SRX12633262 GSM5628243 SRP341605 SRS10586660 GSM5628243 RNA-Seq TRANSCRIPTOMIC cDNA Tissues that were snap frozen in liquid nitrogen were removed from -80C. Tissues were homogenized in trizol with the bullet blender at 4C. RNA extraction was done with Qiagen RNeasy Kit. Samples were DNAse treated with the ThermoFisher Turbo DNA Free. The concentration and quality of the RNA of the samples was first assesed with the Agilent 2100 BioAnalyzer. An RNA Integrity Number (RIN) of 5 or higher was required to pass the quality control but a RIN of 9 or higher was desired. The 100ng of RNA was used for cDNA library preparation which included mRNA purification/enrichment, RNA fragmentation, cDNA synthesis, ligation of index adaptors, and application following KAPA mRNA Hyperprep Kit prep (KK8581). Each resulting indexed library was quantified and its quality accessed by NovaSeq 6000; 5µL of 2nM pooled libraries per lane were denatured, neutralized, and applied to the cBot for flow cell deposition and cluster amplification before loading to HiSeq 3000/4000 for 75bp paired-end sequencing (Illumnia, Inc). Approximately 50M reads per library were generated. A Phred quality score (Q score) was used to measure the quality of sequencing. More than 90% of the sequencing reads reached Q30 (99.9% base call accuracy). The sequence reads were mapped to the designated reference genome using STAR (Spliced Transcripts Alignment to a Reference). Illumina NovaSeq 6000 gds 305628243 GSM5628243 GEO Accession GSM5628243 SRX12633263 GSM5628244 SRP341605 SRS10586661 GSM5628244 RNA-Seq TRANSCRIPTOMIC cDNA Tissues that were snap frozen in liquid nitrogen were removed from -80C. Tissues were homogenized in trizol with the bullet blender at 4C. RNA extraction was done with Qiagen RNeasy Kit. Samples were DNAse treated with the ThermoFisher Turbo DNA Free. The concentration and quality of the RNA of the samples was first assesed with the Agilent 2100 BioAnalyzer. An RNA Integrity Number (RIN) of 5 or higher was required to pass the quality control but a RIN of 9 or higher was desired. The 100ng of RNA was used for cDNA library preparation which included mRNA purification/enrichment, RNA fragmentation, cDNA synthesis, ligation of index adaptors, and application following KAPA mRNA Hyperprep Kit prep (KK8581). Each resulting indexed library was quantified and its quality accessed by NovaSeq 6000; 5µL of 2nM pooled libraries per lane were denatured, neutralized, and applied to the cBot for flow cell deposition and cluster amplification before loading to HiSeq 3000/4000 for 75bp paired-end sequencing (Illumnia, Inc). Approximately 50M reads per library were generated. A Phred quality score (Q score) was used to measure the quality of sequencing. More than 90% of the sequencing reads reached Q30 (99.9% base call accuracy). The sequence reads were mapped to the designated reference genome using STAR (Spliced Transcripts Alignment to a Reference). Illumina NovaSeq 6000 gds 305628244 GSM5628244 GEO Accession GSM5628244 SRX12633264 GSM5628245 SRP341605 SRS10586662 GSM5628245 RNA-Seq TRANSCRIPTOMIC cDNA Tissues that were snap frozen in liquid nitrogen were removed from -80C. Tissues were homogenized in trizol with the bullet blender at 4C. RNA extraction was done with Qiagen RNeasy Kit. Samples were DNAse treated with the ThermoFisher Turbo DNA Free. The concentration and quality of the RNA of the samples was first assesed with the Agilent 2100 BioAnalyzer. An RNA Integrity Number (RIN) of 5 or higher was required to pass the quality control but a RIN of 9 or higher was desired. The 100ng of RNA was used for cDNA library preparation which included mRNA purification/enrichment, RNA fragmentation, cDNA synthesis, ligation of index adaptors, and application following KAPA mRNA Hyperprep Kit prep (KK8581). Each resulting indexed library was quantified and its quality accessed by NovaSeq 6000; 5µL of 2nM pooled libraries per lane were denatured, neutralized, and applied to the cBot for flow cell deposition and cluster amplification before loading to HiSeq 3000/4000 for 75bp paired-end sequencing (Illumnia, Inc). Approximately 50M reads per library were generated. A Phred quality score (Q score) was used to measure the quality of sequencing. More than 90% of the sequencing reads reached Q30 (99.9% base call accuracy). The sequence reads were mapped to the designated reference genome using STAR (Spliced Transcripts Alignment to a Reference). Illumina NovaSeq 6000 gds 305628245 GSM5628245 GEO Accession GSM5628245 