SRP343777 PRJNA776292 GSE186852 Time-course transcriptome analysis-guided identification of a four-component system, SbrH1-R that regulates milbemycin biosynthesis by influencing gene cluster expression, precursor supply, and antibiotic efflux Streptomyces bingchenggensis is a soil bacterium that produces milbemycins, which have been applied widely as insecticides and anthelmintics in agricultural and veterinary field. To gain further insights into the regulatory mechanisms in Streptomyces bingchenggensis and mine useful targets for future metabolic engineering to improve milbemycin production, we have compared two strains(the parental strain and high-yielding strain)by comparative transcriptome analysis.This work provides beneficial targets and corresponding engineering methods to facilitate high-production of milbemycins and other polyketide pesticides. Overall design: 2 time points were designed at 3 and 6 day in two strains. The samples were collected at each time point named W1,W2,H1,H2 GSE186852