SRX14091769 GSM5872469_r1 SRP358855 PRJNA804411 SRS11917256 GSM5872469 GSM5872469 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091770 GSM5872470_r1 SRP358855 PRJNA804411 SRS11917257 GSM5872470 GSM5872470 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091771 GSM5872471_r1 SRP358855 PRJNA804411 SRS11917258 GSM5872471 GSM5872471 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091772 GSM5872472_r1 SRP358855 PRJNA804411 SRS11917259 GSM5872472 GSM5872472 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091773 GSM5872473_r1 SRP358855 PRJNA804411 SRS11917260 GSM5872473 GSM5872473 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091774 GSM5872474_r1 SRP358855 PRJNA804411 SRS11917261 GSM5872474 GSM5872474 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091775 GSM5872475_r1 SRP358855 PRJNA804411 SRS11917262 GSM5872475 GSM5872475 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091776 GSM5872476_r1 SRP358855 PRJNA804411 SRS11917263 GSM5872476 GSM5872476 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091777 GSM5872485_r1 SRP358855 PRJNA804411 SRS11917264 GSM5872485 GSM5872485 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091778 GSM5872486_r1 SRP358855 PRJNA804411 SRS11917265 GSM5872486 GSM5872486 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091779 GSM5872487_r1 SRP358855 PRJNA804411 SRS11917266 GSM5872487 GSM5872487 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091780 GSM5872488_r1 SRP358855 PRJNA804411 SRS11917267 GSM5872488 GSM5872488 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091781 GSM5872489_r1 SRP358855 PRJNA804411 SRS11917268 GSM5872489 GSM5872489 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091782 GSM5872490_r1 SRP358855 PRJNA804411 SRS11917269 GSM5872490 GSM5872490 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091783 GSM5872491_r1 SRP358855 PRJNA804411 SRS11917270 GSM5872491 GSM5872491 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091784 GSM5872492_r1 SRP358855 PRJNA804411 SRS11917271 GSM5872492 GSM5872492 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091785 GSM5872517_r1 SRP358855 PRJNA804411 SRS11917272 GSM5872517 GSM5872517 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091786 GSM5872518_r1 SRP358855 PRJNA804411 SRS11917273 GSM5872518 GSM5872518 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091787 GSM5872519_r1 SRP358855 PRJNA804411 SRS11917274 GSM5872519 GSM5872519 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091788 GSM5872520_r1 SRP358855 PRJNA804411 SRS11917275 GSM5872520 GSM5872520 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091789 GSM5872521_r1 SRP358855 PRJNA804411 SRS11917276 GSM5872521 GSM5872521 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091790 GSM5872522_r1 SRP358855 PRJNA804411 SRS11917277 GSM5872522 GSM5872522 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091791 GSM5872523_r1 SRP358855 PRJNA804411 SRS11917278 GSM5872523 GSM5872523 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091792 GSM5872524_r1 SRP358855 PRJNA804411 SRS11917279 GSM5872524 GSM5872524 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091793 GSM5872549_r1 SRP358855 PRJNA804411 SRS11917280 GSM5872549 GSM5872549 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091794 GSM5872550_r1 SRP358855 PRJNA804411 SRS11917281 GSM5872550 GSM5872550 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091795 GSM5872551_r1 SRP358855 PRJNA804411 SRS11917282 GSM5872551 GSM5872551 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091796 GSM5872552_r1 SRP358855 PRJNA804411 SRS11917283 GSM5872552 GSM5872552 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091797 GSM5872553_r1 SRP358855 PRJNA804411 SRS11917284 GSM5872553 GSM5872553 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091798 GSM5872554_r1 SRP358855 PRJNA804411 SRS11917285 GSM5872554 GSM5872554 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091799 GSM5872555_r1 SRP358855 PRJNA804411 SRS11917286 GSM5872555 GSM5872555 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091800 GSM5872556_r1 SRP358855 PRJNA804411 SRS11917287 GSM5872556 GSM5872556 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091801 