SRS11586224 SAMN24811772 SARS-CoV-2: wastewater surveillance 527639 wastewater metagenome PAS grab 11:05am bioproject 757447 collection_date 2021-12-14 collection_site_id PAS ww_population 36969 ww_sample_duration 0 ww_sample_matrix raw wastewater ww_sample_type grab collection_volume 4L ww_surv_target_1 SARS-CoV-2 ww_surv_target_1_known_present no purpose_of_ww_sampling public health surveillance community-level ww_flow not collected ww_industrial_effluent_percent 12.4 ww_ph 6.61 ww_population_source EPA Echo DMR ww_pre_treatment Bar screen (2") ww_sample_site wastewater treatment plant ww_temperature 22.5 ww_total_suspended_solids 134 ww_surv_target_1_gene N (N1) ww_surv_target_1_protocol RT-qPCR Detection of SARS-CoV-2 from Wastewater Using the AB 7500 (protocols.io) ww_surv_target_2 SARS-CoV-2 ww_surv_target_2_conc 30 ww_surv_target_2_conc_unit Ct values ww_surv_target_2_gene N (N2) ww_surv_target_2_known_present yes ww_surv_target_2_protocol RT-qPCR Detection of SARS-CoV-2 from Wastewater Using the AB 7500 (protocols.io) concentration_method peg precipitation + ultracentrifugation extraction_control Murine norovirus extraction_method qiagen rneasy kit ww_surv_target_1_conc <15 ww_surv_target_1_conc_unit Ct values Collection_time 5:21:00 PM quality_control_method CFSAN Wastewater Analysis Pipeline (C-WAP) quality_control_determination sequence flagged for potential quality control issues quality_control_details low % genome captured quality_control_issues low % genome captured collected by FDA Center for Food Safety and Applied Nutrition isolation source wastewater sequenced by FDA Center for Food Safety and Applied Nutrition geo loc name USA: Mississippi Collection_time 0.7229166666666668 sample_processing_details not collected sample_processing_id not collected sample_processing not collected ww_endogenous_control_1_concentration not collected ww_endogenous_control_1_protocol RT-qPCR Detection of Process Controls (Murine noroviurs and crAssphage) from Wastewater using AB 7500 (protocols.io) ww_endogenous_control_1 crAssphage ww_endogenous_control_2_conc not collected ww_endogenous_control_2_protocol not collected ww_endogenous_control_2 not collected ww_endogenous_control_2_units not collected ww_primary_sludge_retention_time not collected ww_processing_protocol not collected ww_sample_salinity 600 ww_surveillance_system_sample_id not collected instantaneous_flow 14384564.8 BioSampleModel SARS-CoV-2: wastewater surveillance SRS11586225 SAMN24811773 SARS-CoV-2: wastewater surveillance 527639 wastewater metagenome MOB grab 9:55am bioproject 757447 collection_date 2021-12-14 collection_site_id MOB ww_population 125030 ww_sample_duration 0 ww_sample_matrix raw wastewater ww_sample_type grab collection_volume 4L ww_surv_target_1 SARS-CoV-2 ww_surv_target_1_known_present no purpose_of_ww_sampling public health surveillance community-level ww_flow not collected ww_industrial_effluent_percent 8 ww_ph 6.88 ww_population_source EPA Echo DMR ww_pre_treatment Bar screen (2") ww_sample_site wastewater treatment plant ww_temperature 22.5 ww_total_suspended_solids 132 ww_surv_target_1_gene N (N1) ww_surv_target_1_protocol RT-qPCR Detection of SARS-CoV-2 from Wastewater Using the AB 7500 (protocols.io) ww_surv_target_2 SARS-CoV-2 ww_surv_target_2_conc 33 ww_surv_target_2_conc_unit Ct values ww_surv_target_2_gene N (N2) ww_surv_target_2_known_present yes ww_surv_target_2_protocol RT-qPCR Detection of SARS-CoV-2 from Wastewater Using the AB 7500 (protocols.io) concentration_method peg precipitation + ultracentrifugation extraction_control Murine norovirus extraction_method qiagen rneasy kit ww_surv_target_1_conc 