SRX15013844 W_St_1_W2016_bc54 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762449 W_St_1_W2016_bc54 W_St_1_W2016_bc54 AMPLICON METAGENOMIC PCR Sequel SRX15013845 W_St_1_Sp2016_bc62 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762450 W_St_1_Sp2016_bc62 W_St_1_Sp2016_bc62 AMPLICON METAGENOMIC PCR Sequel SRX15013846 S_Da_1_W2016bc88 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762451 S_Da_1_W2016bc88 S_Da_1_W2016bc88 AMPLICON METAGENOMIC PCR Sequel SRX15013847 S_Da_1_Sp2016_bc96 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762452 S_Da_1_Sp2016_bc96 S_Da_1_Sp2016_bc96 AMPLICON METAGENOMIC PCR Sequel SRX15013848 S_Da_1_S2016_bc103 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762669 S_Da_1_S2016_bc103 S_Da_1_S2016_bc103 AMPLICON METAGENOMIC PCR Sequel SRX15013849 S_Da_1_A2016_bc110 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762453 S_Da_1_A2016_bc110 S_Da_1_A2016_bc110 AMPLICON METAGENOMIC PCR Sequel SRX15013850 W_St_2_W2016_bc55 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762454 W_St_2_W2016_bc55 W_St_2_W2016_bc55 AMPLICON METAGENOMIC PCR Sequel SRX15013851 S_Da_2_W2016_bc89 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762455 S_Da_2_W2016_bc89 S_Da_2_W2016_bc89 AMPLICON METAGENOMIC PCR Sequel SRX15013852 S_Da_2_Sp2016_bc97 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762456 S_Da_2_Sp2016_bc97 S_Da_2_Sp2016_bc97 AMPLICON METAGENOMIC PCR Sequel SRX15013853 S_Da_2_S2016_bc104 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762457 S_Da_2_S2016_bc104 S_Da_2_S2016_bc104 AMPLICON METAGENOMIC PCR Sequel SRX15013854 S_Da_2_A2016_bc111 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762458 S_Da_2_A2016_bc111 S_Da_2_A2016_bc111 AMPLICON METAGENOMIC PCR Sequel SRX15013855 S_Da_3_W2016_bc90 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762459 S_Da_3_W2016_bc90 S_Da_3_W2016_bc90 AMPLICON METAGENOMIC PCR Sequel SRX15013856 S_Da_3_Sp2016_bc98 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762460 S_Da_3_Sp2016_bc98 S_Da_3_Sp2016_bc98 AMPLICON METAGENOMIC PCR Sequel SRX15013857 S_Da_3_S2016_bc105 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762461 S_Da_3_S2016_bc105 S_Da_3_S2016_bc105 AMPLICON METAGENOMIC PCR Sequel SRX15013858 S_Da_3_A2016_bc112 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762462 S_Da_3_A2016_bc112 S_Da_3_A2016_bc112 AMPLICON METAGENOMIC PCR Sequel SRX15013859 W_HS_1_W2016_bc114 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762463 W_HS_1_W2016_bc114 W_HS_1_W2016_bc114 AMPLICON METAGENOMIC PCR Sequel SRX15013860 W_HS_1_Sp2016_bc118 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762464 W_HS_1_Sp2016_bc118 W_HS_1_Sp2016_bc118 AMPLICON METAGENOMIC PCR Sequel SRX15013861 W_St_2_Sp2016_bc63 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762465 W_St_2_Sp2016_bc63 W_St_2_Sp2016_bc63 AMPLICON METAGENOMIC PCR Sequel SRX15013862 W_St_3_S2016_bc72 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762466 W_St_3_S2016_bc72 W_St_3_S2016_bc72 AMPLICON METAGENOMIC PCR Sequel SRX15013863 S_WS_1_W2016_bc61 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762467 S_WS_1_W2016_bc61 S_WS_1_W2016_bc61 AMPLICON METAGENOMIC PCR Sequel SRX15013864 S_WS_1_Sp2016_bc99 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762468 S_WS_1_Sp2016_bc99 S_WS_1_Sp2016_bc99 AMPLICON METAGENOMIC PCR