SRX12633265 GSM5628246 SRP341605 SRS10586663 GSM5628246 RNA-Seq TRANSCRIPTOMIC cDNA Tissues that were snap frozen in liquid nitrogen were removed from -80C. Tissues were homogenized in trizol with the bullet blender at 4C. RNA extraction was done with Qiagen RNeasy Kit. Samples were DNAse treated with the ThermoFisher Turbo DNA Free. The concentration and quality of the RNA of the samples was first assesed with the Agilent 2100 BioAnalyzer. An RNA Integrity Number (RIN) of 5 or higher was required to pass the quality control but a RIN of 9 or higher was desired. The 100ng of RNA was used for cDNA library preparation which included mRNA purification/enrichment, RNA fragmentation, cDNA synthesis, ligation of index adaptors, and application following KAPA mRNA Hyperprep Kit prep (KK8581). Each resulting indexed library was quantified and its quality accessed by NovaSeq 6000; 5µL of 2nM pooled libraries per lane were denatured, neutralized, and applied to the cBot for flow cell deposition and cluster amplification before loading to HiSeq 3000/4000 for 75bp paired-end sequencing (Illumnia, Inc). Approximately 50M reads per library were generated. A Phred quality score (Q score) was used to measure the quality of sequencing. More than 90% of the sequencing reads reached Q30 (99.9% base call accuracy). The sequence reads were mapped to the designated reference genome using STAR (Spliced Transcripts Alignment to a Reference). Illumina NovaSeq 6000 gds 305628246 GSM5628246 GEO Accession GSM5628246 SRX12633266 GSM5628247 SRP341605 SRS10586664 GSM5628247 RNA-Seq TRANSCRIPTOMIC cDNA Tissues that were snap frozen in liquid nitrogen were removed from -80C. Tissues were homogenized in trizol with the bullet blender at 4C. RNA extraction was done with Qiagen RNeasy Kit. Samples were DNAse treated with the ThermoFisher Turbo DNA Free. The concentration and quality of the RNA of the samples was first assesed with the Agilent 2100 BioAnalyzer. An RNA Integrity Number (RIN) of 5 or higher was required to pass the quality control but a RIN of 9 or higher was desired. The 100ng of RNA was used for cDNA library preparation which included mRNA purification/enrichment, RNA fragmentation, cDNA synthesis, ligation of index adaptors, and application following KAPA mRNA Hyperprep Kit prep (KK8581). Each resulting indexed library was quantified and its quality accessed by NovaSeq 6000; 5µL of 2nM pooled libraries per lane were denatured, neutralized, and applied to the cBot for flow cell deposition and cluster amplification before loading to HiSeq 3000/4000 for 75bp paired-end sequencing (Illumnia, Inc). Approximately 50M reads per library were generated. A Phred quality score (Q score) was used to measure the quality of sequencing. More than 90% of the sequencing reads reached Q30 (99.9% base call accuracy). The sequence reads were mapped to the designated reference genome using STAR (Spliced Transcripts Alignment to a Reference). Illumina NovaSeq 6000 gds 305628247 GSM5628247 GEO Accession GSM5628247 SRX12633267 GSM5628248 SRP341605 SRS10586665 GSM5628248 RNA-Seq TRANSCRIPTOMIC cDNA Tissues that were snap frozen in liquid nitrogen were removed from -80C. Tissues were homogenized in trizol with the bullet blender at 4C. RNA extraction was done with Qiagen RNeasy Kit. Samples were DNAse treated with the ThermoFisher Turbo DNA Free. The concentration and quality of the RNA of the samples was first assesed with the Agilent 2100 BioAnalyzer. An RNA Integrity Number (RIN) of 5 or higher was required to pass the quality control but a RIN of 9 or higher was desired. The 100ng of RNA was used for cDNA library preparation which included mRNA purification/enrichment, RNA fragmentation, cDNA synthesis, ligation of index adaptors, and application following KAPA mRNA Hyperprep Kit prep (KK8581). Each resulting indexed library was quantified and its quality accessed by NovaSeq 6000; 5µL of 2nM pooled libraries per lane were denatured, neutralized, and applied to the cBot for flow cell deposition and cluster amplification before loading to HiSeq 3000/4000 for 75bp paired-end sequencing (Illumnia, Inc). Approximately 50M reads per library were generated. A Phred quality score (Q score) was used to measure the quality of sequencing. More than 90% of the sequencing reads reached Q30 (99.9% base call accuracy). The sequence reads were mapped to the designated reference genome using STAR (Spliced Transcripts Alignment to a Reference). Illumina NovaSeq 6000 gds 305628248 GSM5628248 GEO Accession GSM5628248 