SRX12639526 aquavitas_E1 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592892 aquavitas_E1 aquavitas_E1 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639527 aquavitas_E2 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592893 aquavitas_E2 aquavitas_E2 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639528 aquavitas_E11 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592894 aquavitas_E11 aquavitas_E11 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639529 aquavitas_E128 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592895 aquavitas_E128 aquavitas_E128 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639530 aquavitas_E129 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592896 aquavitas_E129 aquavitas_E129 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639531 aquavitas_E130 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592897 aquavitas_E130 aquavitas_E130 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639532 aquavitas_E131 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592898 aquavitas_E131 aquavitas_E131 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639533 aquavitas_E132 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592899 aquavitas_E132 aquavitas_E132 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639534 aquavitas_E133 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592900 aquavitas_E133 aquavitas_E133 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639535 aquavitas_E134 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592901 aquavitas_E134 aquavitas_E134 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639536 aquavitas_E135 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592902 aquavitas_E135 aquavitas_E135 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639537 aquavitas_E136 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592903 aquavitas_E136 aquavitas_E136 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639538 aquavitas_E137 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592904 aquavitas_E137 aquavitas_E137 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639539 aquavitas_E12 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592905 aquavitas_E12 aquavitas_E12 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639540 aquavitas_E138 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592906 aquavitas_E138 aquavitas_E138 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639541 aquavitas_E13 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592907 aquavitas_E13 aquavitas_E13 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639542 aquavitas_E14 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592908 aquavitas_E14 aquavitas_E14 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639543 aquavitas_E15 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592909 aquavitas_E15 aquavitas_E15 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639544 aquavitas_E16 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592910 aquavitas_E16 aquavitas_E16 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639545 aquavitas_E17 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592911 aquavitas_E17 aquavitas_E17 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639546 aquavitas_E18 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592912 aquavitas_E18 aquavitas_E18 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639547 aquavitas_E19 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592913 aquavitas_E19 aquavitas_E19 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639548 aquavitas_E20 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592914 aquavitas_E20 aquavitas_E20 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639549 aquavitas_E3 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592915 aquavitas_E3 aquavitas_E3 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639550 aquavitas_E21 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592916 aquavitas_E21 aquavitas_E21 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639551 aquavitas_E22 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592917 aquavitas_E22 aquavitas_E22 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639552 aquavitas_E23 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592918 aquavitas_E23 aquavitas_E23 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639553 aquavitas_E24 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592919 aquavitas_E24 aquavitas_E24 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639554 aquavitas_E25 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592920 aquavitas_E25 aquavitas_E25 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639555 aquavitas_E26 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592921 aquavitas_E26 aquavitas_E26 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639556 aquavitas_E27 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592922 aquavitas_E27 aquavitas_E27 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639557 aquavitas_E28 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592923 aquavitas_E28 aquavitas_E28 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639558 aquavitas_E29 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592924 aquavitas_E29 aquavitas_E29 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639559 aquavitas_E30 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592925 aquavitas_E30 aquavitas_E30 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639560 aquavitas_E4 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592926 aquavitas_E4 aquavitas_E4 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639561 aquavitas_E31 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592927 aquavitas_E31 aquavitas_E31 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639562 aquavitas_E33 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592928 aquavitas_E33 aquavitas_E33 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639563 aquavitas_E34 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592929 aquavitas_E34 aquavitas_E34 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639564 aquavitas_E35 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592930 aquavitas_E35 aquavitas_E35 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639565 aquavitas_E36 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592931 aquavitas_E36 aquavitas_E36 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639566 aquavitas_E37 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592932 aquavitas_E37 aquavitas_E37 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639567 aquavitas_E38 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592933 aquavitas_E38 aquavitas_E38 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639568 aquavitas_E39 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592934 aquavitas_E39 aquavitas_E39 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639569 aquavitas_E40 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592935 aquavitas_E40 aquavitas_E40 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639570 aquavitas_E41 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592936 aquavitas_E41 aquavitas_E41 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639571 aquavitas_E5 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592937 aquavitas_E5 aquavitas_E5 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639572 aquavitas_E42 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592938 aquavitas_E42 aquavitas_E42 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639573 aquavitas_E43 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592939 aquavitas_E43 aquavitas_E43 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639574 aquavitas_E44 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592940 aquavitas_E44 aquavitas_E44 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639575 aquavitas_E51 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592941 aquavitas_E51 aquavitas_E51 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639576 aquavitas_E52 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592942 aquavitas_E52 aquavitas_E52 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639577 aquavitas_E53 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592943 aquavitas_E53 aquavitas_E53 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639578 aquavitas_E54 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592944 aquavitas_E54 aquavitas_E54 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639579 aquavitas_E55 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592945 aquavitas_E55 aquavitas_E55 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639580 aquavitas_E56 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592946 aquavitas_E56 aquavitas_E56 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639581 aquavitas_E57 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592947 aquavitas_E57 aquavitas_E57 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639582 aquavitas_E6 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592948 aquavitas_E6 aquavitas_E6 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639583 aquavitas_E58 