SRX12797718 GSM5658565 SRP343396 SRS10743652 GSM5658565 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658565 GSM5658565 GEO Accession GSM5658565 SRX12797719 GSM5658566 SRP343396 SRS10743653 GSM5658566 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658566 GSM5658566 GEO Accession GSM5658566 SRX12797720 GSM5658567 SRP343396 SRS10743654 GSM5658567 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658567 GSM5658567 GEO Accession GSM5658567 SRX12797721 GSM5658568 SRP343396 SRS10743655 GSM5658568 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658568 GSM5658568 GEO Accession GSM5658568 SRX12797722 GSM5658569 SRP343396 SRS10743656 GSM5658569 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658569 GSM5658569 GEO Accession GSM5658569 SRX12797723 GSM5658570 SRP343396 SRS10743657 GSM5658570 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658570 GSM5658570 GEO Accession GSM5658570 SRX12797724 GSM5658571 SRP343396 SRS10743659 GSM5658571 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658571 GSM5658571 GEO Accession GSM5658571 SRX12797725 GSM5658572 SRP343396 SRS10743658 GSM5658572 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658572 GSM5658572 GEO Accession GSM5658572 SRX12797726 GSM5658573 SRP343396 SRS10743660 GSM5658573 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658573 GSM5658573 GEO Accession GSM5658573 SRX12797727 GSM5658574 SRP343396 SRS10743661 GSM5658574 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658574 GSM5658574 GEO Accession GSM5658574 SRX12797728 GSM5658575 SRP343396 SRS10743662 GSM5658575 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658575 GSM5658575 GEO Accession GSM5658575 SRX12797729 GSM5658576 SRP343396 SRS10743663 GSM5658576 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658576 GSM5658576 GEO Accession GSM5658576 SRX12797730 GSM5658577 SRP343396 SRS10743664 GSM5658577 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658577 GSM5658577 GEO Accession GSM5658577 SRX12797731 GSM5658578 SRP343396 SRS10743665 GSM5658578 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658578 GSM5658578 GEO Accession GSM5658578 SRX12797732 GSM5658579 SRP343396 SRS10743666 GSM5658579 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658579 GSM5658579 GEO Accession GSM5658579 SRX12797733 GSM5658580 SRP343396 SRS10743667 GSM5658580 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658580 GSM5658580 GEO Accession GSM5658580 SRX12797734 GSM5658581 SRP343396 SRS10743668 GSM5658581 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658581 GSM5658581 GEO Accession GSM5658581 SRX12797735 GSM5658582 SRP343396 SRS10743669 GSM5658582 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658582 GSM5658582 GEO Accession GSM5658582 SRX12797736 GSM5658583 SRP343396 SRS10743670 GSM5658583 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658583 GSM5658583 GEO Accession GSM5658583 SRX12797737 GSM5658584 SRP343396 SRS10743671 GSM5658584 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658584 GSM5658584 GEO Accession GSM5658584 SRX12797738 GSM5658585 SRP343396 SRS10743672 GSM5658585 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658585 GSM5658585 GEO Accession GSM5658585 SRX12797739 GSM5658586 SRP343396 SRS10743673 GSM5658586 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658586 GSM5658586 GEO Accession GSM5658586 SRX12797740 GSM5658587 SRP343396 SRS10743674 GSM5658587 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658587 GSM5658587 GEO Accession GSM5658587 SRX12797741 GSM5658588 SRP343396 SRS10743675 GSM5658588 RNA-Seq TRANSCRIPTOMIC cDNA Gravid adults were rinsed off the plates with M9 + 0.025% Triton, washed 5x to get rid of offspring, mixed with TRIzol reagent (Invitrogen), and snap-frozen in liquid nitrogen. After breaking open the worms by 5-7 thaw-freeze cycles total RNA was isolated using the NucleoSpin miRNA extraction kit, protocol 5.2 (Macherey-Nagel). RNA sequencing libraries were prepared from total RNA according to standard Illumina protocols using the TruSeq RNA Library Kit v2, with indexed adaptors for multiplex sequencing, and submitted for 100 bp paired-end sequencing on a NovaSeq 6000. Illumina NovaSeq 6000 gds 305658588 GSM5658588 GEO Accession GSM5658588