SRP382380 PRJNA850621 GSE206417 Integrated Gut/Liver-on-a-Chip platform as in vitro human model of non-alcoholic fatty liver disease Non-alcoholic fatty liver disease (NAFLD) influence one of third population around the world. Until now, no effective treatments have been established due to the improper in vitro assays and experimental animal models. By co-culturing human gut and liver cell lines (CaCO2 and HepG2 cells, respectively) interconnected via the microfluidic closed medium circulation loop, we created a gut-liver-on-a-chip (iGLC) platform as an in vitro human model of the gut-liver axis (GLA) in initiation and progression of NAFLD. Overall design: We analyzed cDNA profiles amplified with PCR-cDNA Barcoding Kit generated from RNAs in CaCO2 human gut cells and HepG2 human hpatocellular carcinoma cells with mono- or co-cultured conditions treated with 1 mM of free fatty acids using Oxford Nanopore MiniON. GSE206417 pubmed 36959276