16 ww_surv_target_1_conc_unit Ct values Collection_time 4:10:00 PM collected by FDA Center for Food Safety and Applied Nutrition isolation source wastewater sequenced by FDA Center for Food Safety and Applied Nutrition geo loc name USA: Alabama Collection_time 0.6736111111111112 sample_processing_details not collected sample_processing_id not collected sample_processing not collected ww_endogenous_control_1_concentration not collected ww_endogenous_control_1_protocol RT-qPCR Detection of Process Controls (Murine noroviurs and crAssphage) from Wastewater using AB 7500 (protocols.io) ww_endogenous_control_1 crAssphage ww_endogenous_control_2_conc not collected ww_endogenous_control_2_protocol not collected ww_endogenous_control_2 not collected ww_endogenous_control_2_units not collected ww_primary_sludge_retention_time not collected ww_processing_protocol not collected ww_sample_salinity 700 ww_surveillance_system_sample_id not collected instantaneous_flow 99177789.2 BioSampleModel SARS-CoV-2: wastewater surveillance SRS11586226 SAMN24811774 SARS-CoV-2: wastewater surveillance 527639 wastewater metagenome PAS grab 11:05am bioproject 757447 collection_date 2021-12-21 collection_site_id PAS ww_population 36969 ww_sample_duration 0 ww_sample_matrix raw wastewater ww_sample_type grab collection_volume 4L ww_surv_target_1 SARS-CoV-2 ww_surv_target_1_known_present yes purpose_of_ww_sampling public health surveillance community-level ww_flow not collected ww_industrial_effluent_percent 12.4 ww_ph 6.48 ww_population_source EPA Echo DMR ww_pre_treatment Bar screen (2") ww_sample_site wastewater treatment plant ww_temperature 21.8 ww_total_suspended_solids 136 ww_surv_target_1_gene N (N1) ww_surv_target_1_protocol RT-qPCR Detection of SARS-CoV-2 from Wastewater Using the AB 7500 (protocols.io) ww_surv_target_2 SARS-CoV-2 ww_surv_target_2_conc 32 ww_surv_target_2_conc_unit Ct values ww_surv_target_2_gene N (N2) ww_surv_target_2_known_present yes ww_surv_target_2_protocol RT-qPCR Detection of SARS-CoV-2 from Wastewater Using the AB 7500 (protocols.io) concentration_method peg precipitation + ultracentrifugation extraction_control Murine norovirus extraction_method qiagen rneasy kit ww_surv_target_1_conc 16 ww_surv_target_1_conc_unit Ct values Collection_time 4:57:00 PM collected by FDA Center for Food Safety and Applied Nutrition isolation source wastewater sequenced by FDA Center for Food Safety and Applied Nutrition geo loc name USA: Mississippi Collection_time 0.7062499999999999 sample_processing_details not collected sample_processing_id not collected sample_processing not collected ww_endogenous_control_1_concentration not collected ww_endogenous_control_1_protocol RT-qPCR Detection of Process Controls (Murine noroviurs and crAssphage) from Wastewater using AB 7500 (protocols.io) ww_endogenous_control_1 crAssphage ww_endogenous_control_2_conc not collected ww_endogenous_control_2_protocol not collected ww_endogenous_control_2 not collected ww_endogenous_control_2_units not collected ww_primary_sludge_retention_time not collected ww_processing_protocol not collected ww_sample_salinity 1200 ww_surveillance_system_sample_id not collected instantaneous_flow 8218129 BioSampleModel SARS-CoV-2: wastewater surveillance SRS11586227 SAMN24811775 SARS-CoV-2: wastewater surveillance 527639 wastewater metagenome MOB grab 9:55am bioproject 757447 collection_date 2021-12-21 collection_site_id MOB ww_population 125030 ww_sample_duration 0 ww_sample_matrix raw wastewater ww_sample_type grab collection_volume 4L ww_surv_target_1 SARS-CoV-2 ww_surv_target_1_known_present yes purpose_of_ww_sampling public health surveillance