SRX12665093 L1-P1-10LR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-10LR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665094 L1-P1-10RR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-10RR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665095 L1-P1-14RF-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-14RF-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665096 L1-P2-12RR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-12RR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665097 L1-P2-13LF-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-13LF-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665098 L1-P2-13RR-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-13RR-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665099 L1-P2-17LR-W8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-17LR-W8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665100 L1-P2-18LF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-18LF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665101 L1-P2-18RF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-18RF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665102 L1-P2-1RR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-1RR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665103 L1-P2-20LF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-20LF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665104 L1-P2-20RF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-20RF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665105 L1-P2-23LR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-23LR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665106 L1-P1-14RR-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-14RR-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665107 L1-P2-23LR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-23LR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665108 L1-P2-23RF-WK7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-23RF-WK7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665109 L2-P7-38LF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-38LF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665110 L2-P7-38LF-W8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-38LF-W8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665111 L2-P7-39LF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-39LF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665112 L2-P7-39LR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-39LR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665113 L2-P7-39RR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-39RR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665114 L2-P7-3LF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-3LF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665115 L2-P7-3LR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-3LR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665116 L2-P7-3LR-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-3LR-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665117 L2-P7-3LR-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-3LR-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665118 L1-P1-42LR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-42LR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665119 L2-P7-40LR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-40LR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665120 L2-P7-40LR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-40LR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665121 L2-P7-40RF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-40RF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665122 L2-P7-40RR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-40RR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665123 L2-P7-42RF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-42RF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665124 L2-P7-4LR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-4LR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665125 L1-P4-BLANK-B4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-BLANK-B4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665126 L1-P4-BLANK-B4-18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-BLANK-B4-18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665127 L1-P4-BLANK-B41 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-BLANK-B41 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665128 L1-P4-BLANK-B7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-BLANK-B7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665129 L1-P4-BLANK-VE-11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-BLANK-VE-11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665130 L1-P4-BLANK-VE-16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-BLANK-VE-16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665131 L1-P4-BLANK-VE-5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-BLANK-VE-5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665132 L1-P4-BLANK-VE-9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-BLANK-VE-9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665133 L1-P1-28LF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-28LF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665134 L1-P4-MC-4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-MC-4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665135 L1-P4-PC1-N1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-PC1-N1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665136 L1-P4-PC2-N2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-PC2-N2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665137 L1-P4-PC3-N3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-PC3-N3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665138 L2-P5-10RF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-10RF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665139 L2-P5-11LR-W14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-11LR-W14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665140 L2-P5-12LF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-12LF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665141 L1-P3-23RF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-23RF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665142 L1-P3-23RR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-23RR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665143 L1-P1-19RR-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-19RR-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665144 L1-P3-24RR-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-24RR-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665145 L1-P3-24RR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-24RR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665146 L1-P3-25LF-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-25LF-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665147 L1-P3-25LF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-25LF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665148 L1-P3-25LF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-25LF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665149 L1-P3-25RR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-25RR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665150 L1-P3-26LF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-26LF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665151 L1-P3-26RF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-26RF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665152 L1-P3-27LF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-27LF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665153 L1-P3-27LR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-27LR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665154 L1-P1-1RF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-1RF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665155 L1-P3-27RR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-27RR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665156 L1-P3-30LF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-30LF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665157 L2-P5-12LR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-12LR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665158 L2-P5-12LR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-12LR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665159 L2-P5-12RF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-12RF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665160 L1-P1-28RF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-28RF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665161 L2-P5-15RR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-15RR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665162 L2-P5-15RR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-15RR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665163 L2-P5-16LF-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-16LF-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665164 L2-P5-17LF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-17LF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665165 L2-P5-19LF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-19LF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665166 L2-P5-19RF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-19RF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665167 L2-P5-1LR-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-1LR-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665168 L2-P5-1RR-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-1RR-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665169 L2-P5-25RF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-25RF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665170 L2-P5-25RF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-25RF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665171 L1-P1-11LR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-11LR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665172 L1-P1-2LF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-2LF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665173 L2-P7-50RF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-50RF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665174 L2-P7-5LF-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-5LF-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665175 L2-P7-5RR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-5RR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665176 L2-P7-6LF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-6LF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665177 L1-P1-42RR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-42RR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665178 L2-P7-7LF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-7LF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665179 L2-P7-7LR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-7LR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665180 L2-P7-8LR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-8LR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665181 L2-P7-8RF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-8RF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665182 L2-P7-9LF-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-9LF-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665183 L2-P7-9LF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-9LF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665184 L2-P7-9LR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-9LR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665185 L2-P7-9LR-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-9LR-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665186 