SRX12797945 Alydus calcaratus CMF0925 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743734 Alydus calcaratus CMF0925 Alydus calcaratus CMF0925 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797946 Anoplocerus luteus CMF0989 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743735 Anoplocerus luteus CMF0989 Anoplocerus luteus CMF0989 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797947 Clavigralla elongata CMF0944 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743736 Clavigralla elongata CMF0944 Clavigralla elongata CMF0944 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797948 Clavigralla minor CMF0334 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743737 Clavigralla minor CMF0334 Clavigralla minor CMF0334 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797949 Clavigralla pusilla CMF0952-0960 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743738 Clavigralla pusilla CMF0952-0960 Clavigralla pusilla CMF0952-0960 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797950 Clavigralla shadabi CMF0056 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743739 Clavigralla shadabi CMF0056 Clavigralla shadabi CMF0056 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797951 Clavigralloides acantharis CMF0797 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743740 Clavigralloides acantharis CMF0797 Clavigralloides acantharis CMF0797 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797952 Coriomeris affinis CMF0935 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743741 Coriomeris affinis CMF0935 Coriomeris affinis CMF0935 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797953 Coriomeris denticulatus CMF0979 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743742 Coriomeris denticulatus CMF0979 Coriomeris denticulatus CMF0979 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797954 Coriomeris nigricornis CMF0560 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743743 Coriomeris nigricornis CMF0560 Coriomeris nigricornis CMF0560 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797955 Corizus hyoscyami CMF0973 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743744 Corizus hyoscyami CMF0973 Corizus hyoscyami CMF0973 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797956 Dulichius trispinosus CMF0906 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743745 Dulichius trispinosus CMF0906 Dulichius trispinosus CMF0906 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797957 Apidaurus conspersus CMF0900 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743746 Apidaurus conspersus CMF0900 Apidaurus conspersus CMF0900 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797958 Gralliclava horrens CMF1165 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743747 Gralliclava horrens CMF1165 Gralliclava horrens CMF1165 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797959 Gralliclava sp. CMF0745 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743748 Gralliclava sp. CMF0745 Gralliclava sp. CMF0745 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797960 Heegeria tangirica CMF0961 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743749 Heegeria tangirica CMF0961 Heegeria tangirica CMF0961 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797961 Hoplolomia scabricula CMF0857 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743750 Hoplolomia scabricula CMF0857 Hoplolomia scabricula CMF0857 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797962 Hyalymenus pulcher CMF0546 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743751 Hyalymenus pulcher CMF0546 Hyalymenus pulcher CMF0546 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797963 Hyalymenus sp.2 CMF0336 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743752 Hyalymenus sp.2 CMF0336 Hyalymenus sp.2 CMF0336 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797964 Hyalymenus sp.1 CMF1139 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743753 Hyalymenus sp.1 CMF1139 Hyalymenus sp.1 CMF1139 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797965 Hydarella chiangdaoensis CMF1129 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743754 Hydarella chiangdaoensis CMF1129 Hydarella chiangdaoensis CMF1129 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797966 Hydaropsis longirostris CMF0817 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743755 Hydaropsis longirostris CMF0817 Hydaropsis longirostris CMF0817 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797967 Leptocorisa bipunctata CMF0896 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743756 Leptocorisa bipunctata CMF0896 Leptocorisa bipunctata CMF0896 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797968 Arenocoris waltlii CMF0911 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743757 Arenocoris waltlii CMF0911 Arenocoris waltlii CMF0911 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797969 Liorhyssus hyalinus CMF0898 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743758 Liorhyssus hyalinus CMF0898 Liorhyssus hyalinus CMF0898 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797970 Maccevethus errans CMF0986 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743759 Maccevethus errans CMF0986 Maccevethus errans CMF0986 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797971 Madura fuscoclavata CMF1078 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743760 Madura fuscoclavata CMF1078 Madura fuscoclavata CMF1078 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797972 Maduranoides chemsaki CMF1017 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743761 Maduranoides chemsaki CMF1017 Maduranoides chemsaki CMF1017 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797973 Melanacanthus sp. CMF0416 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743762 Melanacanthus sp. CMF0416 Melanacanthus sp. CMF0416 