SRX15168712 GSM6108316_r1 SRP373844 PRJNA835459 SRS12909767 GSM6108316 GSM6108316 RNA-Seq TRANSCRIPTOMIC cDNA For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168713 GSM6108317_r1 SRP373844 PRJNA835459 SRS12909768 GSM6108317 GSM6108317 RNA-Seq TRANSCRIPTOMIC cDNA For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168714 GSM6108318_r1 SRP373844 PRJNA835459 SRS12909769 GSM6108318 GSM6108318 RNA-Seq TRANSCRIPTOMIC cDNA For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168715 GSM6108319_r1 SRP373844 PRJNA835459 SRS12909770 GSM6108319 GSM6108319 RNA-Seq TRANSCRIPTOMIC cDNA For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168716 GSM6108320_r1 SRP373844 PRJNA835459 SRS12909771 GSM6108320 GSM6108320 RNA-Seq TRANSCRIPTOMIC cDNA For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168717 GSM6108321_r1 SRP373844 PRJNA835459 SRS12909772 GSM6108321 GSM6108321 RNA-Seq TRANSCRIPTOMIC cDNA For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168718 GSM6108322_r1 SRP373844 PRJNA835459 SRS12909773 GSM6108322 GSM6108322 RNA-Seq TRANSCRIPTOMIC cDNA For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168719 GSM6108323_r1 SRP373844 PRJNA835459 SRS12909774 GSM6108323 GSM6108323 RNA-Seq TRANSCRIPTOMIC cDNA For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168720 GSM6108324_r1 SRP373844 PRJNA835459 SRS12909775 GSM6108324 GSM6108324 RNA-Seq TRANSCRIPTOMIC cDNA For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168721 GSM6108325_r1 SRP373844 PRJNA835459 SRS12909776 GSM6108325 GSM6108325 RNA-Seq TRANSCRIPTOMIC cDNA For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168722 GSM6108326_r1 SRP373844 PRJNA835459 SRS12909777 GSM6108326 GSM6108326 RNA-Seq TRANSCRIPTOMIC cDNA For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168723 GSM6108327_r1 SRP373844 PRJNA835459 SRS12909778 GSM6108327 GSM6108327 RNA-Seq TRANSCRIPTOMIC cDNA For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168724 GSM6108328_r1 SRP373844 PRJNA835459 SRS12909779 GSM6108328 GSM6108328 OTHER GENOMIC other For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168725 GSM6108329_r1 SRP373844 PRJNA835459 SRS12909780 GSM6108329 GSM6108329 OTHER GENOMIC other For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168726 GSM6108330_r1 SRP373844 PRJNA835459 SRS12909781 GSM6108330 GSM6108330 OTHER GENOMIC other For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168727 GSM6108331_r1 SRP373844 PRJNA835459 SRS12909782 GSM6108331 GSM6108331 OTHER GENOMIC other For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168728 GSM6108332_r1 SRP373844 PRJNA835459 SRS12909783 GSM6108332 GSM6108332 OTHER GENOMIC other For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168729 GSM6108333_r1 SRP373844 PRJNA835459 SRS12909784 GSM6108333 GSM6108333 OTHER GENOMIC other For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168730 GSM6108334_r1 SRP373844 PRJNA835459 SRS12909785 GSM6108334 GSM6108334 OTHER GENOMIC other For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168731 GSM6108335_r1 SRP373844 PRJNA835459 SRS12909786 GSM6108335 GSM6108335 OTHER GENOMIC other For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168732 GSM6108336_r1 SRP373844 PRJNA835459 SRS12909787 GSM6108336 GSM6108336 OTHER GENOMIC other For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168733 GSM6108337_r1 SRP373844 PRJNA835459 SRS12909788 GSM6108337 GSM6108337 OTHER GENOMIC other For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168734 GSM6108338_r1 SRP373844 PRJNA835459 SRS12909789 GSM6108338 GSM6108338 OTHER GENOMIC other For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168735 GSM6108339_r1 SRP373844 PRJNA835459 SRS12909790 GSM6108339 GSM6108339 OTHER GENOMIC other For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168736 GSM6108340_r1 SRP373844 PRJNA835459 SRS12909791 GSM6108340 GSM6108340 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168737 GSM6108341_r1 SRP373844 PRJNA835459 SRS12909792 GSM6108341 GSM6108341 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168738 GSM6108342_r1 SRP373844 PRJNA835459 SRS12909793 GSM6108342 GSM6108342 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168739 GSM6108343_r1 SRP373844 PRJNA835459 SRS12909794 GSM6108343 GSM6108343 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168740 GSM6108344_r1 SRP373844 PRJNA835459 SRS12909795 GSM6108344 GSM6108344 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000 SRX15168741 GSM6108345_r1 SRP373844 PRJNA835459 SRS12909796 GSM6108345 GSM6108345 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA For RNAseq samples: LNCaP/AR cells transduced with different shRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. For scRNAseq samples: Based on FACS analysis, single cells were sorted into 1.5 ml tubes (Eppendorf) and cells were counted by Countess II FL automatic cell counter (Invitrogen) and cell viability was above 90%. The concentration of single cell suspensions was adjusted to 900-1100 cells/l. Cells were loaded between 10,000 and 17,000 cells/chip position using the Chromium Single cell 5' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics, V1 barcoding chemistry). For RNAseq samples: RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA. For scRNAseq samples: Single-cell gene expression libraries were generated according to the manufacturer's instructions. All the subsequent steps were performed following the standard manufacturer's protocols. For RNAseq samples: RNA-Seq. For scRNAseq samples: Single-cell RNA-seq were performed by the 10x Genomic single cell 5'library platform. Illumina NovaSeq 6000