L2-P7-9LR-W8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-9LR-W8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665187 L2-P7-BLANK-B10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-BLANK-B10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665188 L1-P1-47LF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-47LF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665189 L1-P2-23RR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-23RR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665190 L1-P2-24LF-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-24LF-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665191 L1-P2-24LR-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-24LR-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665192 L1-P2-26LF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-26LF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665193 L1-P2-26RR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-26RR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665194 L1-P2-27LR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-27LR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665195 L1-P2-28LF-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-28LF-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665196 L1-P2-2LF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-2LF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665197 L1-P1-15LR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-15LR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665198 L1-P2-30LR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-30LR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665199 L1-P2-30RF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-30RF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665200 L1-P2-30RR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-30RR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665201 L1-P2-31LR-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-31LR-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665202 L1-P2-31RR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-31RR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665203 L1-P2-31RR-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-31RR-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665204 L1-P2-32RR-W14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-32RR-W14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665205 L1-P3-50RR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-50RR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665206 L1-P3-5LF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-5LF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665207 L1-P3-5LR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-5LR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665208 L1-P1-22RF-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-22RF-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665209 L1-P3-5RF-W8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-5RF-W8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665210 L1-P3-5RR-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-5RR-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665211 L1-P3-6LR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-6LR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665212 L1-P3-6RR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-6RR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665213 L1-P3-7LF-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-7LF-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665214 L1-P3-7LR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-7LR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665215 L1-P3-7LR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-7LR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665216 L1-P3-7RF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-7RF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665217 L1-P3-7RR-W8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-7RR-W8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665218 L1-P3-7RR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-7RR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665219 L1-P1-23LR-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-23LR-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665220 L1-P3-9RF-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-9RF-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665221 L1-P1-37LR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-37LR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665222 L2-P6-9LF-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-9LF-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665223 L2-P6-9RR-W14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-9RR-W14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665224 L2-P6-9RR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-9RR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665225 L2-P6-BLANK-B11-18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-BLANK-B11-18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665226 L2-P6-BLANK-B2-12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-BLANK-B2-12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665227 L2-P6-BLANK-B29 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-BLANK-B29 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665228 L2-P6-BLANK-B34 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-BLANK-B34 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665229 L2-P6-BLANK-B37 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-BLANK-B37 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665230 L2-P6-BLANK-B42 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-BLANK-B42 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665231 L2-P6-BLANK-B45 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-BLANK-B45 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665232 L1-P1-37RR-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-37RR-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665233 L2-P6-BLANK-B9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-BLANK-B9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665234 L2-P6-BLANK-VE-8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-BLANK-VE-8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665235 L2-P6-MC-6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-MC-6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665236 L2-P6-PC1-N1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-PC1-N1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665237 L1-P2-7RR-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-7RR-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665238 L1-P2-7RR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-7RR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665239 L1-P2-8RR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-8RR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665240 L1-P2-8RR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-8RR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665241 L1-P2-9LR-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-9LR-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665242 L1-P2-9LRF-W14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-9LRF-W14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665243 L1-P2-BLANK-B10-18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-BLANK-B10-18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665244 L1-P2-BLANK-B2-18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-BLANK-B2-18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665245 L1-P2-BLANK-B2-5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-BLANK-B2-5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665246 L1-P2-BLANK-B21 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-BLANK-B21 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665247 L1-P1-17LF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-17LF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665248 L1-P2-BLANK-B22 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-BLANK-B22 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665249 L1-P2-BLANK-B26 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-BLANK-B26 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665250 L1-P2-BLANK-B2a-18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-BLANK-B2a-18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665251 L1-P2-BLANK-B30 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-BLANK-B30 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665252 L1-P2-BLANK-B39 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-BLANK-B39 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665253 L2-P7-BLANK-B11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-BLANK-B11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665254 L2-P7-BLANK-B12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-BLANK-B12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665255 L2-P7-BLANK-B3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-BLANK-B3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665256 L2-P7-BLANK-B44 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-BLANK-B44 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665257 L2-P7-BLANK-B8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-BLANK-B8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665258 L2-P7-BLANK-B9-18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-BLANK-B9-18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665259 L2-P7-BLANK-VE-2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-BLANK-VE-2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665260 L2-P7-BLANK-VE-4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-BLANK-VE-4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665261 L2-P7-MC-7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-MC-7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665262 L2-P7-PC1-N1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-PC1-N1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665263 L1-P1-47LR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-47LR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665264 L2-P7-PC2-N2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-PC2-N2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665265 L2-P7-PC3-N3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-PC3-N3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665266 L2-P8-10LR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-10LR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665267 L2-P8-10RF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-10RF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665268 L2-P8-10RR-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-10RR-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665269 L1-P3-17RR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-17RR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665270 L1-P3-17RR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-17RR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665271 L1-P3-19LF-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-19LF-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665272 L1-P3-19LR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-19LR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665273 L1-P3-19RF-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-19RF-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665274 L1-P3-1LF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-1LF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665275 L1-P3-1LF-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-1LF-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665276 L1-P1-19RF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-19RF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665277 