Sequel SRX15013865 S_WS_1_S2016_bc158 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762469 S_WS_1_S2016_bc158 S_WS_1_S2016_bc158 AMPLICON METAGENOMIC PCR Sequel SRX15013866 S_WS_1_A2016_bc109 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762470 S_WS_1_A2016_bc109 S_WS_1_A2016_bc109 AMPLICON METAGENOMIC PCR Sequel SRX15013867 S_WS_2_W2016_bc91 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762670 S_WS_2_W2016_bc91 S_WS_2_W2016_bc91 AMPLICON METAGENOMIC PCR Sequel SRX15013868 S_WS_2_Sp2016_bc69 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762471 S_WS_2_Sp2016_bc69 S_WS_2_Sp2016_bc69 AMPLICON METAGENOMIC PCR Sequel SRX15013869 S_WS_2_S2016_bc159 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762472 S_WS_2_S2016_bc159 S_WS_2_S2016_bc159 AMPLICON METAGENOMIC PCR Sequel SRX15013870 S_WS_2_A2016_bc113 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762473 S_WS_2_A2016_bc113 S_WS_2_A2016_bc113 AMPLICON METAGENOMIC PCR Sequel SRX15013871 KW_1_W2016_bc46 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762474 KW_1_W2016_bc46 KW_1_W2016_bc46 AMPLICON METAGENOMIC PCR Sequel SRX15013872 KW_1_Sp2016_bc48 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762475 KW_1_Sp2016_bc48 KW_1_Sp2016_bc48 AMPLICON METAGENOMIC PCR Sequel SRX15013873 W_St_3_A2016_bc80 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762476 W_St_3_A2016_bc80 W_St_3_A2016_bc80 AMPLICON METAGENOMIC PCR Sequel SRX15013874 KW_1_S2016_bc50 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762477 KW_1_S2016_bc50 KW_1_S2016_bc50 AMPLICON METAGENOMIC PCR Sequel SRX15013875 KW_1_A2016_bc52 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762478 KW_1_A2016_bc52 KW_1_A2016_bc52 AMPLICON METAGENOMIC PCR Sequel SRX15013876 KW_2_W2016_bc47 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762479 KW_2_W2016_bc47 KW_2_W2016_bc47 AMPLICON METAGENOMIC PCR Sequel SRX15013877 KW_2_Sp2016_bc49 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762480 KW_2_Sp2016_bc49 KW_2_Sp2016_bc49 AMPLICON METAGENOMIC PCR Sequel SRX15013878 S_HS_2_Sp2016_bc121 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762481 S_HS_2_Sp2016_bc121 S_HS_2_Sp2016_bc121 AMPLICON METAGENOMIC PCR Sequel SRX15013879 S_HS_2_S2016_bc126 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762482 S_HS_2_S2016_bc126 S_HS_2_S2016_bc126 AMPLICON METAGENOMIC PCR Sequel SRX15013880 S_HS_2_A2016_bc132 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762483 S_HS_2_A2016_bc132 S_HS_2_A2016_bc132 AMPLICON METAGENOMIC PCR Sequel SRX15013881 S_HS_3_W2016_bc155 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762484 S_HS_3_W2016_bc155 S_HS_3_W2016_bc155 AMPLICON METAGENOMIC PCR Sequel SRX15013882 S_HS_3_Sp2016_bc157 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762485 S_HS_3_Sp2016_bc157 S_HS_3_Sp2016_bc157 AMPLICON METAGENOMIC PCR Sequel SRX15013883 W_St_2_A2016_bc79 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762486 W_St_2_A2016_bc79 W_St_2_A2016_bc79 AMPLICON METAGENOMIC PCR Sequel SRX15013884 S_HS_3_S2016_bc127 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762487 S_HS_3_S2016_bc127 S_HS_3_S2016_bc127 AMPLICON METAGENOMIC PCR Sequel SRX15013885 S_HS_3_A2016_bc133 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762488 S_HS_3_A2016_bc133 S_HS_3_A2016_bc133 AMPLICON METAGENOMIC PCR Sequel SRX15013886 