SRX13128816 GSM5689051 SRP345880 SRS11062776 GSM5689051 RIP-Seq TRANSCRIPTOMIC other The eCLIP-sequencing protocol described by Van Nostrand et al (Methods Mol. Biol., 2017, 1648, 177-200.) was applied with minor modifications. Twenty million cells were lysed in CLIP buffer (50 mM Tris-HCl pH 7.4, 100 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1X Protease Inhibitor Cocktail) without UV crosslink on ice for 15 min, followed by sonication and digestion with RNase T1 and Turbo DNAse at 37 C for 5 min. Digested lysates were cleared by centrifugation and immunoprecipitated using 100 μl of protein G Dynabeads pre-bound with 10 μg of anti-FLAG antibody at 4 C overnight. Samples were washed twice in high salt wash buffer (50 mM Tris-HCl pH 7.4, 1 M NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM EDTA), once in wash buffer (20 mM Tris-HCl pH 7.4, 0.2% Tween-20) and RNA was dephosporylated, treated with T4 PNK and ligated to 3' RNA adapter (/5Phos/rArGrArUrCrGrGrArArGrArGrCrArCrArCrGrUrC/3SpC3/) on beads. Samples (including SMinputs) were loaded on 8% SDS-PAGE gel for separation, transferred to a nitrocellulose membrane and RNA was isolated from membrane by cutting a region 75 kDa above NOP2/NSUN1. CLIP and SMinput libraries were prepared into paired-end high-throughput libraries as described by Van Norstrand et al (Methods Mol. Biol., 2017, 1648, 177-200.) and sent for sequencing on Illumina Hi-Seq 4000 platform (Novogene). Illumina HiSeq 4000 gds 305689051 GSM5689051 GEO Accession GSM5689051 SRX13128817 GSM5689052 SRP345880 SRS11062779 GSM5689052 RIP-Seq TRANSCRIPTOMIC other The eCLIP-sequencing protocol described by Van Nostrand et al (Methods Mol. Biol., 2017, 1648, 177-200.) was applied with minor modifications. Twenty million cells were lysed in CLIP buffer (50 mM Tris-HCl pH 7.4, 100 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1X Protease Inhibitor Cocktail) without UV crosslink on ice for 15 min, followed by sonication and digestion with RNase T1 and Turbo DNAse at 37 C for 5 min. Digested lysates were cleared by centrifugation and immunoprecipitated using 100 μl of protein G Dynabeads pre-bound with 10 μg of anti-FLAG antibody at 4 C overnight. Samples were washed twice in high salt wash buffer (50 mM Tris-HCl pH 7.4, 1 M NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM EDTA), once in wash buffer (20 mM Tris-HCl pH 7.4, 0.2% Tween-20) and RNA was dephosporylated, treated with T4 PNK and ligated to 3' RNA adapter (/5Phos/rArGrArUrCrGrGrArArGrArGrCrArCrArCrGrUrC/3SpC3/) on beads. Samples (including SMinputs) were loaded on 8% SDS-PAGE gel for separation, transferred to a nitrocellulose membrane and RNA was isolated from membrane by cutting a region 75 kDa above NOP2/NSUN1. CLIP and SMinput libraries were prepared into paired-end high-throughput libraries as described by Van Norstrand et al (Methods Mol. Biol., 2017, 1648, 177-200.) and sent for sequencing on Illumina Hi-Seq 4000 platform (Novogene). Illumina HiSeq 4000 gds 305689052 GSM5689052 GEO Accession GSM5689052 SRX13128818 GSM5689053 SRP345880 SRS11062777 GSM5689053 RIP-Seq TRANSCRIPTOMIC other The eCLIP-sequencing protocol described by Van Nostrand et al (Methods Mol. Biol., 2017, 1648, 177-200.) was applied with minor modifications. Twenty million cells were lysed in CLIP buffer (50 mM Tris-HCl pH 7.4, 100 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1X Protease Inhibitor Cocktail) without UV crosslink on ice for 15 min, followed by sonication and digestion with RNase T1 and Turbo DNAse at 37 C for 5 min. Digested lysates were cleared by centrifugation and immunoprecipitated using 100 μl of protein G Dynabeads pre-bound with 10 μg of anti-FLAG antibody at 4 C overnight. Samples were washed twice in high salt wash buffer (50 mM Tris-HCl pH 7.4, 1 M NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM EDTA), once in wash buffer (20 mM Tris-HCl pH 7.4, 0.2% Tween-20) and RNA was dephosporylated, treated with T4 PNK and ligated to 3' RNA adapter (/5Phos/rArGrArUrCrGrGrArArGrArGrCrArCrArCrGrUrC/3SpC3/) on