L1-P3-1RR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-1RR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665278 L1-P3-20RR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-20RR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665279 L1-P3-22RR-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-22RR-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665280 L1-P3-23LF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-23LF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665281 L1-P3-23LF-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-23LF-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665282 L1-P3-23LF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-23LF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665283 L1-P3-23RF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-23RF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665284 L1-P3-23RF-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-23RF-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665285 L1-P3-33LR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-33LR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665286 L1-P3-35LF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-35LF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665287 L1-P3-35LF-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-35LF-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665288 L1-P3-35RF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-35RF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665289 L1-P3-35RF-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-35RF-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665290 L1-P3-35RR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-35RR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665291 L1-P3-35RR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-35RR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665292 L1-P3-36RF-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-36RF-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665293 L1-P1-20LF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-20LF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665294 L1-P3-37LR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-37LR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665295 L1-P3-3LF-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-3LF-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665296 L1-P3-3RR-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-3RR-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665297 L1-P3-40RF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-40RF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665298 L1-P3-40RR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-40RR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665299 L1-P3-48LR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-48LR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665300 L1-P3-4RR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-4RR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665301 L2-P5-BLANK-VE-14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-BLANK-VE-14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665302 L2-P5-BLANK-VE-6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-BLANK-VE-6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665303 L2-P5-BLANK-VE-7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-BLANK-VE-7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665304 L2-P5-MC-5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-MC-5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665305 L2-P5-PC1-N1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-PC1-N1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665306 L2-P5-PC2-N2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-PC2-N2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665307 L2-P5-PC3-N3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-PC3-N3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665308 L1-P1-31RR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-31RR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665309 L2-P6-11LF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-11LF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665310 L2-P6-11LR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-11LR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665311 L2-P6-11LR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-11LR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665312 L2-P6-11RF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-11RF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665313 L2-P6-11RR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-11RR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665314 L2-P6-12LF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-12LF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665315 L2-P6-12LF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-12LF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665316 L2-P6-12LR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-12LR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665317 L2-P6-22LF-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-22LF-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665318 L2-P6-22LF-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-22LF-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665319 L2-P6-22LR-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-22LR-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665320 L2-P6-22LR-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-22LR-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665321 L2-P6-22RR-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-22RR-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665322 L2-P6-23LR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-23LR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665323 L2-P6-23RF-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-23RF-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665324 L2-P6-24LR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-24LR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665325 L2-P6-25LR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-25LR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665326 L1-P1-34RF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-34RF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665327 L2-P6-26LF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-26LF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665328 L2-P6-26LR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-26LR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665329 L2-P6-26RF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-26RF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665330 L2-P6-27LF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-27LF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665331 L2-P6-2LF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-2LF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665332 L2-P6-2LR-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-2LR-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665333 L1-P2-40LR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-40LR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665334 L1-P2-40RF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-40RF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665335 L1-P2-40RR-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-40RR-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665336 L1-P2-40RR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-40RR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665337 L1-P1-16RF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-16RF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665338 L1-P2-4LR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-4LR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665339 L1-P2-4RR-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-4RR-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665340 L1-P2-50LR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-50LR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665341 L1-P2-5LF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-5LF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665342 L1-P2-5LF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-5LF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665343 L1-P2-5LF-W8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-5LF-W8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665344 L1-P2-5LR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-5LR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665345 L1-P2-5RF-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-5RF-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665346 L1-P2-5RF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-5RF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665347 L1-P2-7LF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-7LF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665348 L1-P1-16RR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-16RR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665349 L2-P5-25RR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-25RR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665350 L2-P5-26LF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-26LF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665351 L2-P5-2LR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-2LR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665352 L2-P5-2RF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-2RF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665353 L2-P5-30LF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-30LF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665354 L2-P5-30LR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-30LR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665355 L2-P5-30RR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-30RR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665356 L2-P5-31RF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-31RF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665357 L2-P5-31RF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-31RF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665358 L2-P5-33LF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-33LF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665359 L1-P1-2LR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-2LR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665360 L2-P5-33RF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-33RF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665361 L2-P5-33RR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-33RR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665362 L2-P5-34LR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-34LR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665363 L2-P5-34LR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-34LR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665364 L2-P5-34RR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-34RR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665365 L2-P7-16RR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-16RR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665366 L1-P1-3LF-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-3LF-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665367 L2-P7-17LR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-17LR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665368 L2-P7-17RF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-17RF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665369 L2-P7-18LF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-18LF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665370 L2-P7-18LR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-18LR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665371 