SRX12708394 p1 SRP342406 PRJNA772272 Splenocytes were obtained from 8 week-old mice by homogenizing splenic tissue, passing through a 40 m filter and rinsing with PBS + 10% FBS. RBCs were lysed and the remaining mononuclear cells were cryopreserved in PBS with 10% DMSO and 10% FCS. Cells were formaldehyde cross-linked, digested into approximately. 0.2 kb fragments by NlaIII and ligated to form larger circular DNA segments that include regions of neighboring DNA. The circular DNA was then de- crosslinked and digested with NspI to form 2kb fragments of DNA.31 PCR primer pairs were designed to each of the three transgenic fragments and used to selectively amplify and sequence regions of DNA that were near to the transgenic fragments of interest within the Berkeley mouse genome. SRS10628375 SAMN22374110 p1 OTHER GENOMIC PCR Illumina MiSeq SRX12708395 p2 SRP342406 PRJNA772272 Splenocytes were obtained from 8 week-old mice by homogenizing splenic tissue, passing through a 40 m filter and rinsing with PBS + 10% FBS. RBCs were lysed and the remaining mononuclear cells were cryopreserved in PBS with 10% DMSO and 10% FCS. Cells were formaldehyde cross-linked, digested into approximately. 0.2 kb fragments by NlaIII and ligated to form larger circular DNA segments that include regions of neighboring DNA. The circular DNA was then de- crosslinked and digested with NspI to form 2kb fragments of DNA.31 PCR primer pairs were designed to each of the three transgenic fragments and used to selectively amplify and sequence regions of DNA that were near to the transgenic fragments of interest within the Berkeley mouse genome. SRS10628375 SAMN22374110 p2 OTHER GENOMIC PCR Illumina MiSeq SRX12708396 p3 SRP342406 PRJNA772272 Splenocytes were obtained from 8 week-old mice by homogenizing splenic tissue, passing through a 40 m filter and rinsing with PBS + 10% FBS. RBCs were lysed and the remaining mononuclear cells were cryopreserved in PBS with 10% DMSO and 10% FCS. Cells were formaldehyde cross-linked, digested into approximately. 0.2 kb fragments by NlaIII and ligated to form larger circular DNA segments that include regions of neighboring DNA. The circular DNA was then de- crosslinked and digested with NspI to form 2kb fragments of DNA.31 PCR primer pairs were designed to each of the three transgenic fragments and used to selectively amplify and sequence regions of DNA that were near to the transgenic fragments of interest within the Berkeley mouse genome. SRS10628375 SAMN22374110 p3 OTHER GENOMIC PCR Illumina MiSeq SRX12708397 p4 SRP342406 PRJNA772272 Splenocytes were obtained from 8 week-old mice by homogenizing splenic tissue, passing through a 40 m filter and rinsing with PBS + 10% FBS. RBCs were lysed and the remaining mononuclear cells were cryopreserved in PBS with 10% DMSO and 10% FCS. Cells were formaldehyde cross-linked, digested into approximately. 0.2 kb fragments by NlaIII and ligated to form larger circular DNA segments that include regions of neighboring DNA. The circular DNA was then de- crosslinked and digested with NspI to form 2kb fragments of DNA.31 PCR primer pairs were designed to each of the three transgenic fragments and used to selectively amplify and sequence regions of DNA that were near to the transgenic fragments of interest within the Berkeley mouse genome. SRS10628375 SAMN22374110 p4 OTHER GENOMIC PCR Illumina MiSeq SRX12708398 p5 SRP342406 PRJNA772272 Splenocytes were obtained from 8 week-old mice by homogenizing splenic tissue, passing through a 40 m filter and rinsing with PBS + 10% FBS. RBCs were lysed and the remaining mononuclear cells were cryopreserved in PBS with 10% DMSO and 10% FCS. Cells were formaldehyde cross-linked, digested into approximately. 0.2 kb fragments by NlaIII and ligated to form larger circular DNA segments that include regions of neighboring DNA. The circular DNA was then de- crosslinked and digested with NspI to form 2kb fragments of DNA.31 PCR primer pairs were designed to each of the three transgenic fragments and used to selectively amplify and sequence regions of DNA that were near to the transgenic fragments of interest within the Berkeley mouse genome. SRS10628375 SAMN22374110 p5 OTHER GENOMIC PCR Illumina MiSeq SRX12708399 p6 SRP342406 PRJNA772272 Splenocytes were obtained from 8 week-old mice by homogenizing splenic tissue, passing through a 40 m filter and rinsing with PBS + 10% FBS. RBCs were lysed and the remaining mononuclear cells were cryopreserved in PBS with 10% DMSO and 10% FCS. Cells were formaldehyde cross-linked, digested into approximately. 0.2 kb fragments by NlaIII and ligated to form larger circular DNA segments that include regions of neighboring DNA. The circular DNA was then de- crosslinked and digested with NspI to form 2kb fragments of DNA.31 PCR primer pairs were designed to each of the three transgenic fragments and used to selectively amplify and sequence regions of DNA that were near to the transgenic fragments of interest within the Berkeley mouse genome. SRS10628375 SAMN22374110 p6 OTHER GENOMIC PCR Illumina MiSeq