GSM5872573_r1 SRP358855 PRJNA804411 SRS11917288 GSM5872573 GSM5872573 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091802 GSM5872574_r1 SRP358855 PRJNA804411 SRS11917289 GSM5872574 GSM5872574 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091803 GSM5872575_r1 SRP358855 PRJNA804411 SRS11917290 GSM5872575 GSM5872575 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091804 GSM5872576_r1 SRP358855 PRJNA804411 SRS11917291 GSM5872576 GSM5872576 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091805 GSM5872577_r1 SRP358855 PRJNA804411 SRS11917292 GSM5872577 GSM5872577 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091806 GSM5872578_r1 SRP358855 PRJNA804411 SRS11917293 GSM5872578 GSM5872578 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091807 GSM5872579_r1 SRP358855 PRJNA804411 SRS11917294 GSM5872579 GSM5872579 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091808 GSM5872580_r1 SRP358855 PRJNA804411 SRS11917295 GSM5872580 GSM5872580 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091813 GSM5872609_r1 SRP358855 PRJNA804411 SRS11917300 GSM5872609 GSM5872609 OTHER GENOMIC other polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. NextSeq 550 SRX14091814 GSM5872610_r1 SRP358855 PRJNA804411 SRS11917301 GSM5872610 GSM5872610 OTHER GENOMIC other polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. NextSeq 550 SRX14091815 GSM5872611_r1 SRP358855 PRJNA804411 SRS11917302 GSM5872611 GSM5872611 OTHER GENOMIC other polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. NextSeq 550 SRX14091816 GSM5872477_r1 SRP358855 PRJNA804411 SRS11917303 GSM5872477 GSM5872477 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091817 GSM5872478_r1 SRP358855 PRJNA804411 SRS11917304 GSM5872478 GSM5872478 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091818 GSM5872479_r1 SRP358855 PRJNA804411 SRS11917305 GSM5872479 GSM5872479 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091819 GSM5872480_r1 SRP358855 PRJNA804411 SRS11917306 GSM5872480 GSM5872480 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091820 GSM5872481_r1 SRP358855 PRJNA804411 SRS11917307 GSM5872481 GSM5872481 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091821 GSM5872482_r1 SRP358855 PRJNA804411 SRS11917308 GSM5872482 GSM5872482 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091822 GSM5872483_r1 SRP358855 PRJNA804411 SRS11917309 GSM5872483 GSM5872483 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091823 GSM5872484_r1 SRP358855 PRJNA804411 SRS11917310 GSM5872484 GSM5872484 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091824 GSM5872509_r1 SRP358855 PRJNA804411 SRS11917311 GSM5872509 GSM5872509 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091825 GSM5872510_r1 SRP358855 PRJNA804411 SRS11917312 GSM5872510 GSM5872510 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091826 GSM5872511_r1 SRP358855 PRJNA804411 SRS11917313 GSM5872511 GSM5872511 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091827 GSM5872512_r1 SRP358855 PRJNA804411 SRS11917314 GSM5872512 GSM5872512 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091828 GSM5872513_r1 SRP358855 PRJNA804411 SRS11917315 GSM5872513 GSM5872513 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091829 GSM5872514_r1 SRP358855 PRJNA804411 SRS11917316 GSM5872514 GSM5872514 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091830 GSM5872515_r1 SRP358855 PRJNA804411 SRS11917317 GSM5872515 GSM5872515 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091831 GSM5872516_r1 SRP358855 PRJNA804411 SRS11917318 GSM5872516 GSM5872516 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091832 GSM5872541_r1 SRP358855 PRJNA804411 SRS11917319 GSM5872541 GSM5872541 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091833 GSM5872542_r1 SRP358855 PRJNA804411 SRS11917320 GSM5872542 GSM5872542 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091834 GSM5872543_r1 SRP358855 PRJNA804411 SRS11917321 GSM5872543 GSM5872543 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091835 GSM5872544_r1 SRP358855 PRJNA804411 SRS11917322 GSM5872544 GSM5872544 