L2-P7-18LR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-18LR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665372 L2-P7-18RF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-18RF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665373 L2-P7-19LF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-19LF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665374 L2-P7-19LR-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-19LR-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665375 L2-P7-19RR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-19RR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665376 L2-P7-19RR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-19RR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665377 L1-P1-12LF-W26 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-12LF-W26 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665378 L1-P1-3RF-W14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-3RF-W14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665379 L2-P7-1LR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-1LR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665380 L2-P7-20LF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-20LF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665381 L2-P8-MC-8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-MC-8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665382 L2-P8-PC1-N1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-PC1-N1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665383 L2-P8-PC2-N2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-PC2-N2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665384 L2-P8-PC3-N3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-PC3-N3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665385 L1-P1-7RF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-7RF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665386 L1-P1-8LF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-8LF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665387 L1-P1-BLANK-B1-18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-BLANK-B1-18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665388 L1-P1-BLANK-B2-11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-BLANK-B2-11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665389 L1-P1-BLANK-B2-17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-BLANK-B2-17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665390 L1-P1-BLANK-B2-19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-BLANK-B2-19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665391 L1-P1-12RF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-12RF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665392 L1-P1-BLANK-B2-4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-BLANK-B2-4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665393 L1-P1-BLANK-B2-9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-BLANK-B2-9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665394 L1-P1-BLANK-B3-18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-BLANK-B3-18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665395 L1-P1-BLANK-B5-18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-BLANK-B5-18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665396 L1-P1-BLANK-B6-18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-BLANK-B6-18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665397 L1-P1-25LR-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-25LR-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665398 L1-P4-12RR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-12RR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665399 L1-P4-13RF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-13RF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665400 L1-P4-14LR-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-14LR-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665401 L1-P4-17LF-W8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-17LF-W8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665402 L1-P4-17LR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-17LR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665403 L1-P4-17LR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-17LR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665404 L1-P4-17RF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-17RF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665405 L1-P4-1LF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-1LF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665406 L1-P4-1LR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-1LR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665407 L1-P4-20LF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-20LF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665408 L1-P1-25RR-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-25RR-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665409 L1-P4-20LR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-20LR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665410 L1-P4-20RR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-20RR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665411 L1-P4-22RF-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-22RF-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665412 L1-P4-23LF-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-23LF-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665413 L2-P6-30LF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-30LF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665414 L2-P6-30RF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-30RF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665415 L2-P6-31LR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-31LR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665416 L2-P6-31RR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-31RR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665417 L1-P1-34RR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-34RR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665418 L2-P6-32LF-W14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-32LF-W14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665419 L2-P6-32LR-W14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-32LR-W14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665420 L2-P6-32RF-W14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-32RF-W14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665421 L2-P6-33RF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-33RF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665422 L2-P6-34RF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-34RF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665423 L2-P6-34RR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-34RR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665424 L2-P6-36RR-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-36RR-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665425 L2-P6-37LF-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-37LF-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665426 L2-P6-37LR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-37LR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665427 L2-P6-37LR-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-37LR-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665428 L1-P1-35LR-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-35LR-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665429 L2-P6-PC2-N2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-PC2-N2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665430 L2-P6-PC3-N3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-PC3-N3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665431 L2-P7-10LF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-10LF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665432 L2-P7-10LR-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-10LR-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665433 L2-P7-11RF-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-11RF-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665434 L2-P7-11RR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-11RR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665435 L1-P1-39LF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-39LF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665436 L2-P7-12LR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-12LR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665437 L1-P2-38RF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-38RF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665438 L1-P2-39LF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-39LF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665439 L1-P2-39LF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-39LF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665440 L1-P2-39RF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-39RF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665441 L1-P2-39RR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-39RR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665442 L1-P2-3LF-W14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-3LF-W14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665443 L1-P2-3LF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-3LF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665444 L1-P2-3RF-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-3RF-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665445 L1-P2-3RR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-3RR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665446 L1-P1-15RR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-15RR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665447 L1-P2-3RR-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-3RR-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665448 L1-P2-3RR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-3RR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665449 L1-P2-40LF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-40LF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665450 L1-P2-40LF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-40LF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665451 L1-P2-40LF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-40LF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665452 L1-P2-40LR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-40LR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665453 L2-P5-40RF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-40RF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665454 L2-P5-40RR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-40RR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665455 L2-P5-48RF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-48RF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665456 L2-P5-4LR-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-4LR-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665457 L2-P5-5LR-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-5LR-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665458 L2-P5-5RF-2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-5RF-2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665459 L1-P1-2RR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-2RR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665460 L2-P5-5RF-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-5RF-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665461 L2-P5-7LR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-7LR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665462 L2-P5-7LR-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-7LR-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665463 L2-P5-7RR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-7RR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665464 