W_Mu_1_W2016_bc134 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762987 W_Mu_1_W2016_bc134 W_Mu_1_W2016_bc134 AMPLICON METAGENOMIC PCR Sequel SRX15013887 W_Mu_1_Sp2016_bc139 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762489 W_Mu_1_Sp2016_bc139 W_Mu_1_Sp2016_bc139 AMPLICON METAGENOMIC PCR Sequel SRX15013888 W_Mu_1_S2016_bc144 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762490 W_Mu_1_S2016_bc144 W_Mu_1_S2016_bc144 AMPLICON METAGENOMIC PCR Sequel SRX15013889 W_Mu_1_A2016_bc149 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762491 W_Mu_1_A2016_bc149 W_Mu_1_A2016_bc149 AMPLICON METAGENOMIC PCR Sequel SRX15013890 W_Mu_2_W2016_bc135 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762492 W_Mu_2_W2016_bc135 W_Mu_2_W2016_bc135 AMPLICON METAGENOMIC PCR Sequel SRX15013891 W_Mu_2_Sp2016_bc140 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762671 W_Mu_2_Sp2016_bc140 W_Mu_2_Sp2016_bc140 AMPLICON METAGENOMIC PCR Sequel SRX15013892 W_Mu_2_S2016_bc145 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762493 W_Mu_2_S2016_bc145 W_Mu_2_S2016_bc145 AMPLICON METAGENOMIC PCR Sequel SRX15013893 W_Mu_2_A2016_bc150 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762494 W_Mu_2_A2016_bc150 W_Mu_2_A2016_bc150 AMPLICON METAGENOMIC PCR Sequel SRX15013894 W_WS_1_S2016_bc73 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762495 W_WS_1_S2016_bc73 W_WS_1_S2016_bc73 AMPLICON METAGENOMIC PCR Sequel SRX15013895 W_WS_1_A2016_bc160 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762496 W_WS_1_A2016_bc160 W_WS_1_A2016_bc160 AMPLICON METAGENOMIC PCR Sequel SRX15013896 W_WS_2_W2016bc87 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762497 W_WS_2_W2016bc87 W_WS_2_W2016bc87 AMPLICON METAGENOMIC PCR Sequel SRX15013897 W_WS_2_Sp2016_bc65 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762498 W_WS_2_Sp2016_bc65 W_WS_2_Sp2016_bc65 AMPLICON METAGENOMIC PCR Sequel SRX15013898 W_WS_2_S2016_bc77 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762499 W_WS_2_S2016_bc77 W_WS_2_S2016_bc77 AMPLICON METAGENOMIC PCR Sequel SRX15013899 W_WS_2_A2016_bc161 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762500 W_WS_2_A2016_bc161 W_WS_2_A2016_bc161 AMPLICON METAGENOMIC PCR Sequel SRX15013900 KW_2_S2016_bc51 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762501 KW_2_S2016_bc51 KW_2_S2016_bc51 AMPLICON METAGENOMIC PCR Sequel SRX15013901 KW_2_A2016_bc53 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762502 KW_2_A2016_bc53 KW_2_A2016_bc53 AMPLICON METAGENOMIC PCR Sequel SRX15013902 NC_1_bc173 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762503 NC_1_bc173 NC_1_bc173 AMPLICON METAGENOMIC PCR Sequel SRX15013903 NC_2_bc174 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762504 NC_2_bc174 NC_2_bc174 AMPLICON METAGENOMIC PCR Sequel SRX15013904 NC_3_bc175 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762505 NC_3_bc175 NC_3_bc175 AMPLICON METAGENOMIC PCR Sequel SRX15013905 NC_4_bc176 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762506 NC_4_bc176 NC_4_bc176 AMPLICON METAGENOMIC PCR Sequel SRX15013906 S_St_1_W2016_bc58 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762507 S_St_1_W2016_bc58 S_St_1_W2016_bc58 AMPLICON METAGENOMIC PCR Sequel SRX15013907 S_St_1_Sp2016_bc66 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762508 S_St_1_Sp2016_bc66 