SRX13844945 GSM5827689_r1 SRP356002 PRJNA799027 SRS11722063 GSM5827689 GSM5827689 RNA-Seq TRANSCRIPTOMIC cDNA Either total RNA was extracted directly with Trizol containing a capped RNA spike-in molecule, or cells were lysed on plate in 20 mM Tris pH 7.4; 150 mM NaCl; 5 mM MgCl2; 1% Triton X-100; 1 mM DTT; 100 ug/ml cycloheximide. Clarified lysates were then separated into a subpolysomal and polysomal fraction on a 5-50% sucrose gradient, and an equal amount of the capped spike-in RNA described above was added to each fraction. Total RNA was retrieved from both fractions by proteinase K treatment followed by acid phenol extraction. The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3' end of the cDNA was then ligated to a double stranded DNA linker with a 3' NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3' adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of Illumina sequences in a second PCR reaction. Illumina NovaSeq 6000 SRX13844946 GSM5827690_r1 SRP356002 PRJNA799027 SRS11722064 GSM5827690 GSM5827690 RNA-Seq TRANSCRIPTOMIC cDNA Either total RNA was extracted directly with Trizol containing a capped RNA spike-in molecule, or cells were lysed on plate in 20 mM Tris pH 7.4; 150 mM NaCl; 5 mM MgCl2; 1% Triton X-100; 1 mM DTT; 100 ug/ml cycloheximide. Clarified lysates were then separated into a subpolysomal and polysomal fraction on a 5-50% sucrose gradient, and an equal amount of the capped spike-in RNA described above was added to each fraction. Total RNA was retrieved from both fractions by proteinase K treatment followed by acid phenol extraction. The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3' end of the cDNA was then ligated to a double stranded DNA linker with a 3' NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3' adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of Illumina sequences in a second PCR reaction. Illumina NovaSeq 6000 SRX13844947 GSM5827691_r1 SRP356002 PRJNA799027 SRS11722065 GSM5827691 GSM5827691 RNA-Seq TRANSCRIPTOMIC cDNA Either total RNA was extracted directly with Trizol containing a capped RNA spike-in molecule, or cells were lysed on plate in 20 mM Tris pH 7.4; 150 mM NaCl; 5 mM MgCl2; 1% Triton X-100; 1 mM DTT; 100 ug/ml cycloheximide. Clarified lysates were then separated into a subpolysomal and polysomal fraction on a 5-50% sucrose gradient, and an equal amount of the capped spike-in RNA described above was added to each fraction. Total RNA was retrieved from both fractions by proteinase K treatment followed by acid phenol extraction. The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3' end of the cDNA was then ligated to a double stranded DNA linker with a 3' NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3' adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of Illumina sequences in a second PCR reaction. Illumina NovaSeq 6000 SRX13844948 GSM5827692_r1 SRP356002 PRJNA799027 SRS11722066 GSM5827692 GSM5827692 RNA-Seq TRANSCRIPTOMIC cDNA Either total RNA was extracted directly with Trizol containing a capped RNA spike-in molecule, or cells were lysed on plate in 20 mM Tris pH 7.4; 150 mM NaCl; 5 mM MgCl2; 1% Triton X-100; 1 mM DTT; 100 ug/ml cycloheximide. Clarified lysates were then separated into a subpolysomal and polysomal fraction on a 5-50% sucrose gradient, and an equal amount of the capped spike-in RNA described above was added to each fraction. Total RNA was retrieved from both fractions by proteinase K treatment followed by acid phenol extraction. The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3' end of the cDNA was then ligated to a double stranded DNA linker with a 3' NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3' adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of Illumina sequences in a second PCR reaction. Illumina NovaSeq 6000 SRX13844949 GSM5827693_r1 SRP356002 PRJNA799027 SRS11722067 GSM5827693 GSM5827693 RNA-Seq TRANSCRIPTOMIC cDNA Either total RNA was extracted directly with Trizol containing a capped RNA spike-in molecule, or cells were lysed on plate in 