SRX450247 GSM1314510 SRP035860 GSE54405 SRS543656 GSM1314510 RNA-Seq TRANSCRIPTOMIC cDNA From the cells cultured in M9 minimal media at 30 ℃, total RNA was extracted using RNAsnapTM method, followed by the ethanol precipitation. rRNA was removed using ribo-zeroTM magnetic kit for bacteria in accordance with manufacturer’s instruction (Epicentre). rRNA removal was confirmed using ExperionTM system. Subsequently, 4 µg of the purified RNA was fragmented to sizes of ~300 bp using RNA fragmentation reagent (Ambion, Grand Island, NY). 50 ng of the fragmented RNA was converted to sequencing library using TruSeq® Stranded mRNA Sample Prep Kit in accordance with manufacturer’s instruction (Illumina). Illumina MiSeq GEO Sample GSM1314510 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1314510 gds 301314510 GEO Accession GSM1314510 SRX450248 GSM1314511 SRP035860 GSE54405 SRS543657 GSM1314511 RNA-Seq TRANSCRIPTOMIC cDNA From the cells cultured in M9 minimal media at 30 ℃, total RNA was extracted using RNAsnapTM method, followed by the ethanol precipitation. rRNA was removed using ribo-zeroTM magnetic kit for bacteria in accordance with manufacturer’s instruction (Epicentre). rRNA removal was confirmed using ExperionTM system. Subsequently, 4 µg of the purified RNA was fragmented to sizes of ~300 bp using RNA fragmentation reagent (Ambion, Grand Island, NY). 50 ng of the fragmented RNA was converted to sequencing library using TruSeq® Stranded mRNA Sample Prep Kit in accordance with manufacturer’s instruction (Illumina). Illumina MiSeq GEO Sample GSM1314511 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1314511 gds 301314511 GEO Accession GSM1314511