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091836 GSM5872545_r1 SRP358855 PRJNA804411 SRS11917323 GSM5872545 GSM5872545 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091837 GSM5872546_r1 SRP358855 PRJNA804411 SRS11917324 GSM5872546 GSM5872546 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091838 GSM5872547_r1 SRP358855 PRJNA804411 SRS11917325 GSM5872547 GSM5872547 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091839 GSM5872548_r1 SRP358855 PRJNA804411 SRS11917326 GSM5872548 GSM5872548 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091840 GSM5872565_r1 SRP358855 PRJNA804411 SRS11917327 GSM5872565 GSM5872565 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091841 GSM5872566_r1 SRP358855 PRJNA804411 SRS11917328 GSM5872566 GSM5872566 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091842 GSM5872567_r1 SRP358855 PRJNA804411 SRS11917329 GSM5872567 GSM5872567 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091843 GSM5872568_r1 SRP358855 PRJNA804411 SRS11917330 GSM5872568 GSM5872568 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091844 GSM5872569_r1 SRP358855 PRJNA804411 SRS11917331 GSM5872569 GSM5872569 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091845 GSM5872570_r1 SRP358855 PRJNA804411 SRS11917332 GSM5872570 GSM5872570 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091846 GSM5872571_r1 SRP358855 PRJNA804411 SRS11917333 GSM5872571 GSM5872571 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091847 GSM5872572_r1 SRP358855 PRJNA804411 SRS11917334 GSM5872572 GSM5872572 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091856 GSM5872493_r1 SRP358855 PRJNA804411 SRS11917343 GSM5872493 GSM5872493 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091857 GSM5872494_r1 SRP358855 PRJNA804411 SRS11917344 GSM5872494 GSM5872494 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091858 GSM5872495_r1 SRP358855 PRJNA804411 SRS11917345 GSM5872495 GSM5872495 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091859 GSM5872496_r1 SRP358855 PRJNA804411 SRS11917346 GSM5872496 GSM5872496 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091860 GSM5872497_r1 SRP358855 PRJNA804411 SRS11917347 GSM5872497 GSM5872497 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091861 GSM5872498_r1 SRP358855 PRJNA804411 SRS11917348 GSM5872498 GSM5872498 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091862 GSM5872499_r1 SRP358855 PRJNA804411 SRS11917349 GSM5872499 GSM5872499 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091863 GSM5872500_r1 SRP358855 PRJNA804411 SRS11917350 GSM5872500 GSM5872500 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091864 GSM5872525_r1 SRP358855 PRJNA804411 SRS11917351 GSM5872525 GSM5872525 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091865 GSM5872526_r1 SRP358855 PRJNA804411 SRS11917352 GSM5872526 GSM5872526 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091866 GSM5872527_r1 SRP358855 PRJNA804411 SRS11917353 GSM5872527 GSM5872527 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091867 GSM5872528_r1 SRP358855 PRJNA804411 SRS11917354 GSM5872528 GSM5872528 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091868 GSM5872529_r1 SRP358855 PRJNA804411 SRS11917355 GSM5872529 GSM5872529 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091869 GSM5872530_r1 SRP358855 PRJNA804411 SRS11917356 GSM5872530 GSM5872530 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091870 GSM5872531_r1 SRP358855 PRJNA804411 SRS11917357 GSM5872531 GSM5872531 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091871 GSM5872532_r1 SRP358855 PRJNA804411 SRS11917358 GSM5872532 GSM5872532 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091872 GSM5872557_r1 SRP358855 PRJNA804411 SRS11917359 GSM5872557 GSM5872557 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091873 GSM5872558_r1 SRP358855 PRJNA804411 SRS11917360 GSM5872558 GSM5872558 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091874 GSM5872559_r1 SRP358855 PRJNA804411 SRS11917361 GSM5872559 GSM5872559 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091875 GSM5872560_r1 SRP358855 PRJNA804411 SRS11917362 GSM5872560 GSM5872560 