SRP341447 PRJNA771382 Soil microbiota under N rate and crop rotation treatment, 2015-2016, Illinois This study sought to identify bioindicators of soil health under different N fertilization rates and crop rotation treatments in Illinois Mollisols. N rate treatment included 0, 202, and 269 kg N/ha, and crop rotations were continuous corn and corn-soybean rotation; the soils were managed as such for 35 years since 1981. The plots were arranged in randomized complete block design with three replicates. The soil samples were taken in 2015 and in 2016.