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468087 GSM5468087 GEO Accession GSM5468087 SRX11528732 GSM5468088 SRP329588 SRS9566010 GSM5468088 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468088 GSM5468088 GEO Accession GSM5468088 SRX11528733 GSM5468089 SRP329588 SRS9566011 GSM5468089 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468089 GSM5468089 GEO Accession GSM5468089 SRX11528734 GSM5468090 SRP329588 SRS9566012 GSM5468090 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468090 GSM5468090 GEO Accession GSM5468090 SRX11528735 GSM5468091 SRP329588 SRS9566013 GSM5468091 ChIP-Seq GENOMIC ChIP Cells were fixed in the plates with 1% formaldehyde. Each 5 million cells were resuspended in 1 mL of hypotonic lysis buffer (20mM TRIS HCl pH8.0, 85mM KCl, 0.5% IGEPAL, 1X HALT protease inhibitor) and incubated 15 min on ice. Cells were then spun down 5 min, 500g, 4C, supernatant was removed and 5 million cells were resuspended in 300 uL of sonication buffer (20mM TRIS HCl pH8.0, 0.2% SDS, 0.5% Sodium Deoxycholate, 1X HALT protease inhibitor). Cells were sonicated using a bioruptor pico, at 4C, following the manufacturer instructions and with the following settings: 30 s on, 30 s off, for 15 cycles. 300 uL of chromatin from 5M cells was diluted with 1 200 uL of dilution IP buffer (20 mM TRIS HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% TRITON, 1X HALT protease inhibitor) and specific antibody was added. For end repair, 35 μL of sample were transferred to a PCR tube, then 15 μL of the end-repair mix (1X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix, 7.5 U T4 DNA polymerase (NEBM0203L), 25 U T4 polynucleotide kinase (NEB M0201S), 2.5 U Klenow polymerase Polymerase I (NEB M0210L)) were added. The reactions were then incubated at 37°C for 30 minutes, followed by incubation at 75°C for 20 minutes to inactivate Klenow polymerase. An ampure beads purification was then performed (1.6X) and DNA was eluted in 41 uL of water. For A-tailing, dATP was added to the 3' ends by adding 9 μL of A-tailing mix (5 μL NEB buffer 2.1, 1 μL of 1 mM dATP, 5 U Klenow exo (NEB M0212S)) to the 41 μL of DNA sample from the previous step. The reaction was incubated in a PCR machine at 37°C for 30 minutes, then at 65°C for 20 minutes. An ampure beads purification was then performed (1.6X) and DNA was eluted in 40 uL of water. The TrueSeq DNA LT kits Set A (Illumina, #15041757) was used for adapter ligation and PCR amplification. PCR primers were removed using Ampure XP beads. The ChIP-seq libraries were sequenced using 50 bp paired end reads on Illumina HiSeq4000. Illumina HiSeq 4000 gds 305468091 GSM5468091 GEO Accession GSM5468091