SRX15246505 clgm27 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979391 27 - LC1572 clgm27 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246506 clgm28 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979392 28 - LC25346 clgm28 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246507 clgm29 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979393 29 - LC1493 clgm29 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246508 clgm30 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979394 30 - LC1524 clgm30 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246509 clgm31 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979395 31 - LC1651 clgm31 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246510 clgm5 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979396 5 - LC25311 clgm5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246511 clgm32 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979397 32 - LC2343 clgm32 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246512 clgm33 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979398 33 - LC2522 clgm33 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246513 clgm34 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979399 34 - LC2760 clgm34 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246514 clgm35 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979400 35 - LC2956 clgm35 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246515 clgm36 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979401 36 - LC3006 clgm36 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246516 clgm37 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979402 37 - LC3069 clgm37 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246517 clgm38 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979403 38 - LC3087 clgm38 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246518 clgm39 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979404 39 - LC3244 clgm39 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246519 clgm6 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979405 6 - LC25312 clgm6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246520 clgm7 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979406 7 - LC25426 clgm7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246521 clgm2 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979407 2 - LC25272 clgm2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246522 clgm3 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979408 3 - LC25309 clgm3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246523 clgm12 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979409 12 - LC25536 clgm12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246524 clgm13 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979410 13 - LC2646 clgm13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246525 clgm14 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979411 14 - LC2651 clgm14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246526 clgm15 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979412 15 - LC25270 clgm15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246527 clgm16 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979413 16 - LC25308 clgm16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246528 clgm17 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979414 17 - LC1370 clgm17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246529 clgm18 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979415 18 - LC1371 clgm18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246530 clgm19 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979416 19 - LC1373 clgm19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246531 clgm20 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979417 20 - LC1376 clgm20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246532 clgm21 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979418 21 - LC1379 clgm21 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246533 clgm4 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979419 4 - LC25310 clgm4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246534 clgm22 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979420 22 - LC1380 clgm22 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246535 clgm23 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979421 23 - LC1381 clgm23 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246536 clgm24 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979422 24 - LC1382 clgm24 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246537 clgm25 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979423 25 - LC1397 clgm25 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246538 clgm26 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979424 26 - LC1494 clgm26 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246539 clgm8 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979425 8 - LC25427 clgm8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246540 clgm9 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979426 9 - LC25428 clgm9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246541 clgm10 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979427 10 - LC25429 clgm10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX15246542 clgm11 SRP374999 PRJNA837097 Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control. SRS12979428 11 - LC25436 clgm11 AMPLICON METAGENOMIC PCR Illumina MiSeq