SRX12673727 ROSY_AMF_24 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625301 24LGB-LACT-AMFb ROSY_AMF_24 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673728 ROSY_AMF_25 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625302 25LGB-LYQU-AMFb ROSY_AMF_25 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673729 ROSY_AMF_26 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625303 26LGB-PANI-AMFb ROSY_AMF_26 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673730 ROSY_AMF_27 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625305 27LGB-SMIL-AMFb ROSY_AMF_27 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673731 ROSY_AMF_28 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625304 28LOVE-AMBR-AMFb ROSY_AMF_28 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673732 ROSY_AMF_29 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625306 29LOVE-LACT-AMFb ROSY_AMF_29 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673733 ROSY_AMF_30 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625307 30LOVE-PAQU-AMFb ROSY_AMF_30 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673734 ROSY_AMF_4 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625308 4CANEY-PANI-AMFb ROSY_AMF_4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673735 ROSY_AMF_31 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625309 31LOVE-VIOL-AMFb ROSY_AMF_31 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673736 ROSY_AMF_32 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625310 32NEON-AMBR-AMFb ROSY_AMF_32 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673737 ROSY_AMF_33 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625312 33NEON-PAQU-AMFb ROSY_AMF_33 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673738 ROSY_AMF_34 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625311 34NEON-PLMA-AMFb ROSY_AMF_34 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673739 ROSY_AMF_35 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625313 35NEON-SMIL-AMFb ROSY_AMF_35 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673740 ROSY_AMF_36 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625314 36NEON-VIOL-AMFb ROSY_AMF_36 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673741 ROSY_AMF_1 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625315 1BGB-AMPH-AMFb ROSY_AMF_1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673742 ROSY_AMF_37 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625316 37NMT-AMBR-AMFb ROSY_AMF_37 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673743 ROSY_AMF_2 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625317 2BGB-PAQU-AMFb ROSY_AMF_2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673744 ROSY_AMF_38 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625318 38NMT-LACT-AMFb ROSY_AMF_38 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673745 ROSY_AMF_39 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625319 39NMT-PAQU-AMFb ROSY_AMF_39 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673746 ROSY_AMF_40 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625320 40NMT-PLMA-AMFb ROSY_AMF_40 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673747 ROSY_AMF_5 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625321 5CANEY-PLMA-AMFb ROSY_AMF_5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673748 ROSY_AMF_41 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625322 41PL441-AMBR-AMFb ROSY_AMF_41 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673749 ROSY_AMF_43 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625323 43RGB-KALA-AMFb ROSY_AMF_43 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673750 ROSY_AMF_42 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625324 42PL441-VIOL-AMFb ROSY_AMF_42 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673751 ROSY_AMF_44 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625325 44RGB-LACT-AMFb ROSY_AMF_44 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673752 ROSY_AMF_45 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625326 45RGB-LYQU-AMFb ROSY_AMF_45 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673753 ROSY_AMF_46 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625327 46RS441-PAQU-AMFb ROSY_AMF_46 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673754 ROSY_AMF_47 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625328 47RS441-SMIL-AMFb ROSY_AMF_47 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673755 ROSY_AMF_48 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625329 48SCM-LACT-AMFb ROSY_AMF_48 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673756 ROSY_AMF_11 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625330 11DUD-SMIL-AMFb ROSY_AMF_11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673757 ROSY_AMF_49 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625331 49SCM-PANI-AMFb ROSY_AMF_49 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673758 ROSY_AMF_12 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625332 12FCL-ACER-AMFb ROSY_AMF_12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673759 ROSY_AMF_13 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625333 13FCL-PANI-AMFb ROSY_AMF_13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673760 