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592949 aquavitas_E58 aquavitas_E58 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639584 aquavitas_E59 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592950 aquavitas_E59 aquavitas_E59 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639585 aquavitas_E60 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592951 aquavitas_E60 aquavitas_E60 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639586 aquavitas_E61 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592952 aquavitas_E61 aquavitas_E61 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639587 aquavitas_E62 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592953 aquavitas_E62 aquavitas_E62 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639588 aquavitas_E63 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592954 aquavitas_E63 aquavitas_E63 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639589 aquavitas_E64 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592955 aquavitas_E64 aquavitas_E64 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639590 aquavitas_E65 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592956 aquavitas_E65 aquavitas_E65 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639591 aquavitas_E66 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592957 aquavitas_E66 aquavitas_E66 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639592 aquavitas_E67 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592958 aquavitas_E67 aquavitas_E67 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639593 aquavitas_E7 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592959 aquavitas_E7 aquavitas_E7 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639594 aquavitas_E68 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592960 aquavitas_E68 aquavitas_E68 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639595 aquavitas_E75 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592961 aquavitas_E75 aquavitas_E75 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639596 aquavitas_E76 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592962 aquavitas_E76 aquavitas_E76 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639597 aquavitas_E77 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592963 aquavitas_E77 aquavitas_E77 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639598 aquavitas_E78 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592964 aquavitas_E78 aquavitas_E78 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639599 aquavitas_E79 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592965 aquavitas_E79 aquavitas_E79 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639600 aquavitas_E80 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592966 aquavitas_E80 aquavitas_E80 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639601 aquavitas_E81 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592967 aquavitas_E81 aquavitas_E81 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639602 aquavitas_E82 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592968 aquavitas_E82 aquavitas_E82 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639603 aquavitas_E84 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592969 aquavitas_E84 aquavitas_E84 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639604 aquavitas_E8 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592970 aquavitas_E8 aquavitas_E8 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639605 aquavitas_E85 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592971 aquavitas_E85 aquavitas_E85 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639606 aquavitas_E86 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592972 aquavitas_E86 aquavitas_E86 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639607 aquavitas_E87 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592973 aquavitas_E87 aquavitas_E87 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639608 aquavitas_E101 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592974 aquavitas_E101 aquavitas_E101 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639609 aquavitas_E102 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592975 aquavitas_E102 aquavitas_E102 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639610 aquavitas_E103 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592976 aquavitas_E103 aquavitas_E103 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639611 aquavitas_E104 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592977 aquavitas_E104 aquavitas_E104 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639612 aquavitas_E105 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592978 aquavitas_E105 aquavitas_E105 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639613 aquavitas_E106 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592980 aquavitas_E106 aquavitas_E106 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639614 aquavitas_E107 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592979 aquavitas_E107 aquavitas_E107 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639615 aquavitas_E9 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592981 aquavitas_E9 aquavitas_E9 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639616 aquavitas_E108 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592982 aquavitas_E108 aquavitas_E108 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639617 aquavitas_E109 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592983 aquavitas_E109 aquavitas_E109 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639618 aquavitas_E110 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592984 aquavitas_E110 aquavitas_E110 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639619 aquavitas_E111 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592985 aquavitas_E111 aquavitas_E111 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639620 aquavitas_E112 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592986 aquavitas_E112 aquavitas_E112 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639621 aquavitas_E113 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592987 aquavitas_E113 aquavitas_E113 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639622 aquavitas_E114 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592988 aquavitas_E114 aquavitas_E114 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639623 aquavitas_E115 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592989 aquavitas_E115 aquavitas_E115 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639624 aquavitas_E116 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592990 aquavitas_E116 aquavitas_E116 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639625 aquavitas_E117 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592991 aquavitas_E117 aquavitas_E117 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639626 aquavitas_E10 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592992 aquavitas_E10 aquavitas_E10 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639627 aquavitas_E118 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592993 aquavitas_E118 aquavitas_E118 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639628 aquavitas_E119 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592994 aquavitas_E119 aquavitas_E119 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639629 aquavitas_E120 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592995 aquavitas_E120 aquavitas_E120 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639630 aquavitas_E121 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592996 aquavitas_E121 aquavitas_E121 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639631 aquavitas_E122 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592997 aquavitas_E122 aquavitas_E122 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639632 aquavitas_E123 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592998 aquavitas_E123 aquavitas_E123 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639633 aquavitas_E124 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10592999 aquavitas_E124 aquavitas_E124 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639634 aquavitas_E125 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10593000 aquavitas_E125 aquavitas_E125 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639635 aquavitas_E126 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10593001 aquavitas_E126 aquavitas_E126 AMPLICON VIRAL RNA PCR NextSeq 500 SRX12639636 aquavitas_E127 SRP341648 PRJNA771693 RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons will be generated by multiplex PCR using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the artic V4 primers (https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V4) then prepared for sequencing using the PlexWell 384 Library Prep Kit (SeqWell) following manufacturers instructions, or generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries will be sequenced on a NextSeq500/550 (Illumina) at 2x150 targeting a total read count >= 430k paired end reads per sample. SRS10593002 aquavitas_E127 aquavitas_E127 AMPLICON VIRAL RNA PCR NextSeq 500