SRX15157657 Pv_SRNF3_S39 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900073 Mature_Nodules Pv_SRNF3_S39 miRNA-Seq TRANSCRIPTOMIC size fractionation NextSeq 500 SRX15157658 4_Raiz_EV_Rhizobio_GFL4_S22 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900074 Roots_Rhizobia_EV 4_Raiz_EV_Rhizobio_GFL4_S22 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157659 3_Raiz_EV_Mock2_GFL3_S21 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900075 Roots_Mock_EV 3_Raiz_EV_Mock2_GFL3_S21 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157660 2_Raiz_EV_Mock2_GFL2_S20 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900075 Roots_Mock_EV 2_Raiz_EV_Mock2_GFL2_S20 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157661 Pv_SRNF1_S37 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900073 Mature_Nodules Pv_SRNF1_S37 miRNA-Seq TRANSCRIPTOMIC size fractionation NextSeq 500 SRX15157662 1_Raiz_EV_Mock1_GFL1_S19 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900075 Roots_Mock_EV 1_Raiz_EV_Mock1_GFL1_S19 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157663 24_Nodulos21d_AGO5_3_GFL24_S42 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900076 Mature_Nodules_AGO5RNAi 24_Nodulos21d_AGO5_3_GFL24_S42 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157664 23_Nodulos21d_AGO5_2_GFL23_S41 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900076 Mature_Nodules_AGO5RNAi 23_Nodulos21d_AGO5_2_GFL23_S41 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157665 22_Nodulos21d_AGO5_1_GFL22_S40 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900076 Mature_Nodules_AGO5RNAi 22_Nodulos21d_AGO5_1_GFL22_S40 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157666 Pv_SRNF2_S38 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900073 Mature_Nodules Pv_SRNF2_S38 miRNA-Seq TRANSCRIPTOMIC size fractionation NextSeq 500 SRX15157667 20_Nodulos21d_EV2_GFL20_S38 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900077 Mature_Nodules_EV 20_Nodulos21d_EV2_GFL20_S38 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157668 19_Nodulos21d_EV1_GFL19_S37 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900077 Mature_Nodules_EV 19_Nodulos21d_EV1_GFL19_S37 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157669 15_Nodulos10d_EV3_GFL15_S33 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900078 Nodule_Primordia_EV 15_Nodulos10d_EV3_GFL15_S33 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157670 14_Nodulos10d_EV2_GFL14_S32 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900078 Nodule_Primordia_EV 14_Nodulos10d_EV2_GFL14_S32 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157671 13_Nodulos10d_EV1_GFL13_S31 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900078 Nodule_Primordia_EV 13_Nodulos10d_EV1_GFL13_S31 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157672 6_Raiz_EV_Rhizobio3_GFL6_S24 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900074 Roots_Rhizobia_EV 6_Raiz_EV_Rhizobio3_GFL6_S24 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157673 5_Raiz_EV_Rhizobio2_GFL5_S23 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900074 Roots_Rhizobia_EV 5_Raiz_EV_Rhizobio2_GFL5_S23 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157674 18_Nodulos10d_AGO5_3_GFL18_S36 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900079 Nodule_Primordia_AGO5RNAi 18_Nodulos10d_AGO5_3_GFL18_S36 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157675 17_Nodulos10d_AGO5_2_GFL17_S35 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900079 Nodule_Primordia_AGO5RNAi 17_Nodulos10d_AGO5_2_GFL17_S35 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157676 16_Nodulos10d_AGO5_1_GFL16_S34 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900079 Nodule_Primordia_AGO5RNAi 16_Nodulos10d_AGO5_1_GFL16_S34 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157677 12_Raiz_AGO5_Rhizobio3_GFL12_S30 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900080 Roots_Rhizobia_AGO5RNAi 12_Raiz_AGO5_Rhizobio3_GFL12_S30 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157678 11_Raiz_AGO5_Rhizobio2_GFL11_S29 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900080 Roots_Rhizobia_AGO5RNAi 11_Raiz_AGO5_Rhizobio2_GFL11_S29 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157679 10_Raiz_AGO5_Rhizobio1_GFL10_S28 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900080 Roots_Rhizobia_AGO5RNAi 10_Raiz_AGO5_Rhizobio1_GFL10_S28 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157680 Pv_SRP3_S36 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900081 Nodule_Primordia Pv_SRP3_S36 miRNA-Seq TRANSCRIPTOMIC size fractionation NextSeq 500 SRX15157681 9_Raiz_AGO5_Mock3_GFL9_S27 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900082 Roots_Mock_AGO5RNAi 9_Raiz_AGO5_Mock3_GFL9_S27 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157682 8_Raiz_AGO5_Mock2_GFL8_S26 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900082 Roots_Mock_AGO5RNAi 8_Raiz_AGO5_Mock2_GFL8_S26 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157683 7_Raiz_AGO5_Mock1_GFL7_S25 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900082 Roots_Mock_AGO5RNAi 7_Raiz_AGO5_Mock1_GFL7_S25 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500 SRX15157684 Pv_SRP2_S35 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900081 Nodule_Primordia Pv_SRP2_S35 miRNA-Seq TRANSCRIPTOMIC size fractionation NextSeq 500 SRX15157685 Pv_SRP1_S34 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900081 Nodule_Primordia Pv_SRP1_S34 miRNA-Seq TRANSCRIPTOMIC size fractionation NextSeq 500 SRX15157686 Pv_SRC3_S33 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900083 Roots_Mock Pv_SRC3_S33 miRNA-Seq TRANSCRIPTOMIC size fractionation NextSeq 500 SRX15157687 Pv_SRC2_S32 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900083 Roots_Mock Pv_SRC2_S32 miRNA-Seq TRANSCRIPTOMIC size fractionation NextSeq 500 SRX15157688 Pv_SRC1_S31 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900083 Roots_Mock Pv_SRC1_S31 miRNA-Seq TRANSCRIPTOMIC size fractionation NextSeq 500 SRX15157689 21_Nodulos21d_EV3_GFL21_S39 SRP373676 bp0 Total RNA was isolated as described in the previous section from 0.5 g of one-day rhizobia- or mock-inoculated transgenic roots, transgenic roots bearing nodule primordia, and transgenic mature nodules expressing a control vector, or the PvAGO5-RNAi construct was isolated as described in the previous section. Stranded messenger RNA-seq (mRNA-seq) libraries were generated from 1 ?g of gDNA-free total RNA from each experimental condition and prepared using the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions.In total, 24-stranded mRNA-seq libraries (transgenic roots expressing an empty vector or the PvAGO5-RNAi construct and inoculated for one day with mock or rhizobia; transgenic roots with nodule primordia expressing the empty vector of the PvAGO5-RNAi construct, and mature nodules from roots expressing the control vector or the PvAGO5-RNAi) were generated. For each experimental condition, three biological replicates containing six independent composite plants were included. Libraries were sequenced on an Illumina NextSeq 500 platform with a 150-cycle sequencing kit and a configuration of pair-end reads with a 75 bp read length. Library construction and sequencing were performed by the Unidad Universitaria de Secuenciacin Masiva y Bioinformtica (Instituto de Biotecnologa, UNAM, Mxico). SRS12900077 Mature_Nodules_EV 21_Nodulos21d_EV3_GFL21_S39 RNA-Seq TRANSCRIPTOMIC cDNA NextSeq 500