L2-P5-8LF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-8LF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665465 L2-P5-8RF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-8RF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665466 L2-P5-8RF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-8RF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665467 L2-P5-9LF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-9LF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665468 L2-P5-9LR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-9LR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665469 L2-P8-4RF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-4RF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665470 L2-P8-4RF-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-4RF-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665471 L1-P1-7LR-W8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-7LR-W8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665472 L2-P8-5RF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-5RF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665473 L2-P8-6LR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-6LR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665474 L2-P8-6RF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-6RF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665475 L2-P8-7LR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-7LR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665476 L2-P8-7RR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-7RR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665477 L2-P8-8LR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-8LR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665478 L2-P8-9RF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-9RF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665479 L2-P8-9RF-W8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-9RF-W8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665480 L2-P8-9RR-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-9RR-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665481 L2-P8-BLANK-B15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-BLANK-B15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665482 L1-P1-7RF-W8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-7RF-W8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665483 L2-P8-BLANK-B2-6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-BLANK-B2-6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665484 L2-P8-BLANK-VE-10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-BLANK-VE-10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665485 L2-P7-12RF-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-12RF-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665486 L2-P7-12RR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-12RR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665487 L2-P7-12RR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-12RR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665488 L2-P7-13LF-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-13LF-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665489 L2-P7-13LF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-13LF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665490 L2-P7-15LR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-15LR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665491 L2-P7-16LF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-16LF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665492 L2-P7-16LR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-16LR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665493 L1-P2-33LF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-33LF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665494 L1-P2-33RF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-33RF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665495 L1-P2-34LF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-34LF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665496 L1-P1-15RF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-15RF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665497 L1-P2-35LF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-35LF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665498 L1-P2-35LF-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-35LF-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665499 L1-P2-35RR-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-35RR-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665500 L1-P2-36LF-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-36LF-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665501 L1-P2-36LR-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-36LR-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665502 L1-P2-36LR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-36LR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665503 L1-P2-36RR-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-36RR-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665504 L1-P2-37LF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-37LF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665505 L1-P2-38LR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-38LR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665506 L1-P2-38LR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-38LR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665507 L1-P1-15RF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-15RF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665508 L1-P2-38RF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-38RF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665509 L1-P4-34LR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-34LR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665510 L1-P1-26RR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-26RR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665511 L1-P4-35RF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-35RF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665512 L1-P4-35RF-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-35RF-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665513 L1-P4-36LR-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-36LR-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665514 L1-P4-36RF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-36RF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665515 L1-P4-37RR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-37RR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665516 L1-P4-38LR-W8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-38LR-W8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665517 L1-P4-38RR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-38RR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665518 L1-P4-39LF-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-39LF-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665519 L1-P4-39LR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-39LR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665520 L1-P4-39LR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-39LR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665521 L1-P1-27LF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-27LF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665522 L1-P4-3LF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-3LF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665523 L1-P4-3LF-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-3LF-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665524 L1-P4-3LR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-3LR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665525 L2-P8-11RF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-11RF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665526 L2-P8-12LF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-12LF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665527 L2-P8-12RF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-12RF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665528 L2-P8-13LR-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-13LR-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665529 L2-P8-13LR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-13LR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665530 L1-P1-47RR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-47RR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665531 L2-P8-15LF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-15LF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665532 L2-P8-16RF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-16RF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665533 L2-P8-18RR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-18RR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665534 L2-P8-1RF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-1RF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665535 L2-P8-20LR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-20LR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665536 L2-P8-23LF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-23LF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665537 L2-P8-23RF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-23RF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665538 L2-P8-23RR-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-23RR-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665539 L2-P8-24LF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-24LF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665540 L2-P8-25LF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-25LF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665541 L2-P5-9RF-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-9RF-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665542 L1-P1-31LR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-31LR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665543 L2-P5-9RF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-9RF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665544 L2-P5-9RR-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-9RR-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665545 L2-P5-BLANK-B12-18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-BLANK-B12-18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665546 L2-P5-BLANK-B13-18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-BLANK-B13-18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665547 L2-P5-BLANK-B2-10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-BLANK-B2-10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665548 L2-P5-BLANK-B2-13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-BLANK-B2-13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665549 L2-P5-BLANK-B2-14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-BLANK-B2-14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665550 L2-P5-BLANK-B2-20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-BLANK-B2-20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665551 L2-P5-BLANK-B2-8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-BLANK-B2-8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665552 L2-P5-BLANK-B24 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-BLANK-B24 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665553 L1-P1-31RF-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-31RF-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665554 L2-P5-BLANK-B27 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-BLANK-B27 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665555 L2-P5-BLANK-B33 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-BLANK-B33 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665556 L2-P5-BLANK-B43 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-BLANK-B43 