beads. Samples (including SMinputs) were loaded on 8% SDS-PAGE gel for separation, transferred to a nitrocellulose membrane and RNA was isolated from membrane by cutting a region 75 kDa above NOP2/NSUN1. CLIP and SMinput libraries were prepared into paired-end high-throughput libraries as described by Van Norstrand et al (Methods Mol. Biol., 2017, 1648, 177-200.) and sent for sequencing on Illumina Hi-Seq 4000 platform (Novogene). Illumina HiSeq 4000 gds 305689053 GSM5689053 GEO Accession GSM5689053 SRX13128819 GSM5689054 SRP345880 SRS11062778 GSM5689054 RIP-Seq TRANSCRIPTOMIC other The eCLIP-sequencing protocol described by Van Nostrand et al (Methods Mol. Biol., 2017, 1648, 177-200.) was applied with minor modifications. Twenty million cells were lysed in CLIP buffer (50 mM Tris-HCl pH 7.4, 100 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1X Protease Inhibitor Cocktail) without UV crosslink on ice for 15 min, followed by sonication and digestion with RNase T1 and Turbo DNAse at 37 C for 5 min. Digested lysates were cleared by centrifugation and immunoprecipitated using 100 μl of protein G Dynabeads pre-bound with 10 μg of anti-FLAG antibody at 4 C overnight. Samples were washed twice in high salt wash buffer (50 mM Tris-HCl pH 7.4, 1 M NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM EDTA), once in wash buffer (20 mM Tris-HCl pH 7.4, 0.2% Tween-20) and RNA was dephosporylated, treated with T4 PNK and ligated to 3' RNA adapter (/5Phos/rArGrArUrCrGrGrArArGrArGrCrArCrArCrGrUrC/3SpC3/) on beads. Samples (including SMinputs) were loaded on 8% SDS-PAGE gel for separation, transferred to a nitrocellulose membrane and RNA was isolated from membrane by cutting a region 75 kDa above NOP2/NSUN1. CLIP and SMinput libraries were prepared into paired-end high-throughput libraries as described by Van Norstrand et al (Methods Mol. Biol., 2017, 1648, 177-200.) and sent for sequencing on Illumina Hi-Seq 4000 platform (Novogene). Illumina HiSeq 4000 gds 305689054 GSM5689054 GEO Accession GSM5689054 SRX13128820 GSM5689055 SRP345880 SRS11062780 GSM5689055 RIP-Seq TRANSCRIPTOMIC other The eCLIP-sequencing protocol described by Van Nostrand et al (Methods Mol. Biol., 2017, 1648, 177-200.) was applied with minor modifications. Twenty million cells were lysed in CLIP buffer (50 mM Tris-HCl pH 7.4, 100 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1X Protease Inhibitor Cocktail) without UV crosslink on ice for 15 min, followed by sonication and digestion with RNase T1 and Turbo DNAse at 37 C for 5 min. Digested lysates were cleared by centrifugation and immunoprecipitated using 100 μl of protein G Dynabeads pre-bound with 10 μg of anti-FLAG antibody at 4 C overnight. Samples were washed twice in high salt wash buffer (50 mM Tris-HCl pH 7.4, 1 M NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM EDTA), once in wash buffer (20 mM Tris-HCl pH 7.4, 0.2% Tween-20) and RNA was dephosporylated, treated with T4 PNK and ligated to 3' RNA adapter (/5Phos/rArGrArUrCrGrGrArArGrArGrCrArCrArCrGrUrC/3SpC3/) on beads. Samples (including SMinputs) were loaded on 8% SDS-PAGE gel for separation, transferred to a nitrocellulose membrane and RNA was isolated from membrane by cutting a region 75 kDa above NOP2/NSUN1. CLIP and SMinput libraries were prepared into paired-end high-throughput libraries as described by Van Norstrand et al (Methods Mol. Biol., 2017, 1648, 177-200.) and sent for sequencing on Illumina Hi-Seq 4000 platform (Novogene). Illumina HiSeq 4000 gds 305689055 GSM5689055 GEO Accession GSM5689055 SRX13128821 GSM5689056 SRP345880 SRS11062781 GSM5689056 RIP-Seq TRANSCRIPTOMIC other The eCLIP-sequencing protocol described by Van Nostrand et al (Methods Mol. Biol., 2017, 1648, 177-200.) was applied with minor modifications. Twenty million cells were lysed in CLIP buffer (50 mM Tris-HCl pH 7.4, 100 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1X Protease Inhibitor Cocktail) without UV crosslink on ice for 15 min, followed by sonication and digestion with RNase T1 and Turbo DNAse at 37 C for 5 