20 mM Tris pH 7.4; 150 mM NaCl; 5 mM MgCl2; 1% Triton X-100; 1 mM DTT; 100 ug/ml cycloheximide. Clarified lysates were then separated into a subpolysomal and polysomal fraction on a 5-50% sucrose gradient, and an equal amount of the capped spike-in RNA described above was added to each fraction. Total RNA was retrieved from both fractions by proteinase K treatment followed by acid phenol extraction. The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3' end of the cDNA was then ligated to a double stranded DNA linker with a 3' NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3' adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of Illumina sequences in a second PCR reaction. Illumina NovaSeq 6000 SRX13844950 GSM5827694_r1 SRP356002 PRJNA799027 SRS11722068 GSM5827694 GSM5827694 RNA-Seq TRANSCRIPTOMIC cDNA Either total RNA was extracted directly with Trizol containing a capped RNA spike-in molecule, or cells were lysed on plate in 20 mM Tris pH 7.4; 150 mM NaCl; 5 mM MgCl2; 1% Triton X-100; 1 mM DTT; 100 ug/ml cycloheximide. Clarified lysates were then separated into a subpolysomal and polysomal fraction on a 5-50% sucrose gradient, and an equal amount of the capped spike-in RNA described above was added to each fraction. Total RNA was retrieved from both fractions by proteinase K treatment followed by acid phenol extraction. The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3' end of the cDNA was then ligated to a double stranded DNA linker with a 3' NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3' adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of Illumina sequences in a second PCR reaction. Illumina NovaSeq 6000 SRX13844951 GSM5827695_r1 SRP356002 PRJNA799027 SRS11722069 GSM5827695 GSM5827695 RNA-Seq TRANSCRIPTOMIC cDNA Either total RNA was extracted directly with Trizol containing a capped RNA spike-in molecule, or cells were lysed on plate in 20 mM Tris pH 7.4; 150 mM NaCl; 5 mM MgCl2; 1% Triton X-100; 1 mM DTT; 100 ug/ml cycloheximide. Clarified lysates were then separated into a subpolysomal and polysomal fraction on a 5-50% sucrose gradient, and an equal amount of the capped spike-in RNA described above was added to each fraction. Total RNA was retrieved from both fractions by proteinase K treatment followed by acid phenol extraction. The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3' end of the cDNA was then ligated to a double stranded DNA linker with a 3' NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3' adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of Illumina sequences in a second PCR reaction. Illumina NovaSeq 6000 SRX13844952 GSM5827696_r1 SRP356002 PRJNA799027 SRS11722070 GSM5827696 GSM5827696 RNA-Seq TRANSCRIPTOMIC cDNA Either total RNA was extracted directly with Trizol containing a capped RNA spike-in molecule, or cells were lysed on plate in 20 mM Tris pH 7.4; 150 mM NaCl; 5 mM MgCl2; 1% Triton X-100; 1 mM DTT; 100 ug/ml cycloheximide. Clarified lysates were then separated into a subpolysomal and polysomal fraction on a 5-50% sucrose gradient, and an equal amount of the capped spike-in RNA described above was added to each fraction. Total RNA was retrieved from both fractions by proteinase K treatment followed by acid phenol extraction. The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3' end of the cDNA was then ligated to a double stranded DNA linker with a 3' NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3' adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of Illumina sequences in a second PCR reaction. Illumina NovaSeq 6000 SRX13844953 GSM5827697_r1 SRP356002 PRJNA799027 SRS11722071 GSM5827697 GSM5827697 RNA-Seq TRANSCRIPTOMIC cDNA Either total RNA was extracted directly with Trizol containing a capped RNA spike-in molecule, or cells were lysed on plate in 20 mM Tris pH 7.4; 150 mM NaCl; 5 mM MgCl2; 1% Triton X-100; 1 mM DTT; 100 ug/ml cycloheximide. Clarified lysates were then separated into a subpolysomal and polysomal fraction on a 5-50% sucrose gradient, and an equal amount of the