16s rDNA sequencing of cecal contents: five weeks C57BL/6J male mouse

SRX15040112 Con_1.1 16s rDNA sequencing of cecal contents: five weeks C57BL/6J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt2 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786723 LTCon_1.1.fq Con_1.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040113 RG_1.1 16s rDNA sequencing of cecal contents: five weeks C57BL/16J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt12 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786724 LTRG_1.1.fq RG_1.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040114 RG_2.1 16s rDNA sequencing of cecal contents: five weeks C57BL/18J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt14 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786725 LTRG_2.1.fq RG_2.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040115 RG_3.1 16s rDNA sequencing of cecal contents: five weeks C57BL/20J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt16 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786726 LTRG_3.1.fq RG_3.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040116 RG_4.1 16s rDNA sequencing of cecal contents: five weeks C57BL/22J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt18 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786727 LTRG_4.1.fq RG_4.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040117 RG_5.1 16s rDNA sequencing of cecal contents: five weeks C57BL/24J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt20 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786728 LTRG_5.1.fq RG_5.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040118 Con_2.1 16s rDNA sequencing of cecal contents: five weeks C57BL/8J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt4 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786729 LTCon_2.1.fq Con_2.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040119 GJ_1.1 16s rDNA sequencing of cecal contents: five weeks C57BL/26J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt22 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786730 LTGJ_1.1.fq GJ_1.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040120 GJ_2.1 16s rDNA sequencing of cecal contents: five weeks C57BL/28J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt24 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786731 LTGJ_2.1.fq GJ_2.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040121 GJ_3.1 16s rDNA sequencing of cecal contents: five weeks C57BL/30J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt26 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786732 LTGJ_3.1.fq GJ_3.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040122 GJ_4.1 16s rDNA sequencing of cecal contents: five weeks C57BL/32J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt28 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786733 LTGJ_4.1.fq GJ_4.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040123 GJ_5.1 16s rDNA sequencing of cecal contents: five weeks C57BL/34J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt30 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786734 LTGJ_5.1.fq GJ_5.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040124 FZ_1.1 16s rDNA sequencing of cecal contents: five weeks C57BL/36J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt32 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786735 LTFZ_1.1.fq FZ_1.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040125 FZ_2.1 16s rDNA sequencing of cecal contents: five weeks C57BL/38J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt34 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786736 LTFZ_2.1.fq FZ_2.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040126 FZ_3.1 16s rDNA sequencing of cecal contents: five weeks C57BL/40J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt36 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786737 LTFZ_3.1.fq FZ_3.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040127 FZ_4.1 16s rDNA sequencing of cecal contents: five weeks C57BL/42J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt38 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786738 LTFZ_4.1.fq FZ_4.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040128 FZ_5.1 16s rDNA sequencing of cecal contents: five weeks C57BL/44J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt40 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786739 LTFZ_5.1.fq FZ_5.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040129 Con_3.1 16s rDNA sequencing of cecal contents: five weeks C57BL/10J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt6 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786740 LTCon_3.1.fq Con_3.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040130 DH_1.1 16s rDNA sequencing of cecal contents: five weeks C57BL/46J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt42 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786741 LTDH_1.1.fq DH_1.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040131 DH_2.1 16s rDNA sequencing of cecal contents: five weeks C57BL/48J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt44 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786742 LTDH_2.1.fq DH_2.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040132 DH_3.1 16s rDNA sequencing of cecal contents: five weeks C57BL/50J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt46 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786743 LTDH_3.1.fq DH_3.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040133 DH_4.1 16s rDNA sequencing of cecal contents: five weeks C57BL/52J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt48 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786744 LTDH_4.1.fq DH_4.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040134 DH_5.1 16s rDNA sequencing of cecal contents: five weeks C57BL/54J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt50 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786745 LTDH_5.1.fq DH_5.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040135 HQ_1.1 16s rDNA sequencing of cecal contents: five weeks C57BL/56J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt52 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786746 LTHQ_1.1.fq HQ_1.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040136 HQ_2.1 16s rDNA sequencing of cecal contents: five weeks C57BL/58J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt54 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786747 LTHQ_2.1.fq HQ_2.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040137 HQ_3.1 16s rDNA sequencing of cecal contents: five weeks C57BL/60J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt56 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786748 LTHQ_3.1.fq HQ_3.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040138 HQ_4.1 16s rDNA sequencing of cecal contents: five weeks C57BL/62J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt58 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786749 LTHQ_4.1.fq HQ_4.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040139 HQ_5.1 16s rDNA sequencing of cecal contents: five weeks C57BL/64J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt60 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786750 LTHQ_5.1.fq HQ_5.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040140 Con_4.1 16s rDNA sequencing of cecal contents: five weeks C57BL/12J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt8 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786751 LTCon_4.1.fq Con_4.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040141 HL_1.1 16s rDNA sequencing of cecal contents: five weeks C57BL/66J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt62 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786752 LTHL_1.1.fq HL_1.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040142 HL_2.1 16s rDNA sequencing of cecal contents: five weeks C57BL/68J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt64 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786753 LTHL_2.1.fq HL_2.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040143 HL_3.1 16s rDNA sequencing of cecal contents: five weeks C57BL/70J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt66 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786754 LTHL_3.1.fq HL_3.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040144 HL_4.1 16s rDNA sequencing of cecal contents: five weeks C57BL/72J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt68 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786755 LTHL_4.1.fq HL_4.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040145 HL_5.1 16s rDNA sequencing of cecal contents: five weeks C57BL/74J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt70 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786756 LTHL_5.1.fq HL_5.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000 SRX15040146 Con_5.1 16s rDNA sequencing of cecal contents: five weeks C57BL/14J male mouse SRP372719 bp0 Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt10 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) SRS12786757 LTCon_5.1.fq Con_5.1 AMPLICON METAGENOMIC PCR Illumina HiSeq 3000