community-level ww_flow not collected ww_industrial_effluent_percent 8 ww_ph 6.89 ww_population_source EPA Echo DMR ww_pre_treatment Bar screen (2") ww_sample_site wastewater treatment plant ww_temperature 21.6 ww_total_suspended_solids 154 ww_surv_target_1_gene N (N1) ww_surv_target_1_protocol RT-qPCR Detection of SARS-CoV-2 from Wastewater Using the AB 7500 (protocols.io) ww_surv_target_2 SARS-CoV-2 ww_surv_target_2_conc 260 ww_surv_target_2_conc_unit Ct values ww_surv_target_2_gene N (N2) ww_surv_target_2_known_present no ww_surv_target_2_protocol RT-qPCR Detection of SARS-CoV-2 from Wastewater Using the AB 7500 (protocols.io) concentration_method peg precipitation + ultracentrifugation extraction_control Murine norovirus extraction_method qiagen rneasy kit ww_surv_target_1_conc 110 ww_surv_target_1_conc_unit Ct values Collection_time 3:31:00 PM collected by FDA Center for Food Safety and Applied Nutrition isolation source wastewater sequenced by FDA Center for Food Safety and Applied Nutrition geo loc name USA: Alabama Collection_time 0.6465277777777778 sample_processing_details not collected sample_processing_id not collected sample_processing not collected ww_endogenous_control_1_concentration not collected ww_endogenous_control_1_protocol RT-qPCR Detection of Process Controls (Murine noroviurs and crAssphage) from Wastewater using AB 7500 (protocols.io) ww_endogenous_control_1 crAssphage ww_endogenous_control_2_conc not collected ww_endogenous_control_2_protocol not collected ww_endogenous_control_2 not collected ww_endogenous_control_2_units not collected ww_primary_sludge_retention_time not collected ww_processing_protocol not collected ww_sample_salinity 600 ww_surveillance_system_sample_id not collected instantaneous_flow 69878701.8 BioSampleModel SARS-CoV-2: wastewater surveillance SRS11586228 SAMN24811776 SARS-CoV-2: wastewater surveillance 527639 wastewater metagenome PAS grab 11:05am bioproject 757447 collection_date 2021-12-28 collection_site_id PAS ww_population 36969 ww_sample_duration 0 ww_sample_matrix raw wastewater ww_sample_type grab collection_volume 4L ww_surv_target_1 SARS-CoV-2 ww_surv_target_1_known_present yes purpose_of_ww_sampling public health surveillance community-level ww_flow not collected ww_industrial_effluent_percent 12.4 ww_ph 7 ww_population_source EPA Echo DMR ww_pre_treatment Bar screen (2") ww_sample_site wastewater treatment plant ww_temperature 22.1 ww_total_suspended_solids 144 ww_surv_target_1_gene N (N1) ww_surv_target_1_protocol RT-qPCR Detection of SARS-CoV-2 from Wastewater Using the AB 7500 (protocols.io) ww_surv_target_2 SARS-CoV-2 ww_surv_target_2_conc 340 ww_surv_target_2_conc_unit Ct values ww_surv_target_2_gene N (N2) ww_surv_target_2_known_present yes ww_surv_target_2_protocol RT-qPCR Detection of SARS-CoV-2 from Wastewater Using the AB 7500 (protocols.io) concentration_method peg precipitation + ultracentrifugation extraction_control Murine norovirus extraction_method qiagen rneasy kit ww_surv_target_1_conc 200 ww_surv_target_1_conc_unit Ct values Collection_time 4:52:00 PM collected by FDA Center for Food Safety and Applied Nutrition isolation source wastewater sequenced by FDA Center for Food Safety and Applied Nutrition geo loc name USA: Mississippi Collection_time 0.7027777777777778 sample_processing_details not collected sample_processing_id not collected sample_processing not collected ww_endogenous_control_1_concentration not collected ww_endogenous_control_1_protocol RT-qPCR Detection of Process Controls (Murine noroviurs and crAssphage) from Wastewater using AB 7500 (protocols.io) ww_endogenous_control_1 crAssphage