SRX12677169 GSM5631527_r1 SRP341965 GSE186076 SRS10628739 GSM5631527 GSM5631527 RNA-Seq TRANSCRIPTOMIC cDNA 40-45 Brains from 7-10 day old flies expressing gfp in ~15 R5 ellipsoid body neurons in morning or evening +/- sleep deprivation conditions were dissected. Flourescent-activated cell sorting (FACS) was used to isolate individual cells. Total RNA was extracted using PicoPure kit and cDNA was created using a T7-oligo-dT primer and SuperScript III and served as a template to make and amplify new mRNA using T7 RNA polymerase and the Ambion MegaScript kit . Libraries were generated using an Illumina TruSeq Stranded Kit Illumina NovaSeq 6000 SRX12677168 GSM5631526_r1 SRP341965 GSE186076 SRS10628742 GSM5631526 GSM5631526 RNA-Seq TRANSCRIPTOMIC cDNA 40-45 Brains from 7-10 day old flies expressing gfp in ~15 R5 ellipsoid body neurons in morning or evening +/- sleep deprivation conditions were dissected. Flourescent-activated cell sorting (FACS) was used to isolate individual cells. Total RNA was extracted using PicoPure kit and cDNA was created using a T7-oligo-dT primer and SuperScript III and served as a template to make and amplify new mRNA using T7 RNA polymerase and the Ambion MegaScript kit . Libraries were generated using an Illumina TruSeq Stranded Kit Illumina NovaSeq 6000 SRX12677167 GSM5631525_r1 SRP341965 GSE186076 SRS10628738 GSM5631525 GSM5631525 RNA-Seq TRANSCRIPTOMIC cDNA 40-45 Brains from 7-10 day old flies expressing gfp in ~15 R5 ellipsoid body neurons in morning or evening +/- sleep deprivation conditions were dissected. Flourescent-activated cell sorting (FACS) was used to isolate individual cells. Total RNA was extracted using PicoPure kit and cDNA was created using a T7-oligo-dT primer and SuperScript III and served as a template to make and amplify new mRNA using T7 RNA polymerase and the Ambion MegaScript kit . Libraries were generated using an Illumina TruSeq Stranded Kit Illumina NovaSeq 6000 SRX12677170 GSM5631528_r1 SRP341965 GSE186076 SRS10628741 GSM5631528 GSM5631528 RNA-Seq TRANSCRIPTOMIC cDNA 40-45 Brains from 7-10 day old flies expressing gfp in ~15 R5 ellipsoid body neurons in morning or evening +/- sleep deprivation conditions were dissected. Flourescent-activated cell sorting (FACS) was used to isolate individual cells. Total RNA was extracted using PicoPure kit and cDNA was created using a T7-oligo-dT primer and SuperScript III and served as a template to make and amplify new mRNA using T7 RNA polymerase and the Ambion MegaScript kit . Libraries were generated using an Illumina TruSeq Stranded Kit Illumina NovaSeq 6000 SRX12677171 GSM5631529_r1 SRP341965 GSE186076 SRS10628740 GSM5631529 GSM5631529 RNA-Seq TRANSCRIPTOMIC cDNA 40-45 Brains from 7-10 day old flies expressing gfp in ~15 R5 ellipsoid body neurons in morning or evening +/- sleep deprivation conditions were dissected. Flourescent-activated cell sorting (FACS) was used to isolate individual cells. Total RNA was extracted using PicoPure kit and cDNA was created using a T7-oligo-dT primer and SuperScript III and served as a template to make and amplify new mRNA using T7 RNA polymerase and the Ambion MegaScript kit . Libraries were generated using an Illumina TruSeq Stranded Kit Illumina NovaSeq 6000 SRX12677172 GSM5631530_r1 SRP341965 GSE186076 SRS10628743 GSM5631530 GSM5631530 RNA-Seq TRANSCRIPTOMIC cDNA 40-45 Brains from 7-10 day old flies expressing gfp in ~15 R5 ellipsoid body neurons in morning or evening +/- sleep deprivation conditions were dissected. Flourescent-activated cell sorting (FACS) was used to isolate individual cells. Total RNA was extracted using PicoPure kit and cDNA was created using a T7-oligo-dT primer and SuperScript III and served as a template to make and amplify new mRNA using T7 RNA polymerase and the Ambion MegaScript kit . Libraries were generated using an Illumina TruSeq Stranded Kit Illumina NovaSeq 6000 