SRP330481 PRJNA750759 Spliced-Leader sequencing of Trypansoma from cattle blood Trypanosoma vivax is a major causative agent of animal African trypanosomiasis. Here, we used a targeted sequencing approach which enriches for transcripts containing the spliced leader sequence of Trypanosoma spp. to understand gene expression in natural infections. These sequences were obtained from RNA isolated directly from the blood of cattle infected with Trypanosoma parasites.