SRX15044704 C.sim_minion_run_1 SRP372582 PRJNA832799 mRNA extraction was conducted with NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). cDNA was synthesized with Mint2 kit (Evrogen). cDNA sequencing carried out on MinION Mk1B platform (Oxford Nanopore Technologies) with Direct cDNA Sequencing Kit SQK-DCS109. Basecalling of raw data was conducted with guppy basecaller. SRS12791070 minion_C.sim_run1 C.sim_minion_run_1 RNA-Seq TRANSCRIPTOMIC PCR MinION