SRX12677174 GSM5631532_r1 SRP341965 GSE186076 SRS10628745 GSM5631532 GSM5631532 RNA-Seq TRANSCRIPTOMIC cDNA 40-45 Brains from 7-10 day old flies expressing gfp in ~15 R5 ellipsoid body neurons in morning or evening +/- sleep deprivation conditions were dissected. Flourescent-activated cell sorting (FACS) was used to isolate individual cells. Total RNA was extracted using PicoPure kit and cDNA was created using a T7-oligo-dT primer and SuperScript III and served as a template to make and amplify new mRNA using T7 RNA polymerase and the Ambion MegaScript kit . Libraries were generated using an Illumina TruSeq Stranded Kit Illumina NovaSeq 6000 SRX12677175 GSM5631533_r1 SRP341965 GSE186076 SRS10628746 GSM5631533 GSM5631533 RNA-Seq TRANSCRIPTOMIC cDNA 40-45 Brains from 7-10 day old flies expressing gfp in ~15 R5 ellipsoid body neurons in morning or evening +/- sleep deprivation conditions were dissected. Flourescent-activated cell sorting (FACS) was used to isolate individual cells. Total RNA was extracted using PicoPure kit and cDNA was created using a T7-oligo-dT primer and SuperScript III and served as a template to make and amplify new mRNA using T7 RNA polymerase and the Ambion MegaScript kit . Libraries were generated using an Illumina TruSeq Stranded Kit Illumina NovaSeq 6000 SRX12677173 GSM5631531_r1 SRP341965 GSE186076 SRS10628744 GSM5631531 GSM5631531 RNA-Seq TRANSCRIPTOMIC cDNA 40-45 Brains from 7-10 day old flies expressing gfp in ~15 R5 ellipsoid body neurons in morning or evening +/- sleep deprivation conditions were dissected. Flourescent-activated cell sorting (FACS) was used to isolate individual cells. Total RNA was extracted using PicoPure kit and cDNA was created using a T7-oligo-dT primer and SuperScript III and served as a template to make and amplify new mRNA using T7 RNA polymerase and the Ambion MegaScript kit . Libraries were generated using an Illumina TruSeq Stranded Kit Illumina NovaSeq 6000 SRX12677177 GSM5631535_r1 SRP341965 GSE186076 SRS10628747 GSM5631535 GSM5631535 RNA-Seq TRANSCRIPTOMIC cDNA 40-45 Brains from 7-10 day old flies expressing gfp in ~15 R5 ellipsoid body neurons in morning or evening +/- sleep deprivation conditions were dissected. Flourescent-activated cell sorting (FACS) was used to isolate individual cells. Total RNA was extracted using PicoPure kit and cDNA was created using a T7-oligo-dT primer and SuperScript III and served as a template to make and amplify new mRNA using T7 RNA polymerase and the Ambion MegaScript kit . Libraries were generated using an Illumina TruSeq Stranded Kit Illumina NovaSeq 6000 SRX12677178 GSM5631536_r1 SRP341965 GSE186076 SRS10628748 GSM5631536 GSM5631536 RNA-Seq TRANSCRIPTOMIC cDNA 40-45 Brains from 7-10 day old flies expressing gfp in ~15 R5 ellipsoid body neurons in morning or evening +/- sleep deprivation conditions were dissected. Flourescent-activated cell sorting (FACS) was used to isolate individual cells. Total RNA was extracted using PicoPure kit and cDNA was created using a T7-oligo-dT primer and SuperScript III and served as a template to make and amplify new mRNA using T7 RNA polymerase and the Ambion MegaScript kit . Libraries were generated using an Illumina TruSeq Stranded Kit Illumina NovaSeq 6000 SRX12677176 GSM5631534_r1 SRP341965 GSE186076 SRS10628749 GSM5631534 GSM5631534 RNA-Seq TRANSCRIPTOMIC cDNA 40-45 Brains from 7-10 day old flies expressing gfp in ~15 R5 ellipsoid body neurons in morning or evening +/- sleep deprivation conditions were dissected. Flourescent-activated cell sorting (FACS) was used to isolate individual cells. Total RNA was extracted using PicoPure kit and cDNA was created using a T7-oligo-dT primer and SuperScript III and served as a template to make and amplify new mRNA using T7 RNA polymerase and the Ambion MegaScript kit . Libraries were generated using an Illumina TruSeq Stranded Kit Illumina NovaSeq 6000