SRX15036832 GSM6070797_r1 SRP372608 PRJNA832879 SRS12783676 GSM6070797 GSM6070797 RNA-Seq TRANSCRIPTOMIC cDNA For each nuclear sample, ventricular heart tissue was isolated from mice at 18 weeks of age. CM nuclei were isolated as previously described by Cui and Olson (STAR Protocols, 2021).Isolated nuclei were immediately used for downstream processing, or stored in Nuclei PURE Storage Buffer (Sigma Aldrich) at -80 °C. RNA was isolated using the RNeasy Micro Kit (Qiagen) RNA-seq libraries were generated using the SMARTer Stranded Total RNA-Seq Kit v2-Pico Input Mammalian kit (Takara), containing Illumina sequencing adapters. Libraries were visualized on a 2200 TapeStation System (Agilent) and quantified by QuBit dsDNA high-sensitivity assay (Invitrogen) before pooling and loading onto an Illumina NextSeq 500. NextSeq 500 SRX15036833 GSM6070798_r1 SRP372608 PRJNA832879 SRS12783675 GSM6070798 GSM6070798 RNA-Seq TRANSCRIPTOMIC cDNA For each nuclear sample, ventricular heart tissue was isolated from mice at 18 weeks of age. CM nuclei were isolated as previously described by Cui and Olson (STAR Protocols, 2021).Isolated nuclei were immediately used for downstream processing, or stored in Nuclei PURE Storage Buffer (Sigma Aldrich) at -80 °C. RNA was isolated using the RNeasy Micro Kit (Qiagen) RNA-seq libraries were generated using the SMARTer Stranded Total RNA-Seq Kit v2-Pico Input Mammalian kit (Takara), containing Illumina sequencing adapters. Libraries were visualized on a 2200 TapeStation System (Agilent) and quantified by QuBit dsDNA high-sensitivity assay (Invitrogen) before pooling and loading onto an Illumina NextSeq 500. NextSeq 500 SRX15036834 GSM6070799_r1 SRP372608 PRJNA832879 SRS12783677 GSM6070799 GSM6070799 RNA-Seq TRANSCRIPTOMIC cDNA For each nuclear sample, ventricular heart tissue was isolated from mice at 18 weeks of age. CM nuclei were isolated as previously described by Cui and Olson (STAR Protocols, 2021).Isolated nuclei were immediately used for downstream processing, or stored in Nuclei PURE Storage Buffer (Sigma Aldrich) at -80 °C. RNA was isolated using the RNeasy Micro Kit (Qiagen) RNA-seq libraries were generated using the SMARTer Stranded Total RNA-Seq Kit v2-Pico Input Mammalian kit (Takara), containing Illumina sequencing adapters. Libraries were visualized on a 2200 TapeStation System (Agilent) and quantified by QuBit dsDNA high-sensitivity assay (Invitrogen) before pooling and loading onto an Illumina NextSeq 500. NextSeq 500