SRX13864972 GSM5827227_r1 SRP356247 PRJNA798975 SRS11739003 GSM5827227 GSM5827227 RNA-Seq TRANSCRIPTOMIC cDNA Islets were gently dissociated into a single cell suspension in 500 μl Accutase (Innovative Cell Technologies) for up to 10 minutes at 37°C. Cells were then washed with PBS, passed through a 70 μm strainer and resuspended in a final volume of 200 μl. Cell viability and number of cells were determined using Trypan Blue Stain and a Countess Automated Cell Counter (ThermoFisher). 8,000 cells per sample were loaded onto the 10X Genomics Chromium Controller according to the 10X Genomics protocol. cDNA was prepared according to the 10X reverse transcriptase protocol as per manufacturer's instructions, and samples were stored at -80°C for concurrent library preparation and sequencing. Libraries were prepared according to the manufacturer's protocol for 10X Genomics Chromium Next GEM Single Cell V(D)J Reagent Kits v1.1. Libraries were pooled in equimolar concentration and sequenced to a targeted depth of 25,000 reads per cell with the Illumina NovaSeq SP 100 cycle kit with run parameters (read 1 28, index read i7 8, index read i5 0, read 2 60). Illumina NovaSeq 6000 SRX13864973 GSM5827228_r1 SRP356247 PRJNA798975 SRS11739002 GSM5827228 GSM5827228 RNA-Seq TRANSCRIPTOMIC cDNA Islets were gently dissociated into a single cell suspension in 500 μl Accutase (Innovative Cell Technologies) for up to 10 minutes at 37°C. Cells were then washed with PBS, passed through a 70 μm strainer and resuspended in a final volume of 200 μl. Cell viability and number of cells were determined using Trypan Blue Stain and a Countess Automated Cell Counter (ThermoFisher). 8,000 cells per sample were loaded onto the 10X Genomics Chromium Controller according to the 10X Genomics protocol. cDNA was prepared according to the 10X reverse transcriptase protocol as per manufacturer's instructions, and samples were stored at -80°C for concurrent library preparation and sequencing. Libraries were prepared according to the manufacturer's protocol for 10X Genomics Chromium Next GEM Single Cell V(D)J Reagent Kits v1.1. Libraries were pooled in equimolar concentration and sequenced to a targeted depth of 25,000 reads per cell with the Illumina NovaSeq SP 100 cycle kit with run parameters (read 1 28, index read i7 8, index read i5 0, read 2 60). Illumina NovaSeq 6000 SRX13864974 GSM5827229_r1 SRP356247 PRJNA798975 SRS11739004 GSM5827229 GSM5827229 RNA-Seq TRANSCRIPTOMIC cDNA Islets were gently dissociated into a single cell suspension in 500 μl Accutase (Innovative Cell Technologies) for up to 10 minutes at 37°C. Cells were then washed with PBS, passed through a 70 μm strainer and resuspended in a final volume of 200 μl. Cell viability and number of cells were determined using Trypan Blue Stain and a Countess Automated Cell Counter (ThermoFisher). 8,000 cells per sample were loaded onto the 10X Genomics Chromium Controller according to the 10X Genomics protocol. cDNA was prepared according to the 10X reverse transcriptase protocol as per manufacturer's instructions, and samples were stored at -80°C for concurrent library preparation and sequencing. Libraries were prepared according to the manufacturer's protocol for 10X Genomics Chromium Next GEM Single Cell V(D)J Reagent Kits v1.1. Libraries were pooled in equimolar concentration and sequenced to a targeted depth of 25,000 reads per cell with the Illumina NovaSeq SP 100 cycle kit with run parameters (read 1 28, index read i7 8, index read i5 0, read 2 60). Illumina NovaSeq 6000 SRX13864975 GSM5827230_r1 SRP356247 PRJNA798975 SRS11739006 GSM5827230 GSM5827230 RNA-Seq TRANSCRIPTOMIC cDNA Islets were gently dissociated into a single cell suspension in 500 μl Accutase (Innovative Cell Technologies) for up to 10 minutes at 37°C. Cells were then washed with PBS, passed through a 70 μm strainer and resuspended in a final volume of 200 μl. Cell viability and number of cells were determined using Trypan Blue Stain and a Countess Automated Cell Counter (ThermoFisher). 8,000 cells per sample were loaded onto the 10X Genomics Chromium Controller according to the 10X Genomics protocol. cDNA was prepared according to the 10X reverse transcriptase protocol as per manufacturer's instructions, and samples were stored at -80°C for concurrent library preparation and sequencing. Libraries were prepared according to the manufacturer's protocol for 10X Genomics Chromium Next GEM Single Cell V(D)J Reagent Kits v1.1. Libraries were pooled in equimolar concentration and sequenced to a targeted depth of 25,000 reads per cell with the Illumina NovaSeq SP 100 cycle kit with run parameters (read 1 28, index read i7 8, index read i5 0, read 2 60). Illumina NovaSeq 6000 SRX13864976 GSM5827231_r1 SRP356247 PRJNA798975 SRS11739005 GSM5827231 GSM5827231 RNA-Seq TRANSCRIPTOMIC cDNA Islets were gently dissociated into a single cell suspension in 500 μl Accutase (Innovative Cell Technologies) for up to 10 minutes at 37°C. Cells were then washed with PBS, passed through a 70 μm strainer and resuspended in a final volume of 200 μl. Cell viability and number of cells were determined using Trypan Blue Stain and a Countess Automated Cell Counter (ThermoFisher). 8,000 cells per sample were loaded onto the 10X Genomics Chromium Controller according to the 10X Genomics protocol. cDNA was prepared according to the 10X reverse transcriptase protocol as per manufacturer's instructions, and samples were stored at -80°C for concurrent library preparation and sequencing. Libraries were prepared according to the manufacturer's protocol for 10X Genomics Chromium Next GEM Single Cell V(D)J Reagent Kits v1.1. Libraries were pooled in equimolar concentration and sequenced to a targeted depth of 25,000 reads per cell with the Illumina NovaSeq SP 100 cycle kit with run parameters (read 1 28, index read i7 8, index read i5 0, read 2 60). Illumina NovaSeq 6000 SRX13864977 GSM5827232_r1 SRP356247 PRJNA798975 SRS11739007 GSM5827232 GSM5827232 RNA-Seq TRANSCRIPTOMIC cDNA Islets were gently dissociated into a single cell suspension in 500 μl Accutase (Innovative Cell Technologies) for up to 10 minutes at 37°C. Cells were then washed with PBS, passed through a 70 μm strainer and resuspended in a final volume of 200 μl. Cell viability and number of cells were determined using Trypan Blue Stain and a Countess Automated Cell Counter (ThermoFisher). 8,000 cells per sample were loaded onto the 10X Genomics Chromium Controller according to the 10X Genomics protocol. cDNA was prepared according to the 10X reverse transcriptase protocol as per manufacturer's instructions, and samples were stored at -80°C for concurrent library preparation and sequencing. Libraries were prepared according to the manufacturer's protocol for 10X Genomics Chromium Next GEM Single Cell V(D)J Reagent Kits v1.1. Libraries were pooled in equimolar concentration and sequenced to a targeted depth of 25,000 reads per cell with the Illumina NovaSeq SP 100 cycle kit with run parameters (read 1 28, index read i7 8, index read i5 0, read 2 60). Illumina NovaSeq 6000