SRX12678048 GSM5631661 SRP341991 SRS10629542 GSM5631661 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631661 GSM5631661 GEO Accession GSM5631661 SRX12678049 GSM5631662 SRP341991 SRS10629543 GSM5631662 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631662 GSM5631662 GEO Accession GSM5631662 SRX12678050 GSM5631663 SRP341991 SRS10629544 GSM5631663 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631663 GSM5631663 GEO Accession GSM5631663 SRX12678051 GSM5631664 SRP341991 SRS10629545 GSM5631664 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631664 GSM5631664 GEO Accession GSM5631664 SRX12678052 GSM5631665 SRP341991 SRS10629546 GSM5631665 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631665 GSM5631665 GEO Accession GSM5631665 SRX12678053 GSM5631666 SRP341991 SRS10629547 GSM5631666 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631666 GSM5631666 GEO Accession GSM5631666 SRX12678054 GSM5631667 SRP341991 SRS10629548 GSM5631667 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631667 GSM5631667 GEO Accession GSM5631667 SRX12678055 GSM5631668 SRP341991 SRS10629549 GSM5631668 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631668 GSM5631668 GEO Accession GSM5631668 SRX12678056 GSM5631669 SRP341991 SRS10629550 GSM5631669 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631669 GSM5631669 GEO Accession GSM5631669 SRX12678057 GSM5631670 SRP341991 SRS10629551 GSM5631670 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631670 GSM5631670 GEO Accession GSM5631670 SRX12678058 GSM5631671 SRP341991 SRS10629552 GSM5631671 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631671 GSM5631671 GEO Accession GSM5631671 SRX12678059 GSM5631672 SRP341991 SRS10629553 GSM5631672 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631672 GSM5631672 GEO Accession GSM5631672 SRX12678060 GSM5631673 SRP341991 SRS10629554 GSM5631673 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631673 GSM5631673 GEO Accession GSM5631673 SRX12678061 GSM5631674 SRP341991 SRS10629555 GSM5631674 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631674 GSM5631674 GEO Accession GSM5631674 SRX12678062 GSM5631675 SRP341991 SRS10629556 GSM5631675 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631675 GSM5631675 GEO Accession GSM5631675 SRX12678063 GSM5631676 SRP341991 SRS10629557 GSM5631676 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631676 GSM5631676 GEO Accession GSM5631676 SRX12678064 GSM5631628 SRP341991 SRS10629559 GSM5631628 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631628 GSM5631628 GEO Accession GSM5631628 SRX12678065 GSM5631677 SRP341991 SRS10629558 GSM5631677 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631677 GSM5631677 GEO Accession GSM5631677 SRX12678066 GSM5631678 SRP341991 SRS10629561 GSM5631678 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631678 GSM5631678 GEO Accession GSM5631678 SRX12678067 GSM5631679 SRP341991 SRS10629560 GSM5631679 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631679 GSM5631679 GEO Accession GSM5631679 SRX12678068 GSM5631680 SRP341991 SRS10629562 GSM5631680 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631680 GSM5631680 GEO Accession GSM5631680 SRX12678069 GSM5631681 SRP341991 SRS10629563 GSM5631681 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631681 GSM5631681 GEO Accession GSM5631681 SRX12678070 GSM5631682 SRP341991 SRS10629564 GSM5631682 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631682 GSM5631682 GEO Accession GSM5631682 SRX12678071 GSM5631683 SRP341991 SRS10629565 GSM5631683 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631683 GSM5631683 GEO Accession GSM5631683 SRX12678072 GSM5631684 SRP341991 SRS10629567 GSM5631684 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631684 GSM5631684 GEO Accession GSM5631684 SRX12678073 GSM5631685 SRP341991 SRS10629566 GSM5631685 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631685 GSM5631685 GEO Accession GSM5631685 SRX12678074 GSM5631686 SRP341991 SRS10629568 GSM5631686 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631686 GSM5631686 GEO Accession GSM5631686 SRX12678075 GSM5631687 SRP341991 SRS10629569 GSM5631687 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631687 GSM5631687 GEO Accession GSM5631687 SRX12678076 GSM5631688 SRP341991 SRS10629570 GSM5631688 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631688 