SRX15045318 GSM6076888_r1 SRP372766 PRJNA833149 SRS12791475 GSM6076888 GSM6076888 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045319 GSM6076889_r1 SRP372766 PRJNA833149 SRS12791473 GSM6076889 GSM6076889 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045320 GSM6076890_r1 SRP372766 PRJNA833149 SRS12791474 GSM6076890 GSM6076890 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045321 GSM6076891_r1 SRP372766 PRJNA833149 SRS12791477 GSM6076891 GSM6076891 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045322 GSM6076892_r1 SRP372766 PRJNA833149 SRS12791476 GSM6076892 GSM6076892 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045323 GSM6076893_r1 SRP372766 PRJNA833149 SRS12791478 GSM6076893 GSM6076893 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045324 GSM6076894_r1 SRP372766 PRJNA833149 SRS12791479 GSM6076894 GSM6076894 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045325 GSM6076895_r1 SRP372766 PRJNA833149 SRS12791480 GSM6076895 GSM6076895 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045326 GSM6076896_r1 SRP372766 PRJNA833149 SRS12791481 GSM6076896 GSM6076896 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045327 GSM6076897_r1 SRP372766 PRJNA833149 SRS12791482 GSM6076897 GSM6076897 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045328 GSM6076898_r1 SRP372766 PRJNA833149 SRS12791483 GSM6076898 GSM6076898 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045329 GSM6076899_r1 SRP372766 PRJNA833149 SRS12791484 GSM6076899 GSM6076899 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045330 GSM6076900_r1 SRP372766 PRJNA833149 SRS12791485 GSM6076900 GSM6076900 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045331 GSM6076901_r1 SRP372766 PRJNA833149 SRS12791486 GSM6076901 GSM6076901 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045332 GSM6076902_r1 SRP372766 PRJNA833149 SRS12791487 GSM6076902 GSM6076902 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045333 GSM6076903_r1 SRP372766 PRJNA833149 SRS12791488 GSM6076903 GSM6076903 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045334 GSM6076920_r1 SRP372766 PRJNA833149 SRS12791489 GSM6076920 GSM6076920 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045335 GSM6076921_r1 SRP372766 PRJNA833149 SRS12791490 GSM6076921 GSM6076921 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045336 GSM6076922_r1 SRP372766 PRJNA833149 SRS12791491 GSM6076922 GSM6076922 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045337 GSM6076923_r1 SRP372766 PRJNA833149 SRS12791492 GSM6076923 GSM6076923 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045338 GSM6076924_r1 SRP372766 PRJNA833149 SRS12791493 GSM6076924 GSM6076924 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045339 GSM6076925_r1 SRP372766 PRJNA833149 SRS12791494 GSM6076925 GSM6076925 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045340 GSM6076926_r1 SRP372766 PRJNA833149 SRS12791495 GSM6076926 GSM6076926 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045341 GSM6076927_r1 SRP372766 PRJNA833149 SRS12791496 GSM6076927 GSM6076927 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045342 GSM6076928_r1 SRP372766 PRJNA833149 SRS12791497 GSM6076928 GSM6076928 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045343 GSM6076929_r1 SRP372766 PRJNA833149 SRS12791499 GSM6076929 GSM6076929 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045344 GSM6076930_r1 SRP372766 PRJNA833149 SRS12791498 GSM6076930 GSM6076930 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045345 GSM6076931_r1 SRP372766 PRJNA833149 SRS12791500 GSM6076931 GSM6076931 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045346 GSM6076932_r1 SRP372766 PRJNA833149 SRS12791501 GSM6076932 GSM6076932 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045347 GSM6076933_r1 SRP372766 PRJNA833149 SRS12791503 GSM6076933 GSM6076933 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045348 GSM6076904_r1 SRP372766 PRJNA833149 SRS12791502 GSM6076904 GSM6076904 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045349 GSM6076905_r1 SRP372766 PRJNA833149 SRS12791504 GSM6076905 GSM6076905 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045350 GSM6076906_r1 SRP372766 PRJNA833149 SRS12791505 GSM6076906 GSM6076906 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045351 GSM6076907_r1 SRP372766 PRJNA833149 SRS12791506 GSM6076907 GSM6076907 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045352 GSM6076908_r1 SRP372766 PRJNA833149 SRS12791507 GSM6076908 GSM6076908 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045353 GSM6076909_r1 SRP372766 PRJNA833149 SRS12791508 GSM6076909 GSM6076909 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045354 GSM6076910_r1 SRP372766 PRJNA833149 SRS12791509 GSM6076910 GSM6076910 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045355 GSM6076911_r1 SRP372766 PRJNA833149 SRS12791510 GSM6076911 GSM6076911 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045356 GSM6076912_r1 SRP372766 PRJNA833149 SRS12791512 GSM6076912 GSM6076912 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045357 GSM6076913_r1 SRP372766 PRJNA833149 SRS12791511 GSM6076913 GSM6076913 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045358 GSM6076914_r1 SRP372766 PRJNA833149 SRS12791513 GSM6076914 GSM6076914 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045359 GSM6076915_r1 SRP372766 PRJNA833149 SRS12791514 GSM6076915 GSM6076915 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045360 GSM6076916_r1 SRP372766 PRJNA833149 SRS12791515 GSM6076916 GSM6076916 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045361 GSM6076917_r1 SRP372766 PRJNA833149 SRS12791516 GSM6076917 GSM6076917 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045362 GSM6076918_r1 SRP372766 PRJNA833149 SRS12791517 GSM6076918 GSM6076918 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500 SRX15045363 GSM6076919_r1 SRP372766 PRJNA833149 SRS12791518 GSM6076919 GSM6076919 RNA-Seq TRANSCRIPTOMIC cDNA Total RNAs were isolated using the RNeasy Mini Kit (Qiagen), following the instructions provided by the manufacturer. Nascent EU-RNAs were biotinylated using bisulfide biotin azide via click reaction and purified using streptavidin beads following the instructions provided by the Click-iTTM Nascent RNA Capture Kit (Thermo Fisher Scientific, Cat# C10365). The cDNA libraries for purified ascent transcripts were prepared using the Universal RNA-seq with NuQuant® kit (NuGEN, Cat# 0364), following the manual provided by the manufacturer. Ribosomal RNAs were depleted using the custom designed AnyDeplete Probe Mix for Xenopus laevis provided by the kit. NextSeq 500