capped spike-in RNA described above was added to each fraction. Total RNA was retrieved from both fractions by proteinase K treatment followed by acid phenol extraction. The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3' end of the cDNA was then ligated to a double stranded DNA linker with a 3' NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3' adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of Illumina sequences in a second PCR reaction. Illumina NovaSeq 6000 SRX13844954 GSM5827698_r1 SRP356002 PRJNA799027 SRS11722072 GSM5827698 GSM5827698 RNA-Seq TRANSCRIPTOMIC cDNA Either total RNA was extracted directly with Trizol containing a capped RNA spike-in molecule, or cells were lysed on plate in 20 mM Tris pH 7.4; 150 mM NaCl; 5 mM MgCl2; 1% Triton X-100; 1 mM DTT; 100 ug/ml cycloheximide. Clarified lysates were then separated into a subpolysomal and polysomal fraction on a 5-50% sucrose gradient, and an equal amount of the capped spike-in RNA described above was added to each fraction. Total RNA was retrieved from both fractions by proteinase K treatment followed by acid phenol extraction. The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3' end of the cDNA was then ligated to a double stranded DNA linker with a 3' NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3' adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of Illumina sequences in a second PCR reaction. Illumina NovaSeq 6000 SRX13844955 GSM5827699_r1 SRP356002 PRJNA799027 SRS11722073 GSM5827699 GSM5827699 RNA-Seq TRANSCRIPTOMIC cDNA Either total RNA was extracted directly with Trizol containing a capped RNA spike-in molecule, or cells were lysed on plate in 20 mM Tris pH 7.4; 150 mM NaCl; 5 mM MgCl2; 1% Triton X-100; 1 mM DTT; 100 ug/ml cycloheximide. Clarified lysates were then separated into a subpolysomal and polysomal fraction on a 5-50% sucrose gradient, and an equal amount of the capped spike-in RNA described above was added to each fraction. Total RNA was retrieved from both fractions by proteinase K treatment followed by acid phenol extraction. The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3' end of the cDNA was then ligated to a double stranded DNA linker with a 3' NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3' adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of Illumina sequences in a second PCR reaction. Illumina NovaSeq 6000 SRX13844956 GSM5827700_r1 SRP356002 PRJNA799027 SRS11722074 GSM5827700 GSM5827700 RNA-Seq TRANSCRIPTOMIC cDNA Either total RNA was extracted directly with Trizol containing a capped RNA spike-in molecule, or cells were lysed on plate in 20 mM Tris pH 7.4; 150 mM NaCl; 5 mM MgCl2; 1% Triton X-100; 1 mM DTT; 100 ug/ml cycloheximide. Clarified lysates were then separated into a subpolysomal and polysomal fraction on a 5-50% sucrose gradient, and an equal amount of the capped spike-in RNA described above was added to each fraction. Total RNA was retrieved from both fractions by proteinase K treatment followed by acid phenol extraction. The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3' end of the cDNA was then ligated to a double stranded DNA linker with a 3' NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3' adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of Illumina sequences in a second PCR reaction. Illumina NovaSeq 6000 SRX13844957 GSM5827701_r1 SRP356002 PRJNA799027 SRS11722075 GSM5827701 GSM5827701 RNA-Seq TRANSCRIPTOMIC cDNA Either total RNA was extracted directly with Trizol containing a capped RNA spike-in molecule, or cells were lysed on plate in 20 mM Tris pH 7.4; 150 mM NaCl; 5 mM MgCl2; 1% Triton X-100; 1 mM DTT; 100 ug/ml cycloheximide. Clarified lysates were then separated into a subpolysomal and polysomal fraction on a 5-50% sucrose gradient, and an equal amount of the capped spike-in RNA described above was added to each fraction. Total RNA was retrieved from both fractions by proteinase K treatment followed by acid phenol extraction. The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3' end of the