S_St_1_Sp2016_bc66 AMPLICON METAGENOMIC PCR Sequel SRX15013908 S_St_1_S2016_bc74 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762509 S_St_1_S2016_bc74 S_St_1_S2016_bc74 AMPLICON METAGENOMIC PCR Sequel SRX15013909 S_St_1_A2016_bc81 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762510 S_St_1_A2016_bc81 S_St_1_A2016_bc81 AMPLICON METAGENOMIC PCR Sequel SRX15013910 S_St_2_W2016_bc59 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762511 S_St_2_W2016_bc59 S_St_2_W2016_bc59 AMPLICON METAGENOMIC PCR Sequel SRX15013911 S_St_2_Sp2016_bc67 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762512 S_St_2_Sp2016_bc67 S_St_2_Sp2016_bc67 AMPLICON METAGENOMIC PCR Sequel SRX15013912 S_St_2_S2016_bc75 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762513 S_St_2_S2016_bc75 S_St_2_S2016_bc75 AMPLICON METAGENOMIC PCR Sequel SRX15013913 S_St_2_A2016_bc78 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762514 S_St_2_A2016_bc78 S_St_2_A2016_bc78 AMPLICON METAGENOMIC PCR Sequel SRX15013914 W_St_1_S2016_bc70 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762515 W_St_1_S2016_bc70 W_St_1_S2016_bc70 AMPLICON METAGENOMIC PCR Sequel SRX15013915 S_St_3_W2016_bc60 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762516 S_St_3_W2016_bc60 S_St_3_W2016_bc60 AMPLICON METAGENOMIC PCR Sequel SRX15013916 W_HS_1_S2016_bc122 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762517 W_HS_1_S2016_bc122 W_HS_1_S2016_bc122 AMPLICON METAGENOMIC PCR Sequel SRX15013917 W_HS_1_A2016_bc128 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762518 W_HS_1_A2016_bc128 W_HS_1_A2016_bc128 AMPLICON METAGENOMIC PCR Sequel SRX15013918 W_HS_2_W2016_bc115 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762519 W_HS_2_W2016_bc115 W_HS_2_W2016_bc115 AMPLICON METAGENOMIC PCR Sequel SRX15013919 W_HS_2_Sp2016_bc119 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762930 W_HS_2_Sp2016_bc119 W_HS_2_Sp2016_bc119 AMPLICON METAGENOMIC PCR Sequel SRX15013920 W_HS_2_S2016_bc123 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762520 W_HS_2_S2016_bc123 W_HS_2_S2016_bc123 AMPLICON METAGENOMIC PCR Sequel SRX15013921 W_HS_2_A2016_bc129 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762521 W_HS_2_A2016_bc129 W_HS_2_A2016_bc129 AMPLICON METAGENOMIC PCR Sequel SRX15013922 W_HS_3_W2016_bc154 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762522 W_HS_3_W2016_bc154 W_HS_3_W2016_bc154 AMPLICON METAGENOMIC PCR Sequel SRX15013923 W_HS_3_Sp2016_bc156 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762523 W_HS_3_Sp2016_bc156 W_HS_3_Sp2016_bc156 AMPLICON METAGENOMIC PCR Sequel SRX15013924 W_HS_3_S2016_bc124 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762524 W_HS_3_S2016_bc124 W_HS_3_S2016_bc124 AMPLICON METAGENOMIC PCR Sequel SRX15013925 W_HS_3_A2016_bc130 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762525 W_HS_3_A2016_bc130 W_HS_3_A2016_bc130 AMPLICON METAGENOMIC PCR Sequel SRX15013926 W_St_2_S2016_bc71 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762526 W_St_2_S2016_bc71 W_St_2_S2016_bc71 AMPLICON METAGENOMIC PCR Sequel SRX15013927 S_HS_1_W2016_bc116 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762527 S_HS_1_W2016_bc116 S_HS_1_W2016_bc116 AMPLICON METAGENOMIC PCR Sequel SRX15013928 S_HS_1_Sp2016_bc120 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762528 