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665557 L2-P6-37RF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-37RF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665558 L2-P6-37RR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-37RR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665559 L2-P6-37RR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-37RR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665560 L2-P6-38LF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-38LF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665561 L2-P6-38LF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-38LF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665562 L2-P6-38RR-W8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-38RR-W8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665563 L2-P6-39RF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-39RF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665564 L2-P6-39RR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-39RR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665565 L2-P6-3RF-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-3RF-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665566 L2-P6-3RR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-3RR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665567 L1-P1-35RF-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-35RF-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665568 L2-P6-40RR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-40RR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665569 L2-P6-4LF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-4LF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665570 L2-P6-50LF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-50LF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665571 L2-P6-5LF-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-5LF-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665572 L2-P6-5LR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-5LR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665573 L1-P3-BLANK-B1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-BLANK-B1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665574 L1-P3-BLANK-B13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-BLANK-B13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665575 L1-P3-BLANK-B16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-BLANK-B16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665576 L1-P3-BLANK-B18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-BLANK-B18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665577 L1-P3-BLANK-B19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-BLANK-B19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665578 L1-P3-BLANK-B2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-BLANK-B2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665579 L1-P3-BLANK-B2-1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-BLANK-B2-1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665580 L1-P3-BLANK-B2-2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-BLANK-B2-2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665581 L1-P3-BLANK-B2-3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-BLANK-B2-3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665582 L1-P1-23LR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-23LR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665583 L1-P3-BLANK-B20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-BLANK-B20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665584 L1-P3-BLANK-B5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-BLANK-B5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665585 L1-P3-BLANK-B6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-BLANK-B6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665586 L1-P3-BLANK-VE-19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-BLANK-VE-19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665587 L1-P3-MC-3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-MC-3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665588 L1-P3-PC1-N1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-PC1-N1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665589 L2-P7-20LR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-20LR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665590 L2-P7-20RF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-20RF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665591 L2-P7-20RF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-20RF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665592 L2-P7-20RF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-20RF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665593 L2-P7-20RR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-20RR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665594 L2-P7-23LR-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-23LR-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665595 L2-P7-23LR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-23LR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665596 L2-P7-23RR-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-23RR-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665597 L1-P1-3RF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-3RF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665598 L2-P7-23RR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-23RR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665599 L2-P7-23RR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-23RR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665600 L2-P7-24RF-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-24RF-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665601 L2-P7-24RF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-24RF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665602 L2-P7-25LR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-25LR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665603 L2-P7-26LR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-26LR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665604 L2-P7-26LR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-26LR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665605 L1-P4-8LR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-8LR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665606 L1-P4-9LF-W8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-9LF-W8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665607 L1-P1-27RF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-27RF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665608 L1-P4-9LR-W14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-9LR-W14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665609 L1-P4-9RR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-9RR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665610 L1-P4-BLANK-B14-18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-BLANK-B14-18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665611 L1-P4-BLANK-B17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-BLANK-B17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665612 L1-P4-BLANK-B2-15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-BLANK-B2-15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665613 L1-P4-BLANK-B2-16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-BLANK-B2-16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665614 L1-P4-BLANK-B2-7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-BLANK-B2-7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665615 L1-P4-BLANK-B25 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-BLANK-B25 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665616 L1-P4-BLANK-B28 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-BLANK-B28 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665617 L1-P4-BLANK-B31 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-BLANK-B31 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665618 L1-P1-27RR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-27RR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665619 L1-P4-BLANK-B36 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-BLANK-B36 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665620 L1-P4-BLANK-B38 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-BLANK-B38 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665621 L1-P1-5RR-WK8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-5RR-WK8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665622 L2-P8-26RF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-26RF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665623 L2-P8-28LR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-28LR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665624 L2-P8-28LR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-28LR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665625 L2-P8-28RF-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-28RF-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665626 L2-P8-28RR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-28RR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665627 L2-P8-2LR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-2LR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665628 L2-P8-31LF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-31LF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665629 L2-P8-31LR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-31LR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665630 L2-P8-31RF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-31RF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665631 L2-P8-33RR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-33RR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665632 L1-P1-12LF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-12LF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665633 L1-P1-6RR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-6RR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665634 L2-P8-34LF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-34LF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665635 L2-P8-34RF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-34RF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665636 L2-P8-35LR-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-35LR-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665637 L1-P4-3RR-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-3RR-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665638 L1-P4-40LF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-40LF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665639 L1-P4-40LF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-40LF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665640 L1-P4-40RR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-40RR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665641 L1-P4-47RF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-47RF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665642 L1-P4-48LF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-48LF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665643 L1-P4-4LF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-4LF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665644 L1-P1-27LR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-27LR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665645 L1-P4-4LF-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-4LF-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665646 L1-P4-4RF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-4RF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665647 L1-P4-4RR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-4RR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665648 