ROSY_AMF_14 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625334 14FCL-SMIL-AMFb ROSY_AMF_14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673761 ROSY_AMF_15 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625335 15FCM-LACT-AMFb ROSY_AMF_15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673762 ROSY_AMF_16 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625336 16FCM-PANI-AMFb ROSY_AMF_16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673763 ROSY_AMF_18 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625337 18GCL-LACT-AMFb ROSY_AMF_18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673764 ROSY_AMF_17 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625338 17FCM-SMIL-AMFb ROSY_AMF_17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673765 ROSY_AMF_19 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625339 19GCL-LYQU-AMFb ROSY_AMF_19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673766 ROSY_AMF_20 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625340 20GCL-SMIL-AMFb ROSY_AMF_20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673767 ROSY_AMF_3 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625341 3CANEY-ACER-AMFb ROSY_AMF_3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673768 ROSY_AMF_21 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625342 21LCM-KALA-AMFb ROSY_AMF_21 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673769 ROSY_AMF_22 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625343 22LCM-LACT-AMFb ROSY_AMF_22 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673770 ROSY_AMF_50 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625344 50SKI-AMBR-AMFb ROSY_AMF_50 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673771 ROSY_AMF_23 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625345 23LGB-KALA-AMFb ROSY_AMF_23 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673772 ROSY_AMF_6 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625346 6CANEY-SMIL-AMFb ROSY_AMF_6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673773 ROSY_AMF_51 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625347 51SKI-PAQU-AMFb ROSY_AMF_51 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673774 ROSY_AMF_52 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625348 52TCL-LYQU-AMFb ROSY_AMF_52 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673775 ROSY_AMF_53 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625349 53TCL-PAQU-AMFb ROSY_AMF_53 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673776 ROSY_AMF_54 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625350 54TCL-PLMA-AMFb ROSY_AMF_54 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673777 ROSY_AMF_56 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625352 56WIN-ACER-AMFb ROSY_AMF_56 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673778 ROSY_AMF_55 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625351 55TCL-SMIL-AMFb ROSY_AMF_55 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673779 ROSY_AMF_57 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625353 57WIN-AMBR-AMFb ROSY_AMF_57 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673780 ROSY_AMF_58 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625354 58WIN-PAQU-AMFb ROSY_AMF_58 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673781 ROSY_AMF_59 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625355 59WIN-VIOL-AMFb ROSY_AMF_59 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673782 ROSY_AMF_7 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625356 7CANEY-VIOL-AMFb ROSY_AMF_7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673783 ROSY_AMF_8 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625357 8DUD-LACT-AMFb ROSY_AMF_8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673784 ROSY_AMF_9 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625360 9DUD-PANI-AMFb ROSY_AMF_9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX12673785 ROSY_AMF_10 SRP341930 bp0 We extracted DNA from 0.25g of frozen roots from each site at each time point using the Qiagen DNeasy PowerPlant kit (Qiagen, Germantown, MD, USA). DNA was quantified fluorometrically (Qubit, Invitrogen, Carlsbad, CA, USA) and normalized to 20 ng/l for subsequent PCR. For PCR, we used Illumina TruSeq V3 indices (Illumina, San Diego, CA, USA) linked to AM fungal rDNA fungal-specific primers. These primers allow for the detection of the largest swath of AM fungi while also restricting non-target taxa (e.g., plants and animals). Reactions contained 20.5 l of platinum PCR Supermix (Invitrogen, Carlsbad, CA, USA), 1.25 l of each primer (10M), 0.5 l of BSA (20mg/mL), and 2 l of DNA. All PCRs were performed in triplicate with a hot start at 94 C for 3 min, and 25 cycles of 94 C for 45s, 50 C for 1 min, 72 C for 90 s, and a final extension step of 72 C for 10 min. Triplicate PCRs were combined and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and quantitated with a Qubit fluorometer. Samples were pooled in equal amounts and sequenced on Illumina MiSeq v3 (2 x 250bp PE run) at the Center for Environmental Biotechnology sequencing core at the University of Tennessee, Knoxville. SRS10625359 10DUD-PLMA-AMFb ROSY_AMF_10 AMPLICON METAGENOMIC PCR Illumina MiSeq