S_HS_1_Sp2016_bc120 S_HS_1_Sp2016_bc120 AMPLICON METAGENOMIC PCR Sequel SRX15013929 S_HS_1_S2016_bc125 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762529 S_HS_1_S2016_bc125 S_HS_1_S2016_bc125 AMPLICON METAGENOMIC PCR Sequel SRX15013930 S_HS_1_A2016_bc131 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762530 S_HS_1_A2016_bc131 S_HS_1_A2016_bc131 AMPLICON METAGENOMIC PCR Sequel SRX15013931 S_HS_2_W2016_bc117 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762531 S_HS_2_W2016_bc117 S_HS_2_W2016_bc117 AMPLICON METAGENOMIC PCR Sequel SRX15013932 W_St_3_W2016_bc56 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762532 W_St_3_W2016_bc56 W_St_3_W2016_bc56 AMPLICON METAGENOMIC PCR Sequel SRX15013933 W_Mu_3_W2016_bc136 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762533 W_Mu_3_W2016_bc136 W_Mu_3_W2016_bc136 AMPLICON METAGENOMIC PCR Sequel SRX15013934 W_Mu_3_Sp2016_bc141 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762534 W_Mu_3_Sp2016_bc141 W_Mu_3_Sp2016_bc141 AMPLICON METAGENOMIC PCR Sequel SRX15013935 W_Mu_3_S2016_bc146 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762535 W_Mu_3_S2016_bc146 W_Mu_3_S2016_bc146 AMPLICON METAGENOMIC PCR Sequel SRX15013936 W_Mu_3_A2016_bc151 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762536 W_Mu_3_A2016_bc151 W_Mu_3_A2016_bc151 AMPLICON METAGENOMIC PCR Sequel SRX15013937 S_Mu_1_W2016_bc137 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762537 S_Mu_1_W2016_bc137 S_Mu_1_W2016_bc137 AMPLICON METAGENOMIC PCR Sequel SRX15013938 S_Mu_1_Sp2016_bc142 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762538 S_Mu_1_Sp2016_bc142 S_Mu_1_Sp2016_bc142 AMPLICON METAGENOMIC PCR Sequel SRX15013939 S_Mu_1_S2016_bc147 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762539 S_Mu_1_S2016_bc147 S_Mu_1_S2016_bc147 AMPLICON METAGENOMIC PCR Sequel SRX15013940 S_Mu_1_A2016_bc152 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762540 S_Mu_1_A2016_bc152 S_Mu_1_A2016_bc152 AMPLICON METAGENOMIC PCR Sequel SRX15013941 S_Mu_2_W2016_bc138 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762541 S_Mu_2_W2016_bc138 S_Mu_2_W2016_bc138 AMPLICON METAGENOMIC PCR Sequel SRX15013942 S_Mu_2_Sp2016_bc143 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762542 S_Mu_2_Sp2016_bc143 S_Mu_2_Sp2016_bc143 AMPLICON METAGENOMIC PCR Sequel SRX15013943 W_St_3_Sp2016_bc64 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762543 W_St_3_Sp2016_bc64 W_St_3_Sp2016_bc64 AMPLICON METAGENOMIC PCR Sequel SRX15013944 S_Mu_2_S2016_bc148 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762544 S_Mu_2_S2016_bc148 S_Mu_2_S2016_bc148 AMPLICON METAGENOMIC PCR Sequel SRX15013945 S_Mu_2_A2016_bc153 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762545 S_Mu_2_A2016_bc153 S_Mu_2_A2016_bc153 AMPLICON METAGENOMIC PCR Sequel SRX15013946 W_WS_1_W2016_bc57 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762546 W_WS_1_W2016_bc57 W_WS_1_W2016_bc57 AMPLICON METAGENOMIC PCR Sequel SRX15013947 W_WS_1_Sp2016_bc95 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762547 W_WS_1_Sp2016_bc95 W_WS_1_Sp2016_bc95 AMPLICON METAGENOMIC PCR Sequel SRX15013948 S_St_3_Sp2016_bc68 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762548 S_St_3_Sp2016_bc68 S_St_3_Sp2016_bc68 AMPLICON METAGENOMIC PCR Sequel SRX15013949 S_St_3_S2016_bc102 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762549 