L1-P4-5RR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-5RR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665649 L1-P4-7LF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-7LF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665650 L1-P4-7LR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-7LR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665651 L1-P4-7RF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-7RF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665652 L1-P4-7RF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-7RF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665653 L2-P6-5LR-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-5LR-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665654 L2-P6-5LR-W8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-5LR-W8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665655 L2-P6-5RR-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-5RR-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665656 L2-P6-5RR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-5RR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665657 L2-P6-6LF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-6LF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665658 L1-P1-36RF-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-36RF-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665659 L2-P6-6RF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-6RF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665660 L2-P6-7LF-W8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-7LF-W8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665661 L2-P6-7LF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-7LF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665662 L2-P6-7LR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-7LR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665663 L2-P6-7RF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-7RF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665664 L2-P6-7RF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-7RF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665665 L2-P6-7RR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-7RR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665666 L2-P6-7RR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-7RR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665667 L2-P6-8LF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-8LF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665668 L2-P6-8RR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-8RR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665669 L1-P3-PC2-N2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-PC2-N2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665670 L1-P3-PC3-N3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-PC3-N3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665671 L1-P4-10LF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-10LF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665672 L1-P4-10LR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-10LR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665673 L1-P1-23RR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-23RR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665674 L1-P4-10RR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-10RR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665675 L1-P4-11LF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-11LF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665676 L1-P4-11LF-W14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-11LF-W14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665677 L1-P4-11LR-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-11LR-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665678 L1-P4-11RF-W14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-11RF-W14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665679 L1-P4-11RF-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-11RF-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665680 L1-P4-11RR-W14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-11RR-W14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665681 L1-P4-12LR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-12LR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665682 L1-P4-12LR-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-12LR-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665683 L1-P4-12RF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-12RF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665684 L1-P1-11LR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-11LR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665685 L1-P1-BLANK-B7-18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-BLANK-B7-18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665686 L1-P1-BLANK-B8-18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-BLANK-B8-18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665687 L1-P1-BLANK-VE-1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-BLANK-VE-1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665688 L1-P1-BLANK-VE-12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-BLANK-VE-12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665689 L1-P1-BLANK-VE-13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-BLANK-VE-13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665690 L1-P1-12RR-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-12RR-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665691 L1-P1-BLANK-VE-15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-BLANK-VE-15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665692 L1-P1-BLANK-VE-20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-BLANK-VE-20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665693 L1-P1-MC-1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-MC-1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665694 L1-P1-PC1-N1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-PC1-N1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665695 L1-P1-PC2-N2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-PC2-N2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665696 L1-P1-PC3-N3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-PC3-N3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665697 L1-P2-10RF-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-10RF-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665698 L1-P2-11RF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-11RF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665699 L1-P2-11RF-W11a SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-11RF-W11a AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665700 L1-P2-11RR-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-11RR-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665701 L2-P6-15LF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-15LF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665702 L2-P6-15RF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-15RF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665703 L1-P1-33LF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-33LF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665704 L2-P6-16LF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-16LF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665705 L2-P6-16RF-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-16RF-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665706 L2-P6-17LF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-17LF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665707 L2-P6-17LF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-17LF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665708 L2-P6-17RF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-17RF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665709 L2-P6-17RR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-17RR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665710 L2-P6-17RR-W8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-17RR-W8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665711 L2-P6-19LR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-19LR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665712 L2-P6-1RF-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-1RF-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665713 L2-P6-20LR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-20LR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665714 L1-P1-11RR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-11RR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665715 L1-P1-33LR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-33LR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665716 L2-P6-20RR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P6-20RR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665717 L1-P2-BLANK-B40 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-BLANK-B40 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665718 L1-P2-BLANK-VE-17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-BLANK-VE-17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665719 L1-P2-BLANK-VE-3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-BLANK-VE-3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665720 L1-P2-MC-2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-MC-2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665721 L1-P2-PC1-N1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-PC1-N1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665722 L1-P1-17LR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-17LR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665723 L1-P2-PC2-N2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-PC2-N2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665724 L1-P2-PC3-N3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P2-PC3-N3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665725 L1-P3-10LF-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-10LF-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665726 L1-P3-10LF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-10LF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665727 L1-P3-10RF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-10RF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665728 L1-P3-11LF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-11LF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665729 L1-P3-11LF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-11LF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665730 L1-P3-11RR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-11RR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665731 L1-P3-12RF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-12RF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665732 L1-P3-12RR-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-12RR-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665733 L1-P1-10RR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-10RR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665734 L1-P1-17RR-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-17RR-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665735 L1-P3-13RR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-13RR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665736 L1-P3-14LF-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-14LF-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665737 L1-P3-15LF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-15LF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665738 L1-P3-15LF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-15LF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665739 L1-P3-15LR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-15LR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665740 L1-P3-15LR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-15LR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665741 