ww_endogenous_control_2_conc not collected ww_endogenous_control_2_protocol not collected ww_endogenous_control_2 not collected ww_endogenous_control_2_units not collected ww_primary_sludge_retention_time not collected ww_processing_protocol not collected ww_sample_salinity 500 ww_surveillance_system_sample_id not collected instantaneous_flow 10712715.4 BioSampleModel SARS-CoV-2: wastewater surveillance SRS11586229 SAMN24811777 SARS-CoV-2: wastewater surveillance 527639 wastewater metagenome MOB grab 9:55am bioproject 757447 collection_date 2021-12-28 collection_site_id MOB ww_population 125030 ww_sample_duration 0 ww_sample_matrix raw wastewater ww_sample_type grab collection_volume 4L ww_surv_target_1 SARS-CoV-2 ww_surv_target_1_known_present yes purpose_of_ww_sampling public health surveillance community-level ww_flow not collected ww_industrial_effluent_percent 8 ww_ph 7 ww_population_source EPA Echo DMR ww_pre_treatment Bar screen (2") ww_sample_site wastewater treatment plant ww_temperature 18.1 ww_total_suspended_solids 182 ww_surv_target_1_gene N (N1) ww_surv_target_1_protocol RT-qPCR Detection of SARS-CoV-2 from Wastewater Using the AB 7500 (protocols.io) ww_surv_target_2 SARS-CoV-2 ww_surv_target_2_conc 370 ww_surv_target_2_conc_unit Ct values ww_surv_target_2_gene N (N2) ww_surv_target_2_known_present yes ww_surv_target_2_protocol RT-qPCR Detection of SARS-CoV-2 from Wastewater Using the AB 7500 (protocols.io) concentration_method peg precipitation + ultracentrifugation extraction_control Murine norovirus extraction_method qiagen rneasy kit ww_surv_target_1_conc 190 ww_surv_target_1_conc_unit Ct values Collection_time 3:23:00 PM quality_control_method CFSAN Wastewater Analysis Pipeline (C-WAP) quality_control_determination sequence flagged for potential quality control issues quality_control_details low % genome captured quality_control_issues low % genome captured collected by FDA Center for Food Safety and Applied Nutrition isolation source wastewater sequenced by FDA Center for Food Safety and Applied Nutrition geo loc name USA: Alabama Collection_time 0.6409722222222222 sample_processing_details not collected sample_processing_id not collected sample_processing not collected ww_endogenous_control_1_concentration not collected ww_endogenous_control_1_protocol RT-qPCR Detection of Process Controls (Murine noroviurs and crAssphage) from Wastewater using AB 7500 (protocols.io) ww_endogenous_control_1 crAssphage ww_endogenous_control_2_conc not collected ww_endogenous_control_2_protocol not collected ww_endogenous_control_2 not collected ww_endogenous_control_2_units not collected ww_primary_sludge_retention_time not collected ww_processing_protocol not collected ww_sample_salinity 900 ww_surveillance_system_sample_id not collected instantaneous_flow 58787445.3 BioSampleModel SARS-CoV-2: wastewater surveillance SRS11586230 SAMN24811778 SARS-CoV-2: wastewater surveillance 527639 wastewater metagenome PAS grab 11:05am bioproject 757447 collection_date 2022-01-04 collection_site_id PAS ww_population 36969 ww_sample_duration 0 ww_sample_matrix raw wastewater ww_sample_type grab collection_volume 4L ww_surv_target_1 SARS-CoV-2 ww_surv_target_1_known_present yes purpose_of_ww_sampling public health surveillance community-level ww_flow not collected ww_industrial_effluent_percent 12.4 ww_ph 6.23 ww_population_source EPA Echo DMR ww_pre_treatment Bar screen (2") ww_sample_site wastewater treatment plant ww_temperature 21.3 ww_total_suspended_solids 190 ww_surv_target_1_gene N (N1) ww_surv_target_1_protocol RT-qPCR Detection of SARS-CoV-2 from Wastewater Using the AB 7500 (protocols.io) ww_surv_target_2 