SRP341267 PRJNA771080 GSE185874 Engineering Extracellular Vesicles by Three-dimensional Dynamic Culture of Human Mesenchymal Stem Cells In current study, hMSCs were grown as 3D aggregates under wave motion to promote EV secretion (3D EVs). mRNA sequencing reveals global transcriptome alterations (e.g., upregulated Wnt, TNF, and Hippo signaling and downregulated cellular senescence) for 3D aggregates. Overall design: MSC cell were cultured under either 2D, 3D or hypoxia conditions, and harvested for mRNA-seq. GSE185874 pubmed 35716062