L1-P3-15RF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-15RF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665742 L1-P3-15RR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-15RR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665743 L1-P3-16LR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-16LR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665744 L1-P3-16LR-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-16LR-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665745 L1-P1-18RR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-18RR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665746 L1-P3-16RR-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-16RR-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665747 L1-P3-17RF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-17RF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665748 L1-P3-17RF-W8 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P3-17RF-W8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665749 L1-P4-23LF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-23LF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665750 L1-P4-23RF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-23RF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665751 L1-P4-25RF-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-25RF-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665752 L1-P4-26RF-W11 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-26RF-W11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665753 L1-P4-26RR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-26RR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665754 L1-P4-27RF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-27RF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665755 L1-P1-26LR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-26LR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665756 L1-P4-28LF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-28LF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665757 L1-P4-28RR-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-28RR-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665758 L1-P4-2LF-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-2LF-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665759 L1-P4-30RF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-30RF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665760 L1-P4-30RR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-30RR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665761 L1-P4-31LF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-31LF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665762 L1-P4-31LF-W16 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-31LF-W16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665763 L1-P4-33LR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-33LR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665764 L1-P4-33RR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P4-33RR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665765 L2-P7-26RR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-26RR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665766 L2-P7-27RF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-27RF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665767 L2-P7-27RR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-27RR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665768 L1-P1-40LR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-40LR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665769 L2-P7-28LR-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-28LR-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665770 L2-P7-28RF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-28RF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665771 L2-P7-2RF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-2RF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665772 L2-P7-2RR-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-2RR-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665773 L2-P7-2RR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-2RR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665774 L2-P7-31LF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-31LF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665775 L2-P7-34LF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-34LF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665776 L2-P7-35LR-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-35LR-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665777 L2-P7-35RR-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-35RR-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665778 L2-P7-36LF-W14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-36LF-W14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665779 L1-P1-42LF-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-42LF-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665780 L2-P7-36RF-W14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P7-36RF-W14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665781 L2-P8-37LF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-37LF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665782 L2-P8-37RF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-37RF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665783 L2-P8-37RR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-37RR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665784 L2-P8-38RR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-38RR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665785 L2-P8-38RR-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-38RR-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665786 L2-P8-39LR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-39LR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665787 L2-P8-39RR-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-39RR-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665788 L1-P1-7LF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-7LF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665789 L2-P8-3LR-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-3LR-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665790 L2-P8-3LR-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-3LR-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665791 L2-P8-3LR-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-3LR-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665792 L2-P8-3RF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-3RF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665793 L2-P8-3RF-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-3RF-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665794 L2-P8-40LR-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-40LR-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665795 L2-P8-40RF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-40RF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665796 L2-P8-48RR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P8-48RR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665797 L2-P5-35LR-W1 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-35LR-W1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665798 L2-P5-35LR-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-35LR-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665799 L2-P5-35RR-W12 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-35RR-W12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665800 L2-P5-36LF-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-36LF-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665801 L2-P5-36LF-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-36LF-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665802 L1-P1-2RF-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-2RF-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665803 L2-P5-36RR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-36RR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665804 L2-P5-37LF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-37LF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665805 L2-P5-37LF-W3 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-37LF-W3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665806 L2-P5-37LR-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-37LR-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665807 L2-P5-37RF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-37RF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665808 L2-P5-37RF-W4 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-37RF-W4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665809 L2-P5-37RF-W7 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-37RF-W7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665810 L2-P5-38LR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-38LR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665811 L2-P5-38RF-W2 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-38RF-W2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665812 L2-P5-39LR-W18 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-39LR-W18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665813 L1-P1-2RF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-2RF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665814 L2-P5-39RF-W13 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-39RF-W13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665815 L2-P5-39RF-W15 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-39RF-W15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665816 L2-P5-39RR-W5 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-39RR-W5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665817 L2-P5-3LF-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-3LF-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665818 L2-P5-3LR-W14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-3LR-W14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665819 L2-P5-3RF-W6 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-3RF-W6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665820 L2-P5-3RF-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-3RF-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665821 L2-P5-3RR-W14 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-3RR-W14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665822 L2-P5-3RR-W20 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-3RR-W20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665823 L2-P5-40LF-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-40LF-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665824 L1-P1-2RR-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L1-P1-2RR-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665825 L2-P5-40LF-W17 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-40LF-W17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665826 L2-P5-40LR-W9 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-40LR-W9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665827 L2-P5-40RF-W10 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-40RF-W10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12665828 L2-P5-40RF-W19 SRP341852 bp0 Swab samples were collected from all four feet weekly for 20 weeks. DNA was extracted from 620 swab samples using the hydroxyapatite spin column method (Purdy, Meth Enzymol 397:271-292). An extraction control was processed with every DNA extraction run. PCR with general bacterial primers 27-YMF and 534R. MiSeq adapters were added in subsequent very short PCRS, including PCR and model community (Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis) controls in every PCR run. All samples and controls were sequenced in two 300 bp paired end sequence runs. SRS10618044 Sheep skin microbiome L2-P5-40RF-W19 AMPLICON METAGENOMIC PCR Illumina MiSeq