SRX15010228 C1BC02 SRP372375 bp0 whole RNA purified by silica column; then mRNA purified by mRNA beads; reverse transcribed by SuperScript IV kit with random hexamers; full length VGSC CDS amplified by PCR with Q5 HiFi polymerase; amplicon purified by nuclease free water washes and concentration on a 100000 MWCO centrifugal filter; barcoding and sequencing library prep with NBD104 and LSK109; sequenced on a GridION using an R9.4 flowcell SRS12758940 ORL1952_C1BC02 C1BC02 AMPLICON TRANSCRIPTOMIC RT-PCR GridION SRX15010229 C1BC08 SRP372375 bp0 whole RNA purified by silica column; then mRNA purified by mRNA beads; reverse transcribed by SuperScript IV kit with random hexamers; full length VGSC CDS amplified by PCR with Q5 HiFi polymerase; amplicon purified by nuclease free water washes and concentration on a 100000 MWCO centrifugal filter; barcoding and sequencing library prep with NBD104 and LSK109; sequenced on a GridION using an R9.4 flowcell SRS12758941 VVFF_C01BC08 C1BC08 AMPLICON TRANSCRIPTOMIC RT-PCR GridION SRX15010230 C1BC11 SRP372375 bp0 whole RNA purified by silica column; then mRNA purified by mRNA beads; reverse transcribed by SuperScript IV kit with random hexamers; full length VGSC CDS amplified by PCR with Q5 HiFi polymerase; amplicon purified by nuclease free water washes and concentration on a 100000 MWCO centrifugal filter; barcoding and sequencing library prep with NBD104 and LSK109; sequenced on a GridION using an R9.4 flowcell SRS12758942 VVFC_C01BC11 C1BC11 AMPLICON TRANSCRIPTOMIC RT-PCR GridION SRX15010231 C2BC01 SRP372375 bp0 whole RNA purified by silica column; then mRNA purified by mRNA beads; reverse transcribed by SuperScript IV kit with random hexamers; full length VGSC CDS amplified by PCR with Q5 HiFi polymerase; amplicon purified by nuclease free water washes and concentration on a 100000 MWCO centrifugal filter; barcoding and sequencing library prep with NBD104 and LSK109; sequenced on a GridION using an R9.4 flowcell SRS12758943 VVCC_C02BC01 C2BC01 AMPLICON TRANSCRIPTOMIC RT-PCR GridION SRX15010232 C2BC04 SRP372375 bp0 whole RNA purified by silica column; then mRNA purified by mRNA beads; reverse transcribed by SuperScript IV kit with random hexamers; full length VGSC CDS amplified by PCR with Q5 HiFi polymerase; amplicon purified by nuclease free water washes and concentration on a 100000 MWCO centrifugal filter; barcoding and sequencing library prep with NBD104 and LSK109; sequenced on a GridION using an R9.4 flowcell SRS12758944 VIFC_C02BC04 C2BC04 AMPLICON TRANSCRIPTOMIC RT-PCR GridION SRX15010233 C2BC07 SRP372375 bp0 whole RNA purified by silica column; then mRNA purified by mRNA beads; reverse transcribed by SuperScript IV kit with random hexamers; full length VGSC CDS amplified by PCR with Q5 HiFi polymerase; amplicon purified by nuclease free water washes and concentration on a 100000 MWCO centrifugal filter; barcoding and sequencing library prep with NBD104 and LSK109; sequenced on a GridION using an R9.4 flowcell SRS12758945 VICC_C02BC07 C2BC07 AMPLICON TRANSCRIPTOMIC RT-PCR GridION SRX15010234 C2BC10 SRP372375 bp0 whole RNA purified by silica column; then mRNA purified by mRNA beads; reverse transcribed by SuperScript IV kit with random hexamers; full length VGSC CDS amplified by PCR with Q5 HiFi polymerase; amplicon purified by nuclease free water washes and concentration on a 100000 MWCO centrifugal filter; barcoding and sequencing library prep with NBD104 and LSK109; sequenced on a GridION using an R9.4 flowcell SRS12758946 IICC_C02BC10 C2BC10 AMPLICON TRANSCRIPTOMIC RT-PCR GridION SRX15010235 C1BC05 SRP372375 bp0 whole RNA purified by silica column; then mRNA purified by mRNA beads; reverse transcribed by SuperScript IV kit with random hexamers; full length VGSC CDS amplified by PCR with Q5 HiFi polymerase; amplicon purified by nuclease free water washes and concentration on a 100000 MWCO centrifugal filter; barcoding and sequencing library prep with NBD104 and LSK109; sequenced on a GridION using an R9.4 flowcell SRS12758947 PR_C01BC05 C1BC05 AMPLICON TRANSCRIPTOMIC RT-PCR GridION