cDNA was then ligated to a double stranded DNA linker with a 3' NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3' adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of Illumina sequences in a second PCR reaction. Illumina NovaSeq 6000 SRX13844958 GSM5827702_r1 SRP356002 PRJNA799027 SRS11722076 GSM5827702 GSM5827702 RNA-Seq TRANSCRIPTOMIC cDNA Either total RNA was extracted directly with Trizol containing a capped RNA spike-in molecule, or cells were lysed on plate in 20 mM Tris pH 7.4; 150 mM NaCl; 5 mM MgCl2; 1% Triton X-100; 1 mM DTT; 100 ug/ml cycloheximide. Clarified lysates were then separated into a subpolysomal and polysomal fraction on a 5-50% sucrose gradient, and an equal amount of the capped spike-in RNA described above was added to each fraction. Total RNA was retrieved from both fractions by proteinase K treatment followed by acid phenol extraction. The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3' end of the cDNA was then ligated to a double stranded DNA linker with a 3' NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3' adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of Illumina sequences in a second PCR reaction. Illumina NovaSeq 6000 SRX13844959 GSM5827703_r1 SRP356002 PRJNA799027 SRS11722077 GSM5827703 GSM5827703 RNA-Seq TRANSCRIPTOMIC cDNA Either total RNA was extracted directly with Trizol containing a capped RNA spike-in molecule, or cells were lysed on plate in 20 mM Tris pH 7.4; 150 mM NaCl; 5 mM MgCl2; 1% Triton X-100; 1 mM DTT; 100 ug/ml cycloheximide. Clarified lysates were then separated into a subpolysomal and polysomal fraction on a 5-50% sucrose gradient, and an equal amount of the capped spike-in RNA described above was added to each fraction. Total RNA was retrieved from both fractions by proteinase K treatment followed by acid phenol extraction. The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3' end of the cDNA was then ligated to a double stranded DNA linker with a 3' NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3' adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of Illumina sequences in a second PCR reaction. Illumina NovaSeq 6000 SRX13844960 GSM5827704_r1 SRP356002 PRJNA799027 SRS11722078 GSM5827704 GSM5827704 RNA-Seq TRANSCRIPTOMIC cDNA Either total RNA was extracted directly with Trizol containing a capped RNA spike-in molecule, or cells were lysed on plate in 20 mM Tris pH 7.4; 150 mM NaCl; 5 mM MgCl2; 1% Triton X-100; 1 mM DTT; 100 ug/ml cycloheximide. Clarified lysates were then separated into a subpolysomal and polysomal fraction on a 5-50% sucrose gradient, and an equal amount of the capped spike-in RNA described above was added to each fraction. Total RNA was retrieved from both fractions by proteinase K treatment followed by acid phenol extraction. The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3' end of the cDNA was then ligated to a double stranded DNA linker with a 3' NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3' adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of Illumina sequences in a second PCR reaction. Illumina NovaSeq 6000 SRX13844961 GSM5827705_r1 SRP356002 PRJNA799027 SRS11722079 GSM5827705 GSM5827705 RNA-Seq GENOMIC cDNA Either total RNA was extracted directly with Trizol containing a capped RNA spike-in molecule, or cells were lysed on plate in 20 mM Tris pH 7.4; 150 mM NaCl; 5 mM MgCl2; 1% Triton X-100; 1 mM DTT; 100 ug/ml cycloheximide. Clarified lysates were then separated into a subpolysomal and polysomal fraction on a 5-50% sucrose gradient, and an equal amount of the capped spike-in RNA described above was added to each fraction. Total RNA was retrieved from both fractions by proteinase K treatment followed by acid phenol extraction. The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3' end of the cDNA was then ligated to a double stranded DNA linker with a 3' NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3' adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of Illumina sequences in a second PCR reaction. Illumina HiSeq 2000