SRX12633268 GSM5628249 SRP341605 SRS10586666 GSM5628249 RNA-Seq TRANSCRIPTOMIC cDNA Tissues that were snap frozen in liquid nitrogen were removed from -80C. Tissues were homogenized in trizol with the bullet blender at 4C. RNA extraction was done with Qiagen RNeasy Kit. Samples were DNAse treated with the ThermoFisher Turbo DNA Free. The concentration and quality of the RNA of the samples was first assesed with the Agilent 2100 BioAnalyzer. An RNA Integrity Number (RIN) of 5 or higher was required to pass the quality control but a RIN of 9 or higher was desired. The 100ng of RNA was used for cDNA library preparation which included mRNA purification/enrichment, RNA fragmentation, cDNA synthesis, ligation of index adaptors, and application following KAPA mRNA Hyperprep Kit prep (KK8581). Each resulting indexed library was quantified and its quality accessed by NovaSeq 6000; 5µL of 2nM pooled libraries per lane were denatured, neutralized, and applied to the cBot for flow cell deposition and cluster amplification before loading to HiSeq 3000/4000 for 75bp paired-end sequencing (Illumnia, Inc). Approximately 50M reads per library were generated. A Phred quality score (Q score) was used to measure the quality of sequencing. More than 90% of the sequencing reads reached Q30 (99.9% base call accuracy). The sequence reads were mapped to the designated reference genome using STAR (Spliced Transcripts Alignment to a Reference). Illumina NovaSeq 6000 gds 305628249 GSM5628249 GEO Accession GSM5628249 SRX12633269 GSM5628250 SRP341605 SRS10586667 GSM5628250 RNA-Seq TRANSCRIPTOMIC cDNA Tissues that were snap frozen in liquid nitrogen were removed from -80C. Tissues were homogenized in trizol with the bullet blender at 4C. RNA extraction was done with Qiagen RNeasy Kit. Samples were DNAse treated with the ThermoFisher Turbo DNA Free. The concentration and quality of the RNA of the samples was first assesed with the Agilent 2100 BioAnalyzer. An RNA Integrity Number (RIN) of 5 or higher was required to pass the quality control but a RIN of 9 or higher was desired. The 100ng of RNA was used for cDNA library preparation which included mRNA purification/enrichment, RNA fragmentation, cDNA synthesis, ligation of index adaptors, and application following KAPA mRNA Hyperprep Kit prep (KK8581). Each resulting indexed library was quantified and its quality accessed by NovaSeq 6000; 5µL of 2nM pooled libraries per lane were denatured, neutralized, and applied to the cBot for flow cell deposition and cluster amplification before loading to HiSeq 3000/4000 for 75bp paired-end sequencing (Illumnia, Inc). Approximately 50M reads per library were generated. A Phred quality score (Q score) was used to measure the quality of sequencing. More than 90% of the sequencing reads reached Q30 (99.9% base call accuracy). The sequence reads were mapped to the designated reference genome using STAR (Spliced Transcripts Alignment to a Reference). Illumina NovaSeq 6000 gds 305628250 GSM5628250 GEO Accession GSM5628250 SRX12633270 GSM5628251 SRP341605 SRS10586668 GSM5628251 RNA-Seq TRANSCRIPTOMIC cDNA Tissues that were snap frozen in liquid nitrogen were removed from -80C. Tissues were homogenized in trizol with the bullet blender at 4C. RNA extraction was done with Qiagen RNeasy Kit. Samples were DNAse treated with the ThermoFisher Turbo DNA Free. The concentration and quality of the RNA of the samples was first assesed with the Agilent 2100 BioAnalyzer. An RNA Integrity Number (RIN) of 5 or higher was required to pass the quality control but a RIN of 9 or higher was desired. The 100ng of RNA was used for cDNA library preparation which included mRNA purification/enrichment, RNA fragmentation, cDNA synthesis, ligation of index adaptors, and application following KAPA mRNA Hyperprep Kit prep (KK8581). Each resulting indexed library was quantified and its quality accessed by NovaSeq 6000; 5µL of 2nM pooled libraries per lane were denatured, neutralized, and applied to the cBot for flow cell deposition and cluster amplification before loading to HiSeq 3000/4000 for 75bp paired-end sequencing (Illumnia, Inc). Approximately 50M reads per library were generated. A Phred quality score (Q score) was used to measure the quality of sequencing. More than 90% of the sequencing reads reached Q30 (99.9% base call accuracy). The sequence reads were mapped to the designated reference genome using STAR (Spliced Transcripts Alignment to a Reference). Illumina NovaSeq 6000 gds 305628251 GSM5628251 GEO Accession GSM5628251