SRX12739912 GSM5649873_r1 SRP342714 PRJNA773723 SRS10688156 GSM5649873 GSM5649873 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739913 GSM5649874_r1 SRP342714 PRJNA773723 SRS10688157 GSM5649874 GSM5649874 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739914 GSM5649875_r1 SRP342714 PRJNA773723 SRS10688158 GSM5649875 GSM5649875 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739915 GSM5649876_r1 SRP342714 PRJNA773723 SRS10688159 GSM5649876 GSM5649876 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739916 GSM5649877_r1 SRP342714 PRJNA773723 SRS10688160 GSM5649877 GSM5649877 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739917 GSM5649878_r1 SRP342714 PRJNA773723 SRS10688161 GSM5649878 GSM5649878 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739918 GSM5649879_r1 SRP342714 PRJNA773723 SRS10688162 GSM5649879 GSM5649879 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739919 GSM5649880_r1 SRP342714 PRJNA773723 SRS10688163 GSM5649880 GSM5649880 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739920 GSM5649881_r1 SRP342714 PRJNA773723 SRS10688164 GSM5649881 GSM5649881 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739921 GSM5649882_r1 SRP342714 PRJNA773723 SRS10688165 GSM5649882 GSM5649882 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739922 GSM5649883_r1 SRP342714 PRJNA773723 SRS10688166 GSM5649883 GSM5649883 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739923 GSM5649884_r1 SRP342714 PRJNA773723 SRS10688167 GSM5649884 GSM5649884 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739924 GSM5649885_r1 SRP342714 PRJNA773723 SRS10688168 GSM5649885 GSM5649885 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739925 GSM5649886_r1 SRP342714 PRJNA773723 SRS10688169 GSM5649886 GSM5649886 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739926 GSM5649887_r1 SRP342714 PRJNA773723 SRS10688170 GSM5649887 GSM5649887 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739927 GSM5649888_r1 SRP342714 PRJNA773723 SRS10688171 GSM5649888 GSM5649888 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739928 GSM5649889_r1 SRP342714 PRJNA773723 SRS10688172 GSM5649889 GSM5649889 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739929 GSM5649890_r1 SRP342714 PRJNA773723 SRS10688173 GSM5649890 GSM5649890 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739930 GSM5649891_r1 SRP342714 PRJNA773723 SRS10688174 GSM5649891 GSM5649891 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739931 GSM5649892_r1 SRP342714 PRJNA773723 SRS10688175 GSM5649892 GSM5649892 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739932 GSM5649893_r1 SRP342714 PRJNA773723 SRS10688176 GSM5649893 GSM5649893 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739933 GSM5649894_r1 SRP342714 PRJNA773723 SRS10688177 GSM5649894 GSM5649894 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739934 GSM5649895_r1 SRP342714 PRJNA773723 SRS10688178 GSM5649895 GSM5649895 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739935 GSM5649896_r1 SRP342714 PRJNA773723 SRS10688179 GSM5649896 GSM5649896 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739936 GSM5649897_r1 SRP342714 PRJNA773723 SRS10688180 GSM5649897 GSM5649897 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739937 GSM5649898_r1 SRP342714 PRJNA773723 SRS10688181 GSM5649898 GSM5649898 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739938 GSM5649899_r1 SRP342714 PRJNA773723 SRS10688182 GSM5649899 GSM5649899 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739939 GSM5649900_r1 SRP342714 PRJNA773723 SRS10688183 GSM5649900 GSM5649900 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739940 GSM5649901_r1 SRP342714 PRJNA773723 SRS10688184 GSM5649901 GSM5649901 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000 SRX12739941 GSM5649902_r1 SRP342714 PRJNA773723 SRS10688185 GSM5649902 GSM5649902 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). RT-PCR assays were performed using the OneStep RT-PCR kit (QIAGEN), as per the manufacturer's instructions. 1000 ng per sample was used in the library preparation. To obtain larger insert size (540 nt on average after size selection), Illumina's TruSeq Stranded mRNA sample preparation protocol was modified as follows: 1) fragmentation time and temperature was reduced from 8 minutes at 98 C to 2 minutes at 80 C. Average size distribution prior to library preparation was 232 nt versus 740 nt, respectively; 2) after the final library was generated, the samples were loaded onto a 2% agarose gel (Cat # 1613101, BioRad) and run for 1 hour at 100 V. The libraries were size selected between 400 and 600 bp. Excised gel fragments were purified using the Qiagen MinElute Gel Extraction kit (Cat # 28604, Qiagen). The rest of the protocol was adapted as described in Illumina's TruSeq Stranded mRNA sample preparation guide (Part# 15031047 Rev. E, Illumina). Illumina NovaSeq 6000