SRP342184 PRJNA772790 RNA-sequencing of invariant NKT cells from spleen, liver and adipose tissue Invariant NKT cells show distinct immunometabolic features in the spleen, liver and adipose tissue. Hepatic and splenic iNKTs rely on glycolytic metabolism and AT iNKTs depend on mitochondrial fatty acid metabolism. Disruption of key metabolic enzymes affects their function in liver injury and obesity-induced inflammation.