GSM5631688 GEO Accession GSM5631688 SRX12678077 GSM5631689 SRP341991 SRS10629572 GSM5631689 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631689 GSM5631689 GEO Accession GSM5631689 SRX12678078 GSM5631690 SRP341991 SRS10629571 GSM5631690 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631690 GSM5631690 GEO Accession GSM5631690 SRX12678079 GSM5631691 SRP341991 SRS10629573 GSM5631691 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631691 GSM5631691 GEO Accession GSM5631691 SRX12678080 GSM5631692 SRP341991 SRS10629575 GSM5631692 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631692 GSM5631692 GEO Accession GSM5631692 SRX12678081 GSM5631693 SRP341991 SRS10629574 GSM5631693 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631693 GSM5631693 GEO Accession GSM5631693 SRX12678082 GSM5631694 SRP341991 SRS10629576 GSM5631694 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631694 GSM5631694 GEO Accession GSM5631694 SRX12678083 GSM5631695 SRP341991 SRS10629577 GSM5631695 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631695 GSM5631695 GEO Accession GSM5631695 SRX12678084 GSM5631696 SRP341991 SRS10629578 GSM5631696 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631696 GSM5631696 GEO Accession GSM5631696 SRX12678085 GSM5631697 SRP341991 SRS10629579 GSM5631697 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631697 GSM5631697 GEO Accession GSM5631697 SRX12678086 GSM5631698 SRP341991 SRS10629580 GSM5631698 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631698 GSM5631698 GEO Accession GSM5631698 SRX12678087 GSM5631699 SRP341991 SRS10629581 GSM5631699 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631699 GSM5631699 GEO Accession GSM5631699 SRX12678088 GSM5631700 SRP341991 SRS10629582 GSM5631700 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631700 GSM5631700 GEO Accession GSM5631700 SRX12678089 GSM5631701 SRP341991 SRS10629583 GSM5631701 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631701 GSM5631701 GEO Accession GSM5631701 SRX12678090 GSM5631702 SRP341991 SRS10629585 GSM5631702 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631702 GSM5631702 GEO Accession GSM5631702 SRX12678091 GSM5631703 SRP341991 SRS10629584 GSM5631703 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631703 GSM5631703 GEO Accession GSM5631703 SRX12678092 GSM5631704 SRP341991 SRS10629586 GSM5631704 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631704 GSM5631704 GEO Accession GSM5631704 SRX12678093 GSM5631705 SRP341991 SRS10629587 GSM5631705 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631705 GSM5631705 GEO Accession GSM5631705 SRX12678094 GSM5631629 SRP341991 SRS10629588 GSM5631629 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631629 GSM5631629 GEO Accession GSM5631629 SRX12678095 GSM5631630 SRP341991 SRS10629589 GSM5631630 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631630 GSM5631630 GEO Accession GSM5631630 SRX12678096 GSM5631631 SRP341991 SRS10629590 GSM5631631 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631631 GSM5631631 GEO Accession GSM5631631 SRX12678097 GSM5631632 SRP341991 SRS10629591 GSM5631632 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631632 GSM5631632 GEO Accession GSM5631632 SRX12678098 GSM5631633 SRP341991 SRS10629592 GSM5631633 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631633 GSM5631633 GEO Accession GSM5631633 SRX12678099 GSM5631634 SRP341991 SRS10629593 GSM5631634 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631634 GSM5631634 GEO Accession GSM5631634 SRX12678100 GSM5631635 SRP341991 SRS10629594 GSM5631635 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631635 GSM5631635 GEO Accession GSM5631635 SRX12678101 GSM5631636 SRP341991 SRS10629595 GSM5631636 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631636 GSM5631636 GEO Accession GSM5631636 SRX12678102 GSM5631637 SRP341991 SRS10629596 GSM5631637 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631637 GSM5631637 GEO Accession GSM5631637 SRX12678103 GSM5631638 SRP341991 SRS10629597 GSM5631638 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631638 GSM5631638 GEO Accession GSM5631638 SRX12678104 GSM5631639 SRP341991 SRS10629598 GSM5631639 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631639 GSM5631639 GEO Accession GSM5631639 SRX12678105 GSM5631640 SRP341991 SRS10629599 GSM5631640 