SRX15045209 GSM6076934_r1 SRP372759 PRJNA833148 SRS12791336 GSM6076934 GSM6076934 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045210 GSM6076935_r1 SRP372759 PRJNA833148 SRS12791531 GSM6076935 GSM6076935 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045211 GSM6076936_r1 SRP372759 PRJNA833148 SRS12791337 GSM6076936 GSM6076936 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045212 GSM6076937_r1 SRP372759 PRJNA833148 SRS12791532 GSM6076937 GSM6076937 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045213 GSM6076938_r1 SRP372759 PRJNA833148 SRS12791338 GSM6076938 GSM6076938 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045214 GSM6076939_r1 SRP372759 PRJNA833148 SRS12791339 GSM6076939 GSM6076939 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500