gRNA sequencing of Raji single cell clone 2E6

SRX12629370 SC13 gRNA sequencing of Raji single cell clone 2E6 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC13 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629371 SC14 gRNA sequencing of Raji single cell clone 2G10 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC14 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629372 SC15 gRNA sequencing of Raji single cell clone 4B3 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC15 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629373 SC16 gRNA sequencing of Raji single cell clone 4D2 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC16 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629374 SC17 gRNA sequencing of Raji single cell clone 4D10 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC17 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629375 SC18 gRNA sequencing of Raji single cell clone 4D11 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC18 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629376 SC19 gRNA sequencing of Raji single cell clone 4E8 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC19 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629377 SC20 gRNA sequencing of Raji single cell clone 4F6 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC20 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629378 SC03 gRNA sequencing of Raji single cell clone 1D5 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC03 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629379 SC21 gRNA sequencing of Raji single cell clone 5B10 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC21 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629380 SC22 gRNA sequencing of Raji single cell clone 5D7 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC22 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629381 SC23 gRNA sequencing of Raji single cell clone 5D9 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC23 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629382 SC24 gRNA sequencing of Raji single cell clone 5E11 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC24 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629383 SC04 gRNA sequencing of Raji single cell clone 1D6 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC04 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629384 SC05 gRNA sequencing of Raji single cell clone 1D7 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC05 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629385 SC06 gRNA sequencing of Raji single cell clone 1D8 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC06 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629386 SC07 gRNA sequencing of Raji single cell clone 1G9 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC07 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629387 SC08 gRNA sequencing of Raji single cell clone 2C2 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC08 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629388 SC09 gRNA sequencing of Raji single cell clone 2C5 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC09 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629389 SC10 gRNA sequencing of Raji single cell clone 2C6 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC10 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629390 SC01 gRNA sequencing of Raji single cell clone 1C9 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC01 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629391 SC02 gRNA sequencing of Raji single cell clone 1D3 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC02 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629392 SC11 gRNA sequencing of Raji single cell clone 2D3 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC11 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX12629393 SC12 gRNA sequencing of Raji single cell clone 2E4 SRP341530 PRJNA771240 Raji cells were transduced with a genome-wide CRISPR Cas9 knock-out library and sorted for cells losing soluble TCR binding. From the sorted negative cells, single cell clones were derived, the contained gRNAs amplified via PCR and sequenced via Illumina NGS. SRS10582989 SAMN22263207 SC12 AMPLICON SYNTHETIC PCR Illumina MiSeq