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631640 GSM5631640 GEO Accession GSM5631640 SRX12678106 GSM5631641 SRP341991 SRS10629600 GSM5631641 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631641 GSM5631641 GEO Accession GSM5631641 SRX12678107 GSM5631642 SRP341991 SRS10629601 GSM5631642 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631642 GSM5631642 GEO Accession GSM5631642 SRX12678108 GSM5631643 SRP341991 SRS10629602 GSM5631643 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631643 GSM5631643 GEO Accession GSM5631643 SRX12678109 GSM5631644 SRP341991 SRS10629603 GSM5631644 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631644 GSM5631644 GEO Accession GSM5631644 SRX12678110 GSM5631645 SRP341991 SRS10629604 GSM5631645 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631645 GSM5631645 GEO Accession GSM5631645 SRX12678111 GSM5631646 SRP341991 SRS10629605 GSM5631646 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631646 GSM5631646 GEO Accession GSM5631646 SRX12678112 GSM5631647 SRP341991 SRS10629606 GSM5631647 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631647 GSM5631647 GEO Accession GSM5631647 SRX12678113 GSM5631648 SRP341991 SRS10629607 GSM5631648 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631648 GSM5631648 GEO Accession GSM5631648 SRX12678114 GSM5631649 SRP341991 SRS10629608 GSM5631649 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631649 GSM5631649 GEO Accession GSM5631649 SRX12678115 GSM5631650 SRP341991 SRS10629609 GSM5631650 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631650 GSM5631650 GEO Accession GSM5631650 SRX12678116 GSM5631651 SRP341991 SRS10629610 GSM5631651 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631651 GSM5631651 GEO Accession GSM5631651 SRX12678117 GSM5631652 SRP341991 SRS10629611 GSM5631652 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631652 GSM5631652 GEO Accession GSM5631652 SRX12678118 GSM5631653 SRP341991 SRS10629612 GSM5631653 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631653 GSM5631653 GEO Accession GSM5631653 SRX12678119 GSM5631654 SRP341991 SRS10629613 GSM5631654 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631654 GSM5631654 GEO Accession GSM5631654 SRX12678120 GSM5631655 SRP341991 SRS10629614 GSM5631655 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631655 GSM5631655 GEO Accession GSM5631655 SRX12678121 GSM5631656 SRP341991 SRS10629615 GSM5631656 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631656 GSM5631656 GEO Accession GSM5631656 SRX12678122 GSM5631657 SRP341991 SRS10629616 GSM5631657 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631657 GSM5631657 GEO Accession GSM5631657 SRX12678123 GSM5631658 SRP341991 SRS10629617 GSM5631658 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631658 GSM5631658 GEO Accession GSM5631658 SRX12678124 GSM5631659 SRP341991 SRS10629618 GSM5631659 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631659 GSM5631659 GEO Accession GSM5631659 SRX12678125 GSM5631660 SRP341991 SRS10629619 GSM5631660 miRNA-Seq TRANSCRIPTOMIC size fractionation MicrobiomeInsight did the extraction. DNA is extracted from samples using the Qiagen MagAttract PowerSoil DNA KF Kit (Formerly MO BIO PowerSoil DNA Kit) optimized for the Thermofisher KingFisher robot. This kit uses magnetic beads to specifically capture DNA while excluding organic inhibitors. In-house testing has shown this kit to have a good balance of DNA yield and quality as demonstrated on a variety of environmental sample types. DNA quantification and quality checks are done via Qubit. The high-fidelity Phusion polymerase for amplification of marker genes will be used. If extractions contain some carry-over inhibition or a high concentration of DNA, typically1:1 and 1:10 dilutions will be tested and run the PCR products on gels for verification. PCR is done with dual-barcoded primers targeting either the prokaryotic 16S DNA with modified V4 skin cutaneous microbiome protocol. Samples with failed PCRs (or spurious bands) are re-amplified by optimizing PCR conditions. The PCR reactions are cleaned-up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified accurately with the KAPA qPCR Library Quant kit. Library pools are sequenced on the Illumina MiSeq using the corresponding primer sets for prokaryotic 16S V4_skin. Illumina MiSeq gds 305631660 GSM5631660 GEO Accession GSM5631660