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091876 GSM5872561_r1 SRP358855 PRJNA804411 SRS11917363 GSM5872561 GSM5872561 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091877 GSM5872562_r1 SRP358855 PRJNA804411 SRS11917364 GSM5872562 GSM5872562 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091878 GSM5872563_r1 SRP358855 PRJNA804411 SRS11917365 GSM5872563 GSM5872563 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091879 GSM5872564_r1 SRP358855 PRJNA804411 SRS11917366 GSM5872564 GSM5872564 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091880 GSM5872581_r1 SRP358855 PRJNA804411 SRS11917367 GSM5872581 GSM5872581 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091881 GSM5872582_r1 SRP358855 PRJNA804411 SRS11917368 GSM5872582 GSM5872582 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091882 GSM5872583_r1 SRP358855 PRJNA804411 SRS11917369 GSM5872583 GSM5872583 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091883 GSM5872584_r1 SRP358855 PRJNA804411 SRS11917370 GSM5872584 GSM5872584 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091884 GSM5872585_r1 SRP358855 PRJNA804411 SRS11917371 GSM5872585 GSM5872585 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091885 GSM5872586_r1 SRP358855 PRJNA804411 SRS11917372 GSM5872586 GSM5872586 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091886 GSM5872587_r1 SRP358855 PRJNA804411 SRS11917373 GSM5872587 GSM5872587 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091888 GSM5872501_r1 SRP358855 PRJNA804411 SRS11917375 GSM5872501 GSM5872501 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091889 GSM5872502_r1 SRP358855 PRJNA804411 SRS11917376 GSM5872502 GSM5872502 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091890 GSM5872503_r1 SRP358855 PRJNA804411 SRS11917377 GSM5872503 GSM5872503 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091891 GSM5872504_r1 SRP358855 PRJNA804411 SRS11917378 GSM5872504 GSM5872504 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091892 GSM5872505_r1 SRP358855 PRJNA804411 SRS11917379 GSM5872505 GSM5872505 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091893 GSM5872506_r1 SRP358855 PRJNA804411 SRS11917380 GSM5872506 GSM5872506 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091894 GSM5872507_r1 SRP358855 PRJNA804411 SRS11917381 GSM5872507 GSM5872507 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091895 GSM5872508_r1 SRP358855 PRJNA804411 SRS11917382 GSM5872508 GSM5872508 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091896 GSM5872533_r1 SRP358855 PRJNA804411 SRS11917383 GSM5872533 GSM5872533 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091897 GSM5872534_r1 SRP358855 PRJNA804411 SRS11917384 GSM5872534 GSM5872534 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091898 GSM5872535_r1 SRP358855 PRJNA804411 SRS11917385 GSM5872535 GSM5872535 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091899 GSM5872536_r1 SRP358855 PRJNA804411 SRS11917386 GSM5872536 GSM5872536 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091900 GSM5872537_r1 SRP358855 PRJNA804411 SRS11917387 GSM5872537 GSM5872537 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091901 GSM5872538_r1 SRP358855 PRJNA804411 SRS11917388 GSM5872538 GSM5872538 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091902 GSM5872539_r1 SRP358855 PRJNA804411 SRS11917389 GSM5872539 GSM5872539 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000 SRX14091903 GSM5872540_r1 SRP358855 PRJNA804411 SRS11917390 GSM5872540 GSM5872540 RNA-Seq TRANSCRIPTOMIC cDNA polyA RNAs: Each oocyte was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. RiboMinus RNAs and ribosomal RNA: with Ovation Solo kit. CUT&Tag: oocytes were collected according to Bench top CUT&Tag V.3 protocol For polyA RNA seq: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit. For RiboMinus Total RNA-seq: single oocyte libraries were prepared according to the Ovation Solo system, but random hexamer primer is used for reverse transcription. For CUT&Tag sequencing: libraries were constructed according to the protocol Bench top CUT&Tag V.3, with fresh GV oocytes without light fixation. Illumina HiSeq 3000