S_St_3_S2016_bc102 S_St_3_S2016_bc102 AMPLICON METAGENOMIC PCR Sequel SRX15013950 S_St_3_A2016_bc108 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762550 S_St_3_A2016_bc108 S_St_3_A2016_bc108 AMPLICON METAGENOMIC PCR Sequel SRX15013951 W_Da_1_W2016_bc84 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762551 W_Da_1_W2016_bc84 W_Da_1_W2016_bc84 AMPLICON METAGENOMIC PCR Sequel SRX15013952 W_Da_1_Sp2016_bc92 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762552 W_Da_1_Sp2016_bc92 W_Da_1_Sp2016_bc92 AMPLICON METAGENOMIC PCR Sequel SRX15013953 W_Da_1_S2016_bc100 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762553 W_Da_1_S2016_bc100 W_Da_1_S2016_bc100 AMPLICON METAGENOMIC PCR Sequel SRX15013954 W_Da_1_A2016_bc106 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762554 W_Da_1_A2016_bc106 W_Da_1_A2016_bc106 AMPLICON METAGENOMIC PCR Sequel SRX15013955 W_Da_2_W2016_bc85 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762555 W_Da_2_W2016_bc85 W_Da_2_W2016_bc85 AMPLICON METAGENOMIC PCR Sequel SRX15013956 W_Da_2_Sp2016_bc93 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762556 W_Da_2_Sp2016_bc93 W_Da_2_Sp2016_bc93 AMPLICON METAGENOMIC PCR Sequel SRX15013957 W_St_1_A2016_bc82 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762672 W_St_1_A2016_bc82 W_St_1_A2016_bc82 AMPLICON METAGENOMIC PCR Sequel SRX15013958 W_Da_2_S2016_bc101 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762557 W_Da_2_S2016_bc101 W_Da_2_S2016_bc101 AMPLICON METAGENOMIC PCR Sequel SRX15013959 W_Da_2_A2016_bc107 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762558 W_Da_2_A2016_bc107 W_Da_2_A2016_bc107 AMPLICON METAGENOMIC PCR Sequel SRX15013960 W_Da_3_W2016_bc86 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762559 W_Da_3_W2016_bc86 W_Da_3_W2016_bc86 AMPLICON METAGENOMIC PCR Sequel SRX15013961 W_Da_3_Sp2016_bc94 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762560 W_Da_3_Sp2016_bc94 W_Da_3_Sp2016_bc94 AMPLICON METAGENOMIC PCR Sequel SRX15013962 W_Da_3_S2016_bc76 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762561 W_Da_3_S2016_bc76 W_Da_3_S2016_bc76 AMPLICON METAGENOMIC PCR Sequel SRX15013963 W_Da_3_A2016_bc83 SRP372457 bp0 Water samples were collected on Sterivex filters (EMD Millipore, Darmstadt, Germany) and extracted using QIAamp DNA mini kit (Qiagen, Hilden, Germany) while sediment samples were extracted directly using the NucleoSpin Soil kit (Macherey Nagel, Dren, Germany). The whole 16S rRNA genes was amplified using 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3) primers using MyFiTM Mix (Bioline, London, UK) and the following PCR steps: 95C for 3 min, 25 cycles of 95C for 30 s, 57C for 30 s and 72C for 60 s with a final elongation step at 72C for 3 min. Concentration and quality of products were determined using a TapeStation 4200. Samples were purified with the Agencourt AMPure XP kit (Beckman Coulter, USA), sequencing libraries were prepared using the SMRTbell Template Prep Kit 1.0SPv3. The Sequel Binding Kit 2.0 (Pacific Biosciences, USA) was used to bind DNA template libraries to the Sequel polymerase 2.0. Sequencing data was collected in Sequel SMRT Cells 1M v2 with 600 min movie time on the Sequel system I (Pacific Biosciences, USA). SRS12762562 W_Da_3_A2016_bc83 W_Da_3_A2016_bc83 AMPLICON METAGENOMIC PCR Sequel