SRP372498 PRJNA832676 GSE201684 Detection of gene expression level of Yersinia pestis vaccine strain EV76 at 21 and 37 degrees Celsius In order to study the gene expression differences of ev76 in the environment and the appropriate growth temperature of flea (21 ?) and mouse body temperature (37 ?), we cultured ev76 at 21 ? and 37 ? respectively, collected bacterial precipitation and sequenced the transcriptome Overall design: The experiment was divided into 21 ? group and 37 ? group. Each group was set with three parallel controls: A, B and C GSE201684