SRX14071898 GSM5866832_r1 SRP358695 PRJNA804122 SRS11899289 GSM5866832 GSM5866832 ChIP-Seq GENOMIC ChIP Nuclear lysates were clarified from sonicated kidney organoids following dissociation using TrypLE. Immunoprecipitation of HNF1A protein was performed using HNF1A antibody (ab96777). Library preparation was done using a commercially available kit, NEB# E7645 NEBNext Ultra II DNA Library Prep Kit for Illumina KIT® following the manufacturer's protocol. During the library construction, the ChIP-ed DNA underwent End Repair, Adapter ligation, size selection (for ~150bp insert) and 16 cycles PCR enrichment to generate the final sequencing ready library. The quality of the library was checked using Agilent D1000 ScreenTape. The final library should have a fragment size of ~270bp insert plus adaptor and PCR primer sequences). The quality of the library was checked using Agilent D1000 ScreenTape. A single peak was expected and was observed indicating that the library was good and suitable for sequencing. NextSeq 500 SRX14071899 GSM5866833_r1 SRP358695 PRJNA804122 SRS11899290 GSM5866833 GSM5866833 ChIP-Seq GENOMIC ChIP Nuclear lysates were clarified from sonicated kidney organoids following dissociation using TrypLE. Immunoprecipitation of HNF1A protein was performed using HNF1A antibody (ab96777). Library preparation was done using a commercially available kit, NEB# E7645 NEBNext Ultra II DNA Library Prep Kit for Illumina KIT® following the manufacturer's protocol. During the library construction, the ChIP-ed DNA underwent End Repair, Adapter ligation, size selection (for ~150bp insert) and 16 cycles PCR enrichment to generate the final sequencing ready library. The quality of the library was checked using Agilent D1000 ScreenTape. The final library should have a fragment size of ~270bp insert plus adaptor and PCR primer sequences). The quality of the library was checked using Agilent D1000 ScreenTape. A single peak was expected and was observed indicating that the library was good and suitable for sequencing. NextSeq 500