min. Digested lysates were cleared by centrifugation and immunoprecipitated using 100 μl of protein G Dynabeads pre-bound with 10 μg of anti-FLAG antibody at 4 C overnight. Samples were washed twice in high salt wash buffer (50 mM Tris-HCl pH 7.4, 1 M NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM EDTA), once in wash buffer (20 mM Tris-HCl pH 7.4, 0.2% Tween-20) and RNA was dephosporylated, treated with T4 PNK and ligated to 3' RNA adapter (/5Phos/rArGrArUrCrGrGrArArGrArGrCrArCrArCrGrUrC/3SpC3/) on beads. Samples (including SMinputs) were loaded on 8% SDS-PAGE gel for separation, transferred to a nitrocellulose membrane and RNA was isolated from membrane by cutting a region 75 kDa above NOP2/NSUN1. CLIP and SMinput libraries were prepared into paired-end high-throughput libraries as described by Van Norstrand et al (Methods Mol. Biol., 2017, 1648, 177-200.) and sent for sequencing on Illumina Hi-Seq 4000 platform (Novogene). Illumina HiSeq 4000 gds 305689056 GSM5689056 GEO Accession GSM5689056 SRX13128822 GSM5689057 SRP345880 SRS11062782 GSM5689057 RIP-Seq TRANSCRIPTOMIC other The eCLIP-sequencing protocol described by Van Nostrand et al (Methods Mol. Biol., 2017, 1648, 177-200.) was applied with minor modifications. Twenty million cells were lysed in CLIP buffer (50 mM Tris-HCl pH 7.4, 100 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1X Protease Inhibitor Cocktail) without UV crosslink on ice for 15 min, followed by sonication and digestion with RNase T1 and Turbo DNAse at 37 C for 5 min. Digested lysates were cleared by centrifugation and immunoprecipitated using 100 μl of protein G Dynabeads pre-bound with 10 μg of anti-FLAG antibody at 4 C overnight. Samples were washed twice in high salt wash buffer (50 mM Tris-HCl pH 7.4, 1 M NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM EDTA), once in wash buffer (20 mM Tris-HCl pH 7.4, 0.2% Tween-20) and RNA was dephosporylated, treated with T4 PNK and ligated to 3' RNA adapter (/5Phos/rArGrArUrCrGrGrArArGrArGrCrArCrArCrGrUrC/3SpC3/) on beads. Samples (including SMinputs) were loaded on 8% SDS-PAGE gel for separation, transferred to a nitrocellulose membrane and RNA was isolated from membrane by cutting a region 75 kDa above NOP2/NSUN1. CLIP and SMinput libraries were prepared into paired-end high-throughput libraries as described by Van Norstrand et al (Methods Mol. Biol., 2017, 1648, 177-200.) and sent for sequencing on Illumina Hi-Seq 4000 platform (Novogene). Illumina HiSeq 4000 gds 305689057 GSM5689057 GEO Accession GSM5689057 SRX13128823 GSM5689058 SRP345880 SRS11062783 GSM5689058 RIP-Seq TRANSCRIPTOMIC other The eCLIP-sequencing protocol described by Van Nostrand et al (Methods Mol. Biol., 2017, 1648, 177-200.) was applied with minor modifications. Twenty million cells were lysed in CLIP buffer (50 mM Tris-HCl pH 7.4, 100 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1X Protease Inhibitor Cocktail) without UV crosslink on ice for 15 min, followed by sonication and digestion with RNase T1 and Turbo DNAse at 37 C for 5 min. Digested lysates were cleared by centrifugation and immunoprecipitated using 100 μl of protein G Dynabeads pre-bound with 10 μg of anti-FLAG antibody at 4 C overnight. Samples were washed twice in high salt wash buffer (50 mM Tris-HCl pH 7.4, 1 M NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM EDTA), once in wash buffer (20 mM Tris-HCl pH 7.4, 0.2% Tween-20) and RNA was dephosporylated, treated with T4 PNK and ligated to 3' RNA adapter (/5Phos/rArGrArUrCrGrGrArArGrArGrCrArCrArCrGrUrC/3SpC3/) on beads. Samples (including SMinputs) were loaded on 8% SDS-PAGE gel for separation, transferred to a nitrocellulose membrane and RNA was isolated from membrane by cutting a region 75 kDa above NOP2/NSUN1. CLIP and SMinput libraries were prepared into paired-end high-throughput libraries as described by Van Norstrand et al (Methods Mol. Biol., 2017, 1648, 177-200.) and sent for sequencing on Illumina Hi-Seq 4000 platform (Novogene). Illumina HiSeq 4000 gds 305689058 GSM5689058 GEO Accession GSM5689058