SRX13805545 Int_benomyl-10_14d SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686930 Int_benomyl-10_14d Int_benomyl-10_14d AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805546 Int_benomyl-1_14d_r1 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686931 Int_benomyl-1_14d_r1 Int_benomyl-1_14d_r1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805547 Int_imidacloprid-2_14d_r2 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686932 Int_imidacloprid-2_14d_r2 Int_imidacloprid-2_14d_r2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805548 Int_metribuzin-10_14d_r1 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686933 Int_metribuzin-10_14d_r1 Int_metribuzin-10_14d_r1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805549 Int_metribuzin-10_14d_r2 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686934 Int_metribuzin-10_14d_r2 Int_metribuzin-10_14d_r2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805550 Int_metribuzin-1_14d_r1 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686935 Int_metribuzin-1_14d_r1 Int_metribuzin-1_14d_r1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805551 Int_metribuzin-1_14d_r2 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686936 Int_metribuzin-1_14d_r2 Int_metribuzin-1_14d_r2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805552 Int_metribuzin-2_14d_r1 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686937 Int_metribuzin-2_14d_r1 Int_metribuzin-2_14d_r1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805553 Int_metribuzin-2_14d_r2 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686938 Int_metribuzin-2_14d_r2 Int_metribuzin-2_14d_r2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805554 Int_mix-10_14d_r1 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686939 Int_mix-10_14d_r1 Int_mix-10_14d_r1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805555 Int_mix-10_14d_r2 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686940 Int_mix-10_14d_r2 Int_mix-10_14d_r2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805556 Int_mix-10_7d_r1 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686941 Int_mix-10_7d_r1 Int_mix-10_7d_r1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805557 Int_benomyl-1_14d_r2 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686942 Int_benomyl-1_14d_r2 Int_benomyl-1_14d_r2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805558 Int_mix-10_7d_r2 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686943 Int_mix-10_7d_r2 Int_mix-10_7d_r2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805559 Int_mix-1_14d_r1 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686944 Int_mix-1_14d_r1 Int_mix-1_14d_r1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805560 Int_mix-1_14d_r2 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686945 Int_mix-1_14d_r2 Int_mix-1_14d_r2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805561 Int_mix-control_7d_r2 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686946 Int_mix-control_7d_r2 Int_mix-control_7d_r2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805562 Int_mix-control_14d_r1 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686947 Int_mix-control_14d_r1 Int_mix-control_14d_r1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805563 Int_mix-control_14d_r2 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686948 Int_mix-control_14d_r2 Int_mix-control_14d_r2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805564 Int_single-control_14d_r1 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686949 Int_single-control_14d_r1 Int_single-control_14d_r1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805565 Int_single-control_14d_r2 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686950 Int_single-control_14d_r2 Int_single-control_14d_r2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805566 Int_benomyl-2_14d_r2 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686951 Int_benomyl-2_14d_r2 Int_benomyl-2_14d_r2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805567 Int_imidacloprid-10_14d_r1 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686952 Int_imidacloprid-10_14d_r1 Int_imidacloprid-10_14d_r1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805568 Int_imidacloprid-10_14d_r2 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686953 Int_imidacloprid-10_14d_r2 Int_imidacloprid-10_14d_r2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805569 Int_mix-1_7d_r1 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686954 Int_mix-1_7d_r1 Int_mix-1_7d_r1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805570 Int_mix-1_7d_r2 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686955 Int_mix-1_7d_r2 Int_mix-1_7d_r2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805571 Int_mix-2_14d_r1 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686956 Int_mix-2_14d_r1 Int_mix-2_14d_r1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805572 Int_mix-2_14d_r2 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686957 Int_mix-2_14d_r2 Int_mix-2_14d_r2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805573 Int_mix-2_7d_r1 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686958 Int_mix-2_7d_r1 Int_mix-2_7d_r1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805574 Int_mix-2_7d_r2 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686959 Int_mix-2_7d_r2 Int_mix-2_7d_r2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805575 Int_mix-control_7d_r1 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686960 Int_mix-control_7d_r1 Int_mix-control_7d_r1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805576 Int_benomyl-2_14d_r1 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686961 Int_benomyl-2_14d_r1 Int_benomyl-2_14d_r1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805577 Int_imidacloprid-1_14d_r1 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686962 Int_imidacloprid-1_14d_r1 Int_imidacloprid-1_14d_r1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805578 Int_imidacloprid-1_14d_r2 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686963 Int_imidacloprid-1_14d_r2 Int_imidacloprid-1_14d_r2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX13805579 Int_imidacloprid-2_14d_r1 SRP355289 bp0 The extraction of total DNA from a 0.5 mg sample of the intestines of earthworms was carried out using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturer's protocol. The isolated DNA extracts were stored at -20 C. The samples of isolated DNA were diluted 500-fold. Amplification of the V4 variable region of the 16S rRNA gene was carried out in 1 round using forward and reverse primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with two-index multiplexing of the samples (Fadrosh et al., 2014). These primers are specific both to bacteria and to archaea. The PCR products were purified using the Cleanup Mini kit (Evrogen, Russia) for DNA isolation from reaction mixtures. The concentration of the obtained 16S rRNA libraries in solution was measured on a Qubit fluorometer (Invitrogen, USA) using the Quant-iT dsDNA High-Sensitivity Assay Kit. The purified amplicons were mixed equimolarly in accordance with the concentration measurements. The quality of the resulting library prepared for sequencing was assessed by agarose gel electrophoresis. Further sample preparation and sequencing of the pooled sample was carried out using the MiSeq Reagent Kit v2 (500 cycles) and an MiSeq sequencer (Illumina, USA). SRS11686964 Int_imidacloprid-2_14d_r1 Int_imidacloprid-2_14d_r1 AMPLICON METAGENOMIC PCR Illumina MiSeq