SRX15246443 OL3ReECTzP SRP374998 bp0 Total metagenomic DNA was extracted from pools of thrips specimens of the same morphotypes (n = 10) obtained from the same avocado crop culturing farm using Total DNA was extracted using chemical lysis with 50mM Tris-HC1 buffer (pH 8.5), 1mM EDTA, 0.5% SDS , 200 g/ml and proteinase K followed by purification with FastDNA SPIN KIT (MP Biomedicals). DNA was quantified using Nanodrop 2000 (Thermo Fisher Scientific, USA). PCR amplification was performed using primers 515F (5'-GTGYCAGCMGCCGCGGTAA-3') [47] and 806R (5'-GGACTACNVGGGTWTCTAAT-3') [48] targeting the hypervariable region V4-V5 of 16S rRNA genes. The PCR products were used for Nextera TX library construction and were sequenced using Illumina MiSeq platform 2 250 bp. SRS12979342 IvzVi2OGpR_LVn_139 OL3ReECTzP AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246444 2ihTbpKC1p SRP374998 bp0 Total metagenomic DNA was extracted from pools of thrips specimens of the same morphotypes (n = 10) obtained from the same avocado crop culturing farm using Total DNA was extracted using chemical lysis with 50mM Tris-HC1 buffer (pH 8.5), 1mM EDTA, 0.5% SDS , 200 g/ml and proteinase K followed by purification with FastDNA SPIN KIT (MP Biomedicals). DNA was quantified using Nanodrop 2000 (Thermo Fisher Scientific, USA). PCR amplification was performed using primers 515F (5'-GTGYCAGCMGCCGCGGTAA-3') [47] and 806R (5'-GGACTACNVGGGTWTCTAAT-3') [48] targeting the hypervariable region V4-V5 of 16S rRNA genes. The PCR products were used for Nextera TX library construction and were sequenced using Illumina MiSeq platform 2 250 bp. SRS12979345 uum0ZyeE8q_LV2_151 2ihTbpKC1p AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246445 C8caid8NXR SRP374998 bp0 Total metagenomic DNA was extracted from pools of thrips specimens of the same morphotypes (n = 10) obtained from the same avocado crop culturing farm using Total DNA was extracted using chemical lysis with 50mM Tris-HC1 buffer (pH 8.5), 1mM EDTA, 0.5% SDS , 200 g/ml and proteinase K followed by purification with FastDNA SPIN KIT (MP Biomedicals). DNA was quantified using Nanodrop 2000 (Thermo Fisher Scientific, USA). PCR amplification was performed using primers 515F (5'-GTGYCAGCMGCCGCGGTAA-3') [47] and 806R (5'-GGACTACNVGGGTWTCTAAT-3') [48] targeting the hypervariable region V4-V5 of 16S rRNA genes. The PCR products were used for Nextera TX library construction and were sequenced using Illumina MiSeq platform 2 250 bp. SRS12979344 P3Fz2YZpm9_LS1_146 C8caid8NXR AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246446 MRpwLivWtH SRP374998 bp0 Total metagenomic DNA was extracted from pools of thrips specimens of the same morphotypes (n = 10) obtained from the same avocado crop culturing farm using Total DNA was extracted using chemical lysis with 50mM Tris-HC1 buffer (pH 8.5), 1mM EDTA, 0.5% SDS , 200 g/ml and proteinase K followed by purification with FastDNA SPIN KIT (MP Biomedicals). DNA was quantified using Nanodrop 2000 (Thermo Fisher Scientific, USA). PCR amplification was performed using primers 515F (5'-GTGYCAGCMGCCGCGGTAA-3') [47] and 806R (5'-GGACTACNVGGGTWTCTAAT-3') [48] targeting the hypervariable region V4-V5 of 16S rRNA genes. The PCR products were used for Nextera TX library construction and were sequenced using Illumina MiSeq platform 2 250 bp. SRS12979346 L4XldwdVN2_EP1_150 MRpwLivWtH AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246447 XPbBSrsKSy SRP374998 bp0 Total metagenomic DNA was extracted from pools of thrips specimens of the same morphotypes (n = 10) obtained from the same avocado crop culturing farm using Total DNA was extracted using chemical lysis with 50mM Tris-HC1 buffer (pH 8.5), 1mM EDTA, 0.5% SDS , 200 g/ml and proteinase K followed by purification with FastDNA SPIN KIT (MP Biomedicals). DNA was quantified using Nanodrop 2000 (Thermo Fisher Scientific, USA). PCR amplification was performed using primers 515F (5'-GTGYCAGCMGCCGCGGTAA-3') [47] and 806R (5'-GGACTACNVGGGTWTCTAAT-3') [48] targeting the hypervariable region V4-V5 of 16S rRNA genes. The PCR products were used for Nextera TX library construction and were sequenced using Illumina MiSeq platform 2 250 bp. SRS12979343 RCFZlISiLW_BH1_140 XPbBSrsKSy AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246448 gjEp0WR2jk SRP374998 bp0 Total metagenomic DNA was extracted from pools of thrips specimens of the same morphotypes (n = 10) obtained from the same avocado crop culturing farm using Total DNA was extracted using chemical lysis with 50mM Tris-HC1 buffer (pH 8.5), 1mM EDTA, 0.5% SDS , 200 g/ml and proteinase K followed by purification with FastDNA SPIN KIT (MP Biomedicals). DNA was quantified using Nanodrop 2000 (Thermo Fisher Scientific, USA). PCR amplification was performed using primers 515F (5'-GTGYCAGCMGCCGCGGTAA-3') [47] and 806R (5'-GGACTACNVGGGTWTCTAAT-3') [48] targeting the hypervariable region V4-V5 of 16S rRNA genes. The PCR products were used for Nextera TX library construction and were sequenced using Illumina MiSeq platform 2 250 bp. SRS12979348 ArbZ7FIsBz_AASS2_147 gjEp0WR2jk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246449 PQ9DDmPq20 SRP374998 bp0 Total metagenomic DNA was extracted from pools of thrips specimens of the same morphotypes (n = 10) obtained from the same avocado crop culturing farm using Total DNA was extracted using chemical lysis with 50mM Tris-HC1 buffer (pH 8.5), 1mM EDTA, 0.5% SDS , 200 g/ml and proteinase K followed by purification with FastDNA SPIN KIT (MP Biomedicals). DNA was quantified using Nanodrop 2000 (Thermo Fisher Scientific, USA). PCR amplification was performed using primers 515F (5'-GTGYCAGCMGCCGCGGTAA-3') [47] and 806R (5'-GGACTACNVGGGTWTCTAAT-3') [48] targeting the hypervariable region V4-V5 of 16S rRNA genes. The PCR products were used for Nextera TX library construction and were sequenced using Illumina MiSeq platform 2 250 bp. SRS12979347 i3uOUzdq6Z_AASS1_144 PQ9DDmPq20 AMPLICON METAGENOMIC PCR Illumina MiSeq