SARS-CoV-2 ww_surv_target_2_conc 6900 ww_surv_target_2_conc_unit Ct values ww_surv_target_2_gene N (N2) ww_surv_target_2_known_present yes ww_surv_target_2_protocol RT-qPCR Detection of SARS-CoV-2 from Wastewater Using the AB 7500 (protocols.io) concentration_method peg precipitation + ultracentrifugation extraction_control Murine norovirus extraction_method qiagen rneasy kit ww_surv_target_1_conc 3100 ww_surv_target_1_conc_unit Ct values Collection_time 5:11:00 PM collected by FDA Center for Food Safety and Applied Nutrition isolation source wastewater sequenced by FDA Center for Food Safety and Applied Nutrition geo loc name USA: Mississippi Collection_time 0.7159722222222222 sample_processing_details not collected sample_processing_id not collected sample_processing not collected ww_endogenous_control_1_concentration not collected ww_endogenous_control_1_protocol RT-qPCR Detection of Process Controls (Murine noroviurs and crAssphage) from Wastewater using AB 7500 (protocols.io) ww_endogenous_control_1 crAssphage ww_endogenous_control_2_conc not collected ww_endogenous_control_2_protocol not collected ww_endogenous_control_2 not collected ww_endogenous_control_2_units not collected ww_primary_sludge_retention_time not collected ww_processing_protocol not collected ww_sample_salinity 500 ww_surveillance_system_sample_id not collected instantaneous_flow 11886193.1 BioSampleModel SARS-CoV-2: wastewater surveillance SRS11586231 SAMN24811779 SARS-CoV-2: wastewater surveillance 527639 wastewater metagenome MOB grab 9:55am bioproject 757447 collection_date 2022-01-04 collection_site_id MOB ww_population 125030 ww_sample_duration 0 ww_sample_matrix raw wastewater ww_sample_type grab collection_volume 4L ww_surv_target_1 SARS-CoV-2 ww_surv_target_1_known_present yes purpose_of_ww_sampling public health surveillance community-level ww_flow not collected ww_industrial_effluent_percent 8 ww_ph 5.41 ww_population_source EPA Echo DMR ww_pre_treatment Bar screen (2") ww_sample_site wastewater treatment plant ww_temperature 21.5 ww_total_suspended_solids 130 ww_surv_target_1_gene N (N1) ww_surv_target_1_protocol RT-qPCR Detection of SARS-CoV-2 from Wastewater Using the AB 7500 (protocols.io) ww_surv_target_2 SARS-CoV-2 ww_surv_target_2_conc 9800 ww_surv_target_2_conc_unit Ct values ww_surv_target_2_gene N (N2) ww_surv_target_2_known_present yes ww_surv_target_2_protocol RT-qPCR Detection of SARS-CoV-2 from Wastewater Using the AB 7500 (protocols.io) concentration_method peg precipitation + ultracentrifugation extraction_control Murine norovirus extraction_method qiagen rneasy kit ww_surv_target_1_conc 4500 ww_surv_target_1_conc_unit Ct values Collection_time 4:07:00 PM collected by FDA Center for Food Safety and Applied Nutrition isolation source wastewater sequenced by FDA Center for Food Safety and Applied Nutrition geo loc name USA: Alabama Collection_time 0.6715277777777778 sample_processing_details not collected sample_processing_id not collected sample_processing not collected ww_endogenous_control_1_concentration not collected ww_endogenous_control_1_protocol RT-qPCR Detection of Process Controls (Murine noroviurs and crAssphage) from Wastewater using AB 7500 (protocols.io) ww_endogenous_control_1 crAssphage ww_endogenous_control_2_conc not collected ww_endogenous_control_2_protocol not collected ww_endogenous_control_2 not collected ww_endogenous_control_2_units not collected ww_primary_sludge_retention_time not collected ww_processing_protocol not collected ww_sample_salinity 500 ww_surveillance_system_sample_id not collected instantaneous_flow 61550795.9 BioSampleModel SARS-CoV-2: wastewater surveillance