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797974 Mevanidea hystrix CMF1088 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743763 Mevanidea hystrix CMF1088 Mevanidea hystrix CMF1088 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797975 Mevanidea spiniceps CMF0950-0953 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743764 Mevanidea spiniceps CMF0950-0953 Mevanidea spiniceps CMF0950-0953 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797976 Micrelytra fossularum CMF0910 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743765 Micrelytra fossularum CMF0910 Micrelytra fossularum CMF0910 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797977 Micrelytrini sp. CMF0161 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743766 Micrelytrini sp. CMF0161 Micrelytrini sp. CMF0161 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797978 Nariscus sp. CMF0908 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743767 Nariscus sp. CMF0908 Nariscus sp. CMF0908 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797979 Bathysolen nubilus CMF0602 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743768 Bathysolen nubilus CMF0602 Bathysolen nubilus CMF0602 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797980 Nemocoris falleni CMF0994 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743769 Nemocoris falleni CMF0994 Nemocoris falleni CMF0994 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797981 Paramyla suspecta CMF0807 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743770 Paramyla suspecta CMF0807 Paramyla suspecta CMF0807 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797982 Pseudomyla cornuta CMF1122 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743771 Pseudomyla cornuta CMF1122 Pseudomyla cornuta CMF1122 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797983 Psilolomia brunneofusca CMF0803 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743772 Psilolomia brunneofusca CMF0803 Psilolomia brunneofusca CMF0803 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797984 Psilolomia parva CMF0871 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743773 Psilolomia parva CMF0871 Psilolomia parva CMF0871 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797985 Riptortus sp. CMF0404 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743774 Riptortus sp. CMF0404 Riptortus sp. CMF0404 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797986 Stenocoris sp. CMF0315 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743775 Stenocoris sp. CMF0315 Stenocoris sp. CMF0315 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797987 Strobilotoma typhaecornis CMF0923 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743776 Strobilotoma typhaecornis CMF0923 Strobilotoma typhaecornis CMF0923 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797988 Tenosius sp. CMF0966 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743777 Tenosius sp. CMF0966 Tenosius sp. CMF0966 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797989 Vilga dallasi CMF1047 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743778 Vilga dallasi CMF1047 Vilga dallasi CMF1047 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797990 Burtinus luteomarginatus CMF0901 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743779 Burtinus luteomarginatus CMF0901 Burtinus luteomarginatus CMF0901 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797991 Vilga sanctipauli CMF1048 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743780 Vilga sanctipauli CMF1048 Vilga sanctipauli CMF1048 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797992 Vilga westwoodi CMF1050 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743781 Vilga westwoodi CMF1050 Vilga westwoodi CMF1050 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797993 Camptopus lateralis CMF0929 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743782 Camptopus lateralis CMF0929 Camptopus lateralis CMF0929 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797994 Ceraleptus gracilicornis CMF0988 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743783 Ceraleptus gracilicornis CMF0988 Ceraleptus gracilicornis CMF0988 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797995 Ceraleptus obtusus CMF0931 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743784 Ceraleptus obtusus CMF0931 Ceraleptus obtusus CMF0931 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000 SRX12797996 Chorosoma schillingii CMF0933 SRP343402 bp0 Genomic DNA was extracted using a 1) Gentra Puregene Tissue, 2) Qiagen DNeasy Blood and Tissue kit (see Forthman et al., 2019), or 3) Qiagen DNeasy Blood and Tissue kit coupled with Qiagen QIAquick PCR purification kit (see Knyschov et al., 2019; Forthman et al. 2019, 2020). Degraded DNA from pinned museum samples were repaired using a PreCR Repair Mix kit, with a 3X SPRI clean-up. Libraries were constructed from genomic DNA following Forthman et al. (2019). Cleaned, amplified libraries were quantified with Qubit and subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) and newly designed probes derived from coreoid transcriptomes were used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. For some samples, we followed Forthman et al. (2019) or Forthman et al. (2020) enrichment protocols, while others were subjected to a modified enrichment protocol: baits hybridized with library pools at 65C for 12 hours followed by 12 hours at 62C and then by 12 hours at 60C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 60C, and resuspended in 30 uL IDTE. 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14-17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Florida Interdisciplinary Center for Biotechnology Research. SRS10743785 Chorosoma schillingii CMF0933 Chorosoma schillingii CMF0933 Targeted-Capture GENOMIC Reduced Representation Illumina HiSeq 3000