SRP342174 PRJNA772777 GSE186174 Salivary TIMP1 and predicted mir-141, possible transcripts biomarkers for estrus in the buffalo (Bubalus bubalis) RNA Seq data showed 49 differentially altered mRNAs in estrus and diestrus stages of buffalo cell free saliva, that includes 10 mRNAs commonly present in both estrus and diestrus stages, whereas 12 and 27 mRNAs were found to be exclusively present either at the estrus or the diestrus stage respectively. In addition, four mature miRNAs were predicted to be differentially altered in the estrus as compared to the diestrus phase of buffaloes. Overall design: Differentially expressed transcripts were identified in estrus as compared to diestrus phase in buffaloes GSE186174 pubmed 35525172