SRX14049066 Bowland-1_VM_Enrichment 1 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883310 EN_1 Bowland-1_VM_Enrichment 1 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049067 Bowland-1_VM_Enrichment 2a SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883311 EN_2a Bowland-1_VM_Enrichment 2a AMPLICON GENOMIC PCR Illumina MiSeq SRX14049068 Bowland-1_VM_Enrichment 11 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883312 EN_11 Bowland-1_VM_Enrichment 11 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049069 Bowland-1_VM_Enrichment 12 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883313 EN_12 Bowland-1_VM_Enrichment 12 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049070 Bowland-1_VM_Enrichment Ex1 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883314 EN_Ex1 Bowland-1_VM_Enrichment Ex1 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049071 Bowland-1_VM_Enrichment Ex2 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883315 EN_Ex2 Bowland-1_VM_Enrichment Ex2 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049072 Bowland-1 60 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883316 Bowland_60 Bowland-1 60 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049073 Bowland-1 62 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883317 Bowland_62 Bowland-1 62 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049074 Bowland-1 65 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883318 Bowland_65 Bowland-1 65 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049075 Bowland-1 67 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883319 Bowland_67 Bowland-1 67 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049076 Bowland-1 68 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883320 Bowland_68 Bowland-1 68 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049077 Bowland-1 82* SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883321 Bowland_82* Bowland-1 82* AMPLICON GENOMIC PCR Illumina MiSeq SRX14049078 Bowland-1_VM_Enrichment 2b SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883322 EN_2b Bowland-1_VM_Enrichment 2b AMPLICON GENOMIC PCR Illumina MiSeq SRX14049079 Bowland-1 86 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883323 Bowland_86 Bowland-1 86 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049080 Bowland-1 Ex1 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883324 Ex1 Bowland-1 Ex1 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049081 Bowland-1 Ex2 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883325 Ex2 Bowland-1 Ex2 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049082 Bowland-1 Ex1* SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883326 Ex1* Bowland-1 Ex1* AMPLICON GENOMIC PCR Illumina MiSeq SRX14049083 Bowland-1 Ex2* SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883327 Ex2* Bowland-1 Ex2* AMPLICON GENOMIC PCR Illumina MiSeq SRX14049084 Bowland-1_VM_Enrichment 4 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883328 EN_4 Bowland-1_VM_Enrichment 4 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049085 Bowland-1_VM_Enrichment 5 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883329 EN_5 Bowland-1_VM_Enrichment 5 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049086 Bowland-1_VM_Enrichment 6 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883330 EN_6 Bowland-1_VM_Enrichment 6 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049087 Bowland-1_VM_Enrichment 7 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883331 EN_7 Bowland-1_VM_Enrichment 7 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049088 Bowland-1_VM_Enrichment 8 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883332 EN_8 Bowland-1_VM_Enrichment 8 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049089 Bowland-1_VM_Enrichment 9 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883333 EN_9 Bowland-1_VM_Enrichment 9 AMPLICON GENOMIC PCR Illumina MiSeq SRX14049090 Bowland-1_VM_Enrichment 10 SRP358466 bp0 Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2250-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep SRS11883334 EN_10 Bowland-1_VM_Enrichment 10 AMPLICON GENOMIC PCR Illumina MiSeq