SRP342023 PRJNA772550 Transcriptome profiling of Nicotiana tabacum CB-1 at five different maturity stages According to the morphological characteristics of leaf color,vein, and villus, five different maturity stages (M1,M2, M3, M4, and M5) of the middle leaves (eighth to 10th, counted from the bottom to the top) were collected in the same field